Biotechnology-Based Methods for the Detection, Enumeration and Epidemiology of Food Poisoning and Spoilage Organisms

Dodd, C.E.R.; Stewart, Gordon S. A. B.; Waites, W. M.

doi:10.1080/02648725.1990.10647864

Cited by 16 publications

(2 citation statements)

References 198 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several detection and enumeration methods based on nucleic acid hybridization have been reported for monitoring specific microorganisms in environmental samples (Bsat et al, 1994;Dodd at al., 1990;Sayler and Layton, 1990). Among them, the polymerase chain reaction (PCR), which can amplify unique sequences of DNA, provides very accurate, explicit, and rapid detection of microbial species in food or dairy products (Bsat et al, 1994;Saiki et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Improving Detection of Vibrio vulnificus in Octopus variabilis by PCR

Lee

Eun

Choi

1997

Journal of Food Science

View full text Add to dashboard Cite

PCR methods can detect foodborne pathogenic bacteria with simplicity, specificity and speed. In order to improve sensitivity and speed of PCR methods for detection of Vibrio vulnificus in small octopus homogenate, several media and culture conditions were compared. Modified brain heart infusion media containing 2% NaCl and adjusted to pH 8.0 and 30ЊC was most effective for enrichment of the bacteria. Procedures affecting the efficiency of template DNA extraction and target DNA amplification were also optimized. By these combined efforts, a PCR procedure capable of detecting V. vulnificus as low as 10 cells/mL within 10h was developed.

show abstract

Section: Introductionmentioning

confidence: 99%

Improving Detection of Vibrio vulnificus in Octopus variabilis by PCR

Lee

Eun

Choi

1997

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…Several reviews on the applications of luminescence have been published (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19), and many of these applications have relevance to food safety microbiologists.…”

mentioning

confidence: 99%

How Novel Methods Can Help Discover More Information about Foodborne Pathogens

Griffiths

2000

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

Considerable emphasis is being placed on quantitative risk assessment modelling as a basis for regulation of trade in food products. However, for models to be accurate, information about the behaviour of potential pathogens in foods needs to be available. The question is how to obtain this knowledge in a simple and cost effective way. One technique that has great potential is the use of reporter bacteria which have been genetically modified to express a phenotype that can be easily monitored, such as light production in luminescent organisms. Bacteria carrying these (lux) genes can easily be detected using simple luminometers or more sophisticated low light imaging equipment.By monitoring light output from these bacteria over time, it can easily be determined if the organism is growing (resulting in an increase in light emission), is dead (causing a decrease in light production) or is injured (light output remains constant). The use of imaging systems allows the response of bioluminescent bacteria to be studied directly on the food, making the technique even more useful. Applications of bioluminescence are discussed below and include use as reporters of gene expression; biocide efficacy and antibiotic susceptibility; sub-lethal injury; adhesion and biofilm formation; the microbial ecology of foods; pathogenesis; and as biosensors.

show abstract

In Memoriam: Gordon Sydney Anderson Birnie Stewart (1951-1999)

Hill

Stanley²

1999

Luminescence

View full text Add to dashboard Cite

Biotechnology-Based Methods for the Detection, Enumeration and Epidemiology of Food Poisoning and Spoilage Organisms

Cited by 16 publications

References 198 publications

Improving Detection of Vibrio vulnificus in Octopus variabilis by PCR

Improving Detection of Vibrio vulnificus in Octopus variabilis by PCR

How Novel Methods Can Help Discover More Information about Foodborne Pathogens

In Memoriam: Gordon Sydney Anderson Birnie Stewart (1951-1999)

Contact Info

Product

Resources

About