Biotypes, Serovars and Antimicrobial Resistance Patterns of Acinetobacter baumannii Clinical Isolates

Oliveira, Murilo Gomes; Irino, Kinue; Vaz, Tânia; Gonçalves, Célia R.; Levy, Carlos Emílio

doi:10.1016/s0934-8840(96)80006-5

Cited by 2 publications

(4 citation statements)

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“…As previously reported 8 , biotyping proved to be an easy and appropriated method for screening strains. This method differentiated our 76 strains into six categories with predominance of biotypes 2, 6 and 9 which were already reported as frequently recovered from clinical specimens 6,8,19,29 . Heterogeneity of strains of same biotype was further identified with serotyping.…”

Section: Discussionmentioning

confidence: 99%

“…The same observation was made on the biotype 6 strains which were associated with six different serotypes with the predominance of the biotype 6/ serotype O13 association. OLIVEIRA et al 19 , showed that strains of A. baumannii belonging to biotype 2/serotype O29 as well as strains of biotype 6/serotype O13 were predominant among strains isolated both from inpatients and outpatients of two hospitals in São Paulo, Brazil. It is worth noting that serotype O29 was recently identified among Brazilian strains as one of the prevalent serotypes 19,29 .…”

Section: Discussionmentioning

confidence: 99%

“…OLIVEIRA et al 19 , showed that strains of A. baumannii belonging to biotype 2/serotype O29 as well as strains of biotype 6/serotype O13 were predominant among strains isolated both from inpatients and outpatients of two hospitals in São Paulo, Brazil. It is worth noting that serotype O29 was recently identified among Brazilian strains as one of the prevalent serotypes 19,29 . Although rarely applied 19,28,29 serotyping in combination with other methods can be useful for epidemiological studies.…”

Section: Discussionmentioning

confidence: 99%

“…It is worth noting that serotype O29 was recently identified among Brazilian strains as one of the prevalent serotypes 19,29 . Although rarely applied 19,28,29 serotyping in combination with other methods can be useful for epidemiological studies.…”

Section: Discussionmentioning

confidence: 99%

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Biotyping, serotyping and ribotyping as epidemiological tools in the evaluation of Acinetobacter baumannii dissemination in hospital units, Sorocaba, São Paulo, Brazil

Gonçalves

Vaz

Araújo

et al. 2000

Rev. Inst. Med. trop. S. Paulo

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Dissemination of Acinetobacter baumannii strains in different units of a hospital in Sorocaba, São Paulo, Brazil was evaluated over a period of two years. By using biotyping, serotyping and ribotyping, 27 distinct clones were differentiated among 76 strains isolated between 1993-94, from clinical specimens of hospitalized patients. Two clones, 2:O4:A (biotype:serotype:ribotype) and 2:O29:A accounted for the majority of strains widely disseminated in the units during 1993. The introduction in the hospital setting, of a new clone, 6:O13:B, at the end of 1993 and its predominance through 1994 is discussed. Among 15 strains isolated from neonates, 6 (40%) belonged to the same clone, 2:O4:A. Interestingly, this clone was almost all recovered in neonatal intensive care unit, nursery and in pediatric unit. All strains were susceptible to imipenem and polymyxcin B. Multiresistant strains (up to 12 antimicrobial agents) accounted for 66.7% and 84.8% of the strains isolated in 1993 and in 1994, respectively.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Biotyping, serotyping and ribotyping as epidemiological tools in the evaluation of Acinetobacter baumannii dissemination in hospital units, Sorocaba, São Paulo, Brazil

Gonçalves

Vaz

Araújo

et al. 2000

Rev. Inst. Med. trop. S. Paulo

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Photoinactivation of Acinetobacter baumannii and Escherichia coli B by a Cationic Hydrophilic Porphyrin at Various Light Wavelengths

2001

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Photodynamic treatment by the cationic TMPyP photosensitizer was undertaken on the multiple antibiotic-resistant bacteria Acinetobacter baumannii and Escherichia coli. Total eradication of the bacterial cultures was determined immediately after initiation of illumination when these bacteria were treated with 5, 10, 15, 20-tetra (4-N methylpyridyl)porphine (TMPyP) at a concentration of 29.4 micromol/L and illuminated by blue, green, or red light. Total eradication of both bacteria was obtained also after treatment of bacterial cultures with 3.7 micromol/L TMPyP and illumination with blue light (400-450 nm). On the other hand, an 8- or 16- to 20-fold higher light intensity, respectively, was required for total eradication upon illumination with green (480-550 nm) or red light (600-700 nm). A 407-nm blue light only 7 and 9 joules/cm2, respectively, was needed for total eradication of both bacteria even at a concentration of 3.7 micromol/L TMPyP. X-ray-linked microanalysis demonstrated loss of potassium and a flood of sodium and chloride into the cells, indicating serious damage to the cytoplasmic membrane. Transmission electron microscopy (TEM) revealed structural changes and damage to the membrane of treated E. coli. In A. baumannii-treated cells, mesosomes and black dots that resemble aggregation of polyphosphate polymers could be seen. DNA breakage appeared only after a long period of illumination, when the bacterial cell was no longer viable. It can be concluded that cytoplasmic membrane damage and not DNA breakage is the major cause for bacterial death upon photosensitization.

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Biotypes, Serovars and Antimicrobial Resistance Patterns of Acinetobacter baumannii Clinical Isolates

Cited by 2 publications

References 20 publications

Biotyping, serotyping and ribotyping as epidemiological tools in the evaluation of Acinetobacter baumannii dissemination in hospital units, Sorocaba, São Paulo, Brazil

Biotyping, serotyping and ribotyping as epidemiological tools in the evaluation of Acinetobacter baumannii dissemination in hospital units, Sorocaba, São Paulo, Brazil

Photoinactivation of Acinetobacter baumannii and Escherichia coli B by a Cationic Hydrophilic Porphyrin at Various Light Wavelengths

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