Biotechnological potential of respiring Zymomonas mobilis: A stoichiometric analysis of its central metabolism

Pentjuss, Agris; Odzina, Ilona; Kostromins, Andrejs; Fell, David A.; Stalidzāns, Egils; Kalnenieks, Uldis

doi:10.1016/j.jbiotec.2013.02.014

Cited by 47 publications

(57 citation statements)

References 53 publications

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“…As a model bioethanol producer, Zymomonas mobilis has attracted considerable attention over the past decades due to its excellent industrial characteristics, such as the unique Entner-Doudoroff (ED) pathway under anaerobic conditions resulting in low cell mass formation, high specific rate of sugar uptake, high ethanol yield, notable ethanol tolerance, and the generally regarded as safe (GRAS) status (Panesar et al 2006;Rogers et al 2007). Furthermore, the availability of multiple genome sequences for 12 Zymomonas strains with small genome size around 2 Mb (Seo et al 2005;Yang et al 2009a;Zhao et al 2012), multiple genome-scale metabolic models (Kalnenieks et al 2014;Pentjuss et al 2013;Widiastuti et al 2011), and versatile genetic engineering strategies (Jia et al 2013;Shui et al 2015;Tan et al 2016) also accelerates the research progress in Z. mobilis. Z. mobilis has also been engineered for the production of sorbitol, gluconic acid, levan, 2,3-butanediol, isobutanol, and other biochemicals, which is proposed as an ideal microbial chassis for future synthetic biology and biorefinery (He et al 2014;Yang et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Yang

Tang

et al. 2018

Bioresour. Bioprocess.

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Pretreatment is the key step to overcome the recalcitrance of lignocellulosic biomass making sugars available for subsequent enzymatic hydrolysis and microbial fermentation. During the process of pretreatment and enzymatic hydrolysis as well as fermentation, various toxic compounds may be generated with strong inhibition on cell growth and the metabolic capacity of fermenting strains. Zymomonas mobilis is a natural ethanologenic bacterium with many desirable industrial characteristics, but it can also be severely affected by lignocellulosic hydrolysate inhibitors. In this review, analytical methods to identify and quantify potential inhibitory compounds generated during lignocellulose pretreatment and enzymatic hydrolysis were discussed. The effect of hydrolysate inhibitors on Z. mobilis was also summarized as well as corresponding approaches especially the high-throughput ones for the evaluation. Then the strategies to enhance inhibitor tolerance of Z. mobilis were presented, which include both forward and reverse genetics approaches such as classical and novel mutagenesis approaches, adaptive laboratory evolution, as well as genetic and metabolic engineering. Moreover, this review provided perspectives and guidelines for future developments of robust strains for efficient bioethanol or biochemical production from lignocellulosic materials.

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Section: Introductionmentioning

confidence: 99%

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Yang

Tang

et al. 2018

Bioresour. Bioprocess.

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“…This can occur because Zymomonas mobilis growth is independent of ethanol synthesis. Due to this peculiarity, Z. mobilis is considered to be an excellent ethanol producer (Yamashita et al, 2008;Pentjuss et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Zymomonas mobilis is highlighted in the literature as a promising microorganism for ethanol and other products of technological interest production such as the gum levan (or fructan) mainly due to its capacity to tolerate high ethanol and sugar concentrations, produce low biomass concentration, use the Entner-Doudoroff metabolic pathway for carbohydrate metabolism and show cell growth regardless of carbon consumption (Behera, Mohanty, & Ray, 2012;Pentjuss, Kostromins, Fell, Stalidzans, & Kalnenieks, 2013).…”

Section: Introductionmentioning

confidence: 99%

<b>Ethanol and Levan production by sequential bath using <i>Zymomonas mobilis</i> immobilized on alginate and chitosan beads

Santos

Cruz

2016

Acta Sci. Technol.

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ABSTRACT. Current study evaluates ethanol and levan production using Zymomonas mobilis immobilized on alginate and chitosan beads and assesses the capacity of the beads for reuse in sequential fermentations. Two experiments were carried out: In the first experiment 2 design evaluated the following independent variables: sucrose concentration; pH; incubation time; temperature and agitation. In the second experiment, based on the best conditions observed in 2 , the capacity of the immobilization support reuse in subsequent fermentations was evaluated for 12 days. In experiments using fractionated factorial experimental design 2 ). In batch sequential fermentation, re-using immobilization support, alginate support was efficient by maintaining the cellular viability on immobilized support (0.94 g L -1), levan production (21.11 g L -1) and ethanol production (87.21 g L -1). Chitosan support was inadequate for sequential batches since the beads dissolved after the second cycle.Keywords: biofuel, microbial polysaccharide, entrapment, fractionated factorial experiment.Produção de etanol e levana por batelada sequencial utilizando Zymomonas mobilis imobilizadas em esferas de alginato e quitosana RESUMO. O objetivo deste estudo foi avaliar a produção de etanol e levana por Zymomonas mobilis imobilizadas em esferas de alginato e quitosana e avaliar a capacidade de reutilização das esferas em fermentações sequenciais. Dois experimentos foram realizados: no primeiro, 2 foram avaliadas as variáveis independentes: concentração de sacarose; pH; tempo de incubação; temperatura e agitação. O segundo experimento foi realizado com base nas condições ótimas do ensaio 2 , onde foi avaliada a capacidade de reutilização dos suportes durante 12 dias. Para o experimento 2 , o suporte mais eficiente para a imobilização foi o alginato, com 2,6 g L ). A quitosana não foi adequada para a fermentação sequencial, uma vez que as esferas desintegraram-se após o segundo ciclo de reutilização do suporte.Palavras-chave: biocombustível, polissacarídeo microbiano, aprisionamento, planejamento fatorial fracionado.

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“…Often when optimizing flux distribution, only the best flux is assessed (Pentjuss, et al, 2013a). More advanced approach is to look for the best possible flux distribution, when biomass growth is taking place, to make the bioconversion more efficient.…”

Section: Introductionmentioning

confidence: 99%

“…The first stoichiometric models of Z. mobilis were published without available metabolic model files (Lee et al, 2010;Tsantili et al, 2007;Widiastuti et al, 2011) and therefore were not directly applicable for modeling and optimization. In this study the stoichiometric model of Z. mobilis central metabolism (Pentjuss, et al, 2013a) is used. The model does not include the fluxes from the central metabolism to biomass growth, and therefore would be valid for non-growing cells, e.g.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Zymomonas mobilis biotechnological potential in ethanol production by flux variability analysis

Pentjuss

Kalnenieks

2013

Bit-Journal

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Biotechnological potential of respiring Zymomonas mobilis: A stoichiometric analysis of its central metabolism

Cited by 47 publications

References 53 publications

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

<b>Ethanol and Levan production by sequential bath using <i>Zymomonas mobilis</i> immobilized on alginate and chitosan beads

Assessment of Zymomonas mobilis biotechnological potential in ethanol production by flux variability analysis

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