Biotechnological processes to obtain bioactive secondary metabolites from some Mexican medicinal plants

Pérez-González, Mariana Z.; Jiménez-Arellanes, María Adelina

doi:10.1007/s00253-021-11471-z

Cited by 4 publications

(2 citation statements)

References 134 publications

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“…In this regard, through the application of plant cell culture technology, it has been possible to obtain and improve the biomass and bioactive production of compounds, and there are several reports in which important results have been obtained, highlighting some advantages such as constant and controlled production in reduced spaces [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ]. Moreover, using biotic and abiotic elicitors, it is possible to increase compound production; in particular, light conditions have been studied as an elicitor to increase biomass production, and bioactive compounds such as alkaloids and phenolics [ 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Obtaining 2,3-Dihydrobenzofuran and 3-Epilupeol from Ageratina pichinchensis (Kunth) R.King & Ho.Rob. Cell Cultures Grown in Shake Flasks under Photoperiod and Darkness, and Its Scale-Up to an Airlift Bioreactor for Enhanced Production

et al. 2023

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Ageratina pichinchensis (Kunth) R.King & Ho.Rob. is a plant used in traditional Mexican medicine, and some biotechnological studies have shown that its calluses and cell suspension cultures can produce important anti-inflammatory compounds. In this study, we established a cell culture of A. pichinchensis in a 2 L airlift bioreactor and evaluated the production of the anti-inflammatory compounds 2,3-dihydrobenzofuran (1) and 3-epilupeol (2). The maximum biomass production (11.90 ± 2.48 g/L) was reached at 11 days of culture and cell viability was between 80% and 90%. Among kinetic parameters, the specific growth rate (µ) was 0.2216 days−1 and doubling time (td) was 3.13 days. Gas chromatography coupled with mass spectrometry (GC-MS) analysis of extracts showed the maximum production of compound 1 (903.02 ± 41.06 µg/g extract) and compound 2 (561.63 ± 10.63 µg/g extract) at 7 and 14 days, respectively. This study stands out for the significant production of 2,3-dihydrobenzofuran and 3-epilupeol and by the significant reduction in production time compared to callus and cell suspension cultures, previously reported. To date, these compounds have not been found in the wild plant, i.e., its production has only been reported in cell cultures of A. pichinchensis. Therefore, plant cell cultured in an airlift reactor can be an alternative for the improved production of these anti-inflammatory compounds.

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Section: Introductionmentioning

confidence: 99%

Obtaining 2,3-Dihydrobenzofuran and 3-Epilupeol from Ageratina pichinchensis (Kunth) R.King & Ho.Rob. Cell Cultures Grown in Shake Flasks under Photoperiod and Darkness, and Its Scale-Up to an Airlift Bioreactor for Enhanced Production

et al. 2023

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“…According to Romanova and Sweedler (2018), although the most significant percentage of animal research in neuroscience is conducted using mammalian model systems, the importance of other vertebrate and invertebrate models is well recognized. Drosophila sp, Caenorhabditis elegans, Dario rerio, yeast models, and other organisms have been used to gain insights into how proteins implicated in these neurodegenerative disorders cause cellular and organism pathology toxicity (Dawson et al, 2018;da Silva et al, 2021;Phulara et al, 2021;Pradhan et al, 2021;Cao et al, 2018;Pérez-González & Jiménez-Arellanes, 2021;Tello et al, 2022). Herein, we aimed to describe the protective effects of H. sucuuba against oxidative stress-mediated by Paraquat exposure using Drosophila melanogaster as a model.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of Amazonian Himatanthus sucuuba extracts in Drosophila melanogaster exposed to Paraquat

Silva

Lisboa

Santos

et al. 2022

RSD

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The Amazon rainforest is an essential source of scientific knowledge, so several research groups seek to understand the role of Amazonian compounds in diseases. Among the vast flora, Himatanthus sucuuba has a variety of therapeutic purposes and is used for the first time in the neuroprotection induced by Paraquat (PQ) in Drosophila melanogaster. In our study, we carried out phytochemical assays with the hydroalcoholic extract of H. sucuuba, revealing qualitatively classes of secondary metabolites and quantitatively total phenols (43.33 mg GAE/g extract-1), total flavonoids (44.09 mg GAE/G Extract- 1) and antioxidant activity via DPPH and ABTS. Furthermore, exposure of adult D. melanogaster (wild strain, Canton Special) to PQ for 15 days caused increased oxidative stress, as evidenced by elevated levels of protein carbonyls, lactate, and acetylcholinesterase and citrate synthase activities. However, the diet supplemented with H. sucuuba (0.1 mg/mL) for 15 days prevented damage from oxidative stress triggered by PQ. Our study aims to demonstrate the protective effect of H. sucuuba extract on D. melanogaster exposed to PQ. Based on our results, we suggest that extracts from the bark of H. sucuuba can prevent or minimize human diseases caused by oxidative stress. Therefore, further studies on the mechanisms involved in such activities will be necessary.

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Photoprotective effect of topical treatment with Lopezia racemosa extract against deleterious UVB irradiation effects in the skin of hairless mice

Erick,

Montserrat,

Antonio

et al. 2024

Photochem & Photobiology

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Lopezia racemosa is known as a “mosquito flower or perlilla.” It is commonly found in corn crops. In traditional Mexican medicine, this plant is used to treat stomach cancer and urinary tract infections. Likewise, compounds and extracts isolated from plants have shown cytotoxic and anti‐inflammatory effects. The objective of this study was to evaluate the photochemoprotective effect of topical treatment with the methanolic extract of L. racemosa (MELR) as a photochemoprotective agent against the harmful effects of UV irradiation (UVR) on a bacterial model and hairless mice. The MELR components were separated and analyzed via HPLC‐UV‐ESI‐MS. Antioxidant activity was evaluated by the ability of MERL to scavenge DPPH and ABTS free radicals and by its FRAP capacity. The toxicity of MELR was evaluated in keratinocyte cultures. The photoprotective capacity of MELR was assessed through challenge experiments using models with bacteria and hairless CD1 et/et mice; cytokines related to the damage caused by UVR were also measured. In the methanolic extract of L. racemosa, five metabolites were detected and identified: two isomers of quercetin 6‐C glycoside, orientin, quercetin 3‐(6″‐acetylglycoside) and quercetin 3‐(6″‐galloylglycoside) 7‐(2,3‐dihydroxytetrahydro‐2H‐pyran‐4‐yl acetate). MELR exhibited DPPH and ABTS radical scavenging properties, in addition to Fe ion reducing activity. MELR showed a photoprotective effect against UVB radiation‐induced death in Escherichia coli bacteria. At the histological level, topical treatment of CD‐1 et/et mice with MERL reduced the damage caused by UVR. Quantification of interleukins in the blood of mice revealed that the expression of IL‐12 was greater in the control group treated with ultraviolet radiation than in the group protected with MELR. The methanolic extract of L. racemosa has photochemoprotective properties.

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Biotechnological processes to obtain bioactive secondary metabolites from some Mexican medicinal plants

Cited by 4 publications

References 134 publications

Obtaining 2,3-Dihydrobenzofuran and 3-Epilupeol from Ageratina pichinchensis (Kunth) R.King & Ho.Rob. Cell Cultures Grown in Shake Flasks under Photoperiod and Darkness, and Its Scale-Up to an Airlift Bioreactor for Enhanced Production

Obtaining 2,3-Dihydrobenzofuran and 3-Epilupeol from Ageratina pichinchensis (Kunth) R.King & Ho.Rob. Cell Cultures Grown in Shake Flasks under Photoperiod and Darkness, and Its Scale-Up to an Airlift Bioreactor for Enhanced Production

Protective effect of Amazonian Himatanthus sucuuba extracts in Drosophila melanogaster exposed to Paraquat

Photoprotective effect of topical treatment with Lopezia racemosa extract against deleterious UVB irradiation effects in the skin of hairless mice

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