Biotin‐modified glutathione as a functionalized coating for bioconjugation of CdTe‐based quantum dots

Abstract

In this study, biotin-conjugated glutathione was synthesized using peptide bonding of the biotin carboxy group and amino group of the γ-glutamic acid to prepare an alternative coating for CdTe quantum dots (QDs). This type of coating combines the functionality of the biotin with the fluorescent properties of the QDs to create a specific, high-affinity fluorescent probe able to react with avidin, streptavidin and/or neutravidin. Biotin-functionalized glutathione-coated CdTe QDs were prepared by a simple one-step method using Na₂ TeO₃ and CdCl₂. Obtained QDs were separated from the excess of the biotin-conjugated glutathione by CE employing 300 mM borate buffer with pH 7.8 as a background electrolyte. The detection of sample components was performed by the photometric detection at 214 nm and LIF employing Ar⁺ ion laser (488 nm).

SDS-PAGE as a Tool for Hydrodynamic Diameter-Dependent Separation of Quantum Dots

“…They have unique spectral properties featured mainly by the hydrodynamic diameter and/or composition-tuneable emissions due to quantum size effects [1][2][3][4]. The range of their use varies from chemical to physical, computing and biological sciences [5].…”

Analysis and applications of nanoparticles in capillary electrophoresis

“…The characterization of NPs that are conjugated with compounds such as antibodies or oligonucleotides has also been effectively conducted using CE [50]. Ryvolova et al conducted an analysis of synthetic biotin-functionalized, glutathione-coated QDs (B-GSH-QDs) by CE with UV spectroscopy and LIF [51]. They separated glutathione (GSH), biotinylated glutathione (B-GSH), and B-GSH-QD, and they monitored the stability of B-GSH-QD during storage.…”

Quantum dots and prion proteins

“…Modification of QDs by the streptavidin proved a very successful method evaluated in numerous publications. 78,[95][96][97][98] Due to this success, streptavidin-QDs are nowadays also commercially available. In addition, biotin-functionalized QDs were developed to exploit the same interaction (Fig.…”