Biotests in Ecotoxicology: Current Practice and Problems

Lyubenova, Mariyana; Boteva, Silvena

doi:10.5772/64776

Cited by 12 publications

(9 citation statements)

References 90 publications

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“…The most used biological systems were crustaceans and fish followed by insects, mollusks, algae, bacteria and plants. The mono-species tests were the most used in research studies during 2010 -2016 followed by multispecies test and twospecies test and in finally community-cosm [1]. This was happening because single species tests were in the routine of laboratories, easy to be reproduced and more economic.…”

Section: Trend Of Ecotoxicity Studiesmentioning

confidence: 99%

“…The interest of scientists to identify, solve and prevent the environmental problems has become a priority in the context of population growth, waters contamination, global warming and ecosystems damage or their extinction [1]. Moreover, the human well-being led to chemical market expansion that have generated new anthropogenic contaminants in larger amounts, thus enhancing their negative impact on the environment.…”

mentioning

confidence: 99%

“…Ecotoxicology field is a complex scientific discipline base on principles of toxicology, biology, chemistry and ecology, being focused on the research of chemical compounds effects on living organisms, other than man. The ecotoxicology is separated in two section: pollutant dynamicsthat evaluate the mobility, migration, transformation, degradation in different environmental area; and bio toxicology -that examine the effects of toxic compounds on sensitive biological systems correlated with field data [1]. This review intends to highlight the progress of Romanian research, especially from National Research and Development Institute for Industrial Ecology -ECOIND Bucharest, in the last 10 years in the field of anthropogenic pollutants ecotoxicology and their aquatic risk assessment.…”

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confidence: 99%

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Anthropogenic Pollutants: 10 Years of Progress in Ecotoxicological Studies and Aquatic Risk Assessment

Gheorghe

Lucaciu

Păun

et al. 2019

RJEEC

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The market expansion due to globalization generated more anthropogenic contaminants, enhancing their negative impact on the environment. In order to prevent and balanced their negative effect, more rapid, sensitive and eco-friendly ecotoxicological studies as risk assessments were developed. Each country developed national programs to ensure the research infrastructure for implementation and development of new testing methodologies, generating new experimental data transferable to governmental authorities, economic agents and academia. In this review, we intend to highlighted the progress of Romanian researchers in the last 10 years in the field of ecotoxicology and aquatic risk assessment. The studies focused on aquatic systems as a non-targeted system to the anthropogenic pollutants impact. The implemented methodologies according with international requirements for chemical control like REACH Regulation, Detergents Regulation, Biocide Products Regulation or the Aquatic Risk Assessment Guidelines were presented. The ecotoxicological effects of various contaminants such as detergents, pharmaceuticals, pesticides, biocides or heavy metals and environmental polluted samples (surface water, sediment or sludge) were provided. Overall, the studies presented in our studies have a nationally novelty through the variety of test substances, the complexity of laboratory experiments and data processing. The research work has a continue applicability in industry and for control authorities. New accredited testing services that providing useful information about the chemicals and their impact on the environmental were introduced. In addition, the ecotoxicology studies and their challenges were a great source for professional training like PhD and master studies and for impact published papers.

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Section: Trend Of Ecotoxicity Studiesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Anthropogenic Pollutants: 10 Years of Progress in Ecotoxicological Studies and Aquatic Risk Assessment

Gheorghe

Lucaciu

Păun

et al. 2019

RJEEC

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“…Acute ecotoxicological test examinations are the first step to detect the total toxic effects caused by toxicant, and virtually every hydrobiont is suitable for conducting the tests [38]. The assessment of the environmental risk values helped to determine whether the toxicant is biologically active at test doses and to define LC 50 , which proved to be lethal causing death to 50% of the tested organisms [44].…”

Section: Toxicity Identification Evaluations (Ties) With Pseudoraspormentioning

confidence: 99%

“…The waste water mixers frequently contain detergents with high portion of phosphorus causing eutrophication [12,50]. It has been reported that the presence of 1 mg/l detergents in water causes plankton perishes; 3 mg/l -mass Daphne death; 5 mg/l -fish damage [38]. In concentrations between 0.4 and 40 mg/l synthetic detergents are reported to be acutely toxic to fish [1].…”

Section: Introductionmentioning

confidence: 99%

Ecotoxicity of Purified Industrial Waste Water

Lyubenova¹,

Dineva²,

Cala³

et al. 2019

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Purified industrial waste water (PIWW) has been evaluated for probable toxicity using test-systems with Pseudorasbora parva (topmouth gudgeon) and Lepidium sativum L (garden cress). The acute toxicity of PIWW was calculated according to mortality of Pseudorasbora parva in dilutions 1, 5, 10, 25, 50, 100 and 200 times, and distillate water (DW) was used as a control. The LC 50 has been calculated after 96 h of treatment and it was at approximately 8x (7.69) times dilution of PIWW. The toxic effect of PIWW with and without copper ions, added as CuSO 4 have been measured using Lepidium sativum L. The comparison of the toxic effects of the same concentrations of copper in PIWW and DW, mixed and non-mixed contamination has been evaluated. It was found that Cu +2 has inhibitory effects on the root's and stem's growth of Lepidium sativum L seeds, and that effect appears in concentrations over 2 mg/l Cu +2 . The 50% inhibition of root's growth in DW was EC 50 =7.26 mg/l of copper ions, while for PIWW that concentration was EC 50 =17.23 mg/l Cu +2 . The calculated EC 50 for stem's growing in DW was 54.57 mg/l Cu +2 and 72.07 mg/l Cu +2 in PIWW. The observed EC 50 differences in DW and PIWW perhaps are due to the formation of ligand compounds among copper cations and other impurities in the waste water and hence as consequences the reducing of free Cu +2 or their bioavailability, hereafter reduce copper toxicity. It was registered that PIWW diminished growth inhibitory effect of copper ions on Lepidium sativum L seeds lessening its amount by involving free Cu +2 in complexes with other waste products.

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