Biotechnological advances in Lilium

Bakhshaie, Mehdi; Khosravi, Solmaz; Azadi, Pejman; Bagheri, Hedayat; Tuyl, J.M. van

doi:10.1007/s00299-016-2017-8

Cited by 68 publications

(54 citation statements)

References 177 publications

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“…Improvement of lily accessions through conventional breeding is time-consuming, tedious, and cost-intensive due to the long time requirement (averaging three years) from seed-to-flower production [ 5 ]. Moreover, most lily cultivars are susceptible to many diseases, although a few commercial lily cultivars are resistant to Botrytis elliptica , Fusarium oxysporum , and LMoV (Lily mottle virus) [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Exploration and Exploitation of Novel SSR Markers for Candidate Transcription Factor Genes in Lilium Species

Biswas

Nath

Howlader

et al. 2018

Genes

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Lilies (Lilium sp.) are commercially important horticultural crops widely cultivated for their flowers and bulbs. Here, we conducted large-scale data mining of the lily transcriptome to develop transcription factor (TF)-associated microsatellite markers (TFSSRs). Among 216,768 unigenes extracted from our sequence data, 6966 unigenes harbored simple sequence repeats (SSRs). Seventy-one SSRs were associated with TF genes, and these were used to design primers and validate their potential as markers. These 71 SSRs were accomplished with 31 transcription factor families; including bHLH, MYB, C2H2, ERF, C3H, NAC, bZIP, and so on. Fourteen highly polymorphic SSRs were selected based on Polymorphic Information Content (PIC) values and used to study genetic diversity and population structure in lily accessions. Higher genetic diversity was observed in Longiflorum compared to Oriental and Asiatic populations. Lily accessions were divided into three sub-populations based in our structure analysis, and an un-rooted neighbor-joining tree effectively separated the accessions according to Asiatic, Oriental, and Longiflorum subgroups. Finally, we showed that 46 of the SSR-associated genes were differentially expressed in response to Botrytis elliptica infection. Thus, our newly developed TFSSR markers represent a powerful tool for large-scale genotyping, high-density and comparative mapping, marker-aided backcrossing, and molecular diversity analysis of Lilium sp.

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Section: Introductionmentioning

confidence: 99%

Exploration and Exploitation of Novel SSR Markers for Candidate Transcription Factor Genes in Lilium Species

Biswas

Nath

Howlader

et al. 2018

Genes

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“…However, when the callus is placed in suitable and controlled conditions, the differentiation of shoots, embryos, and roots can be achieved. Based on these culture systems, various studies on biochemical or cytological processes of growth attributed to cell enlargement, differentiation, and cell division have been conducted (Bakhshaie et al, 2016;Daneshvar, 2016a, 2016b;Jalali et al, 2012;Niazian et al, 2017). Moreover, various reactions of genotypes from one species to in vitro culture conditions have been observed regularly and has been explained to be due to variations in endogenous phytohormone levels or abilities to react differently to exogenously applied plant growth regulators (PGRs) (Bhojwani and Dantu, 2013;Hesami et al, 2017b).…”

Section: Callus Culturementioning

confidence: 99%

“…Many methods such as micropropagation, seed germination, and regeneration from callus, embryo rescue, and cryopreservation are introduced for conserving rare and endangered plants' germplasms. In vitro methods for the propagation and preservation of geophytes have many advantages such as fast multiplication and relatively low costs, and thus they attract much more attention than traditional methods such as vegetative propagation (Bakhshaie et al, 2016;Daneshvar, 2016a, 2016b;Hesami et al, 2017aHesami et al, , 2017bJalali et al, 2012;Mohammadi-Dehcheshmeh et al, 2007, 2008.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…var. gloriosoides Baker, a native and rare perennial bulbous plant that only exists at the 150-660 m altitudes in the northern part of Taiwan (Bakhshaie et al, 2016;Chang et al, 2000). Many propagation methods were employed to find a fast and applicable method of propagation for Fritillaria persica (Cakmak et al, 2016;Ebrahimie et al, 2006;Genders, 1973;Mohammadi-Dehcheshmeh et al, 2007;Rahimi et al, 2013) and such studies were conducted after a notice (warning) had been issued informing that this species should be protected (Uluğ et al, 2010).…”

Section: Somatic Embryogenesismentioning

confidence: 99%

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In vitro culture as a powerful method for conserving Iranian ornamental geophytes

Hesami¹,

Naderi²,

Yoosefzadeh-Najafabadi³

et al. 2018

bta

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Never before in history has the issue of preserving biodiversity been of such significance than today, to the extent that it has become an important global issue. Nowadays, humans have a massive impact on biodiversity whether in rural, urban, or wilderness settings. Besides, biodiversity today is also being significantly constrained by climate change and the introduction of new varieties of plants. Plant tissue culture is a method that can be useful for collecting, storing, and multiplicating of plant germplasms. Nowadays, many valuable germplasms are in danger of extinction, especially in Iran. Thus, there is a necessity to preserve valuable Iranian ornamental geophytes such as Oxalis articulata, Eminium jaegeri, Muscari kurdicum, Leopoldia tijtijensis, Gagea calcicola, Tulipa faribae, Gagea alexii, and Allium. In this study, plant tissue culture as well as in vitro conservation techniques as means for medium-and long-term conservation of Iranian ornamental geophytes is reviewed. There are only few studies on plant tissue culture of Iranian ornamental geophytes despite their great importance in Iran. To our knowledge, there is no report on cryopreservation of the abovementioned plants. In conclusion, the methods presented in this review can be utilized for conserving these valuable germplasms for future generations.

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“…In the United States, P. penetrans is considered one of the most damaging plant pathogens to Easter lily production (L. longiflorum cv. Nellie White), a floral crop with a pot plant wholesale value of approximately $26 million (Bakhsaie et al, 2016), whereas in South Korea lily production represents an annual value of $34 million (Kang et al, 2013). Disease symptoms on Easter lilies in the field are generally characterized by stunted shoot and root growth and chlorotic foliage.…”

mentioning

confidence: 99%

Characterization of Lilium longiflorum cv. ‘Nellie White’ Infection with Root-lesion Nematode Pratylenchus penetrans by Bright-field and Transmission Electron Microscopy

Vieira

Mowery

Kilcrease

et al. 2017

Journal of Nematology

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Lilium longiflorum cv. Nellie White, commonly known as Easter lily, is an important floral crop with an annual wholesale value of over $26 million in the United States. The root-lesion nematode, Pratylenchus penetrans, is a major pest of lily due to the significant root damage it causes. In this study, we investigated the cytological aspects of this plant-nematode interaction using bright-field and transmission electron microscopy. We took advantage of an in vitro culture method to multiply lilies and follow the nematode infection over time. Phenotypic reactions of roots inoculated with P. penetrans were evaluated from 0 to 60 d after nematode infection. Symptom development progressed from initial randomly distributed discrete necrotic areas to advanced necrosis along entire roots of each inoculated plant. A major feature characterizing this susceptible host response to nematode infection was the formation of necrosis, browning, and tissue death involving both root epidermis and cortical cells. Degradation of consecutive cell walls resulted in loss of cell pressure, lack of cytoplasmic integrity, followed by cell death along the intracellular path of the nematode's migration. Pratylenchus penetrans was never seen in the vascular cylinder as the layer of collapsed endodermal cells presumably blocked the progression of nematodes into this area of the roots. This study presents the first detailed cytological characterization of P. penetrans infection of Easter lily plants.

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Biotechnological advances in Lilium

Cited by 68 publications

References 177 publications

Exploration and Exploitation of Novel SSR Markers for Candidate Transcription Factor Genes in Lilium Species

Exploration and Exploitation of Novel SSR Markers for Candidate Transcription Factor Genes in Lilium Species

In vitro culture as a powerful method for conserving Iranian ornamental geophytes

Characterization of Lilium longiflorum cv. ‘Nellie White’ Infection with Root-lesion Nematode Pratylenchus penetrans by Bright-field and Transmission Electron Microscopy

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