Bioterrorism preparedness: a state public health and molecular approach

Abstract

CHAPTERl.GENERALINTRODUCTION 1 CHAPTER 2. HISTORY OF BIOLOGICAL WARFARE Background Biological Warfare during the Middle Ages Exploration of the "New World" World Wars I and II 7 Post World War II BW activities by the United States 11 Alleged BT activities during the Korean War 14 Microbial and BW Stimulants during the 1950's and 1960's 19 Bacterial Organisms Proposed In BW Use by The CDC's Strategic Working Group Anthrax Plague Tularemia Clostridium botulinum toxin Smallpox Viral Hemorrhagic Fever Ebola and Marburg Viruses Overview of Salmonella CHAPTER 2. OVERVIEW OF MOLECULAR SUBTYPING CHAPTER 3. PFGE EXPERIMENTS Materials and Methods Preparation of Chromosomal DNA Restriction endonuclease digestion and PFGE Results Discussion 110 Conclusions 111 iv CHAPTER 4. A Three-Year Study (2001-2003) of Bioterrorism Preparedness in State Health Departments: Accomplishments and Dilemmas Sullllilary Article (In MMWR format) Discussion Conclusions APPENDIX 1. Resistance if Salmonella isolates tested APPENDIX 2. Grouping of antibiotic resistance profiles APPENDIX 3. Serotyping, PFGE profiles and antibiotic susceptibility testing