Biphasic changes in TGF-β1 signaling drive NSAID-induced multi-organ damage

Chakraborti, Sreemoyee; Pramanick, Arnab; Saha, Sudipta; Sarkar, Subhasish; Singh, Laishram Pradeepkumar; Stewart, Adele; Maity, Biswanath

doi:10.1016/j.freeradbiomed.2020.06.026

Cited by 9 publications

(12 citation statements)

References 62 publications

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“…After 8 h, the cells were washed with chilled PBS solution twice, further fixed in 4 % paraformaldehyde at RT for 10 min and mounted with Vectashield mounting media (Invitrogen). Then cells were visualized by fluorescence microscopy (Optika B‐100FL HBO, Italy) for labelled fluorescence [69] …”

Section: Methodsmentioning

confidence: 99%

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Generation of Self‐Assembled Structures Composed of Amphipathic, Charged Tripeptides for Intracellular Delivery of Pro‐Apoptotic Chemotherapeutics

Sivagnanam

Das

Sivakadatcham

et al. 2022

Israel Journal of Chemistry

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Chemotherapeutic drugs remain the most efficacious treatment options for a many human cancers. However, the inability to deliver these drugs directly to cancerous cells often results in dose limiting and sometimes life-threatening adverse effects. Rather than developing new chemical moieties, researchers have begun focusing on the development of drug carriers, which are specifically designed to shuttle chemotherapeutics into malignant cells while sparing healthy cells. Charged nanoparticles have emerged as effective delivery platforms for several xenobiotic classes including anticancer agents, oligonucleotides and antibodies. Notably, peptide-based self-assembled nanostructures are of particular interest due to their biocompatibility, high drug loading capacity, and potential for customization for cellspecific targeting. We synthesized and studied the selfassembling properties of two charged, cell penetrating tri-peptides: H 2 NÀ ArgÀ LeuÀ PheÀ OMe (S1) with Arg as cationic amino acid and BocÀ PheÀ LeuÀ GluÀ OH (S2) with Glu as negatively charged amino acid. The fibrils and spherical selfassembled structures formed by S1 and S2, respectively, can encapsulate the chemotherapeutic drug Doxorubicin and facilitate intracellular drug delivery via endocytosis. Furthermore, S1-and S2-encapsulated Doxorubicin (Dox-S1, Dox-S2), like the unencapsulated drug, induced oxidative stress and mitochondrial dysfunction, activated the ATM/p53 signaling cascades, and triggered apoptosis in cancer cells. Thus, while the surface charge of molecular building blocks used to generate supramolecular assemblies influences the morphology of generated nanostructures, both cationic and anionic peptide-based assemblies are capable of functioning as drug delivery vehicles.

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Section: Methodsmentioning

confidence: 99%

“…Then cells were visualized by fluorescence microscopy (Optika B-100FL HBO, Italy) for labelled fluorescence. [69]…”

Section: Dna Fragmentation Assaymentioning

confidence: 99%

Generation of Self‐Assembled Structures Composed of Amphipathic, Charged Tripeptides for Intracellular Delivery of Pro‐Apoptotic Chemotherapeutics

Sivagnanam

Das

Sivakadatcham

et al. 2022

Israel Journal of Chemistry

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“…Then cells were visualized by uorescence microscopy (Optika B-100FL HBO, Italy) for labelled uorescence. 63 Comet assay…”

Section: Dna Fragmentation Assaymentioning

confidence: 99%

“…PS2-Zn-Aptamer was also used for microscopic studies in a separate set of experiments. Then cells were visualized by fluorescence microscopy (Optika B-100FL HBO, Italy) for labelled fluorescence 63.…”

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confidence: 99%

Self-assembled dipeptide based fluorescent nanoparticles as a platform for developing cellular imaging probes and targeted drug delivery chaperones

et al. 2022

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“…The next day, they were treated for 3 h at room temperature with anti-rabbit secondary antibodies conjugated to horseradish peroxidase at 1:3,000 dilutions. After washing the film thrice with TBS-T, the PVDF membrane was developed using enhanced chemiluminescence ECL (PierceTM ECL Western Blotting Substrate), and the images were recorded using Chemidoc (Chakraborti et al, 2018;Basak et al, 2020;Chakraborti et al, 2020).…”

Section: Western Blot Analysismentioning

confidence: 99%

Preclinical Evaluation of Dimethyl Itaconate Against Hepatocellular Carcinoma via Activation of the e/iNOS-Mediated NF-κB–Dependent Apoptotic Pathway

et al. 2022

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Hepatocellular carcinoma (HCC) is one of the most common tumors affecting a large population worldwide, with the fifth and seventh greatest mortality rates among men and women, respectively, and the third prime cause of mortality among cancer victims. Dimethyl itaconate (DI) has been reported to be efficacious in colorectal cancer by decreasing IL-1β release from intestinal epithelial cells. In this study, diethylnitrosamine (DEN)-induced HCC in male albino Wistar rats was treated with DI as an anticancer drug. The function and molecular mechanism of DI against HCC in vivo were assessed using histopathology, enzyme-linked immunosorbent assay (ELISA), and Western blot studies. Metabolomics using 1H-NMR was used to investigate metabolic profiles. As per molecular insights, DI has the ability to trigger mitochondrial apoptosis through iNOS- and eNOS-induced activation of the NF-κB/Bcl-2 family of proteins, CytC, caspase-3, and caspase-9 signaling cascade. Serum metabolomics investigations using 1H-NMR revealed that aberrant metabolites in DEN-induced HCC rats were restored to normal following DI therapy. Furthermore, our data revealed that the DI worked as an anti-HCC agent. The anticancer activity of DI was shown to be equivalent to that of the commercial chemotherapeutic drug 5-fluorouracil.

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Biphasic changes in TGF-β1 signaling drive NSAID-induced multi-organ damage

Cited by 9 publications

References 62 publications

Generation of Self‐Assembled Structures Composed of Amphipathic, Charged Tripeptides for Intracellular Delivery of Pro‐Apoptotic Chemotherapeutics

Generation of Self‐Assembled Structures Composed of Amphipathic, Charged Tripeptides for Intracellular Delivery of Pro‐Apoptotic Chemotherapeutics

Self-assembled dipeptide based fluorescent nanoparticles as a platform for developing cellular imaging probes and targeted drug delivery chaperones

Preclinical Evaluation of Dimethyl Itaconate Against Hepatocellular Carcinoma via Activation of the e/iNOS-Mediated NF-κB–Dependent Apoptotic Pathway

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