Bispectra and System Identification

Papoulis, A.

doi:10.1007/978-94-009-0665-5_22

Cited by 1 publication

(2 citation statements)

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“…SPA is a computational approach that significantly enhances structural studies using microscopy. In SPA, particles are compared, and goodness-of-fit metrics such as cross-correlation [21,22] evaluate the particle before averaging. The typical procedure of SPA comprises (i) image acquisition, (ii) particle detection and segmentation, (iii) selection, (iv) alignment, (v) averaging and (vi) model generation.…”

Section: Single-particle Analysis Overcomes Critical Single-molecule ...mentioning

confidence: 99%

“…This usually involves calculating similarity metrics (e.g. cross-correlation at different relative rotations [21,22] for particle pairs and then sorting the particles into a suitable number of classes). Several SPA frameworks mentioned so far use classification to group particles according to their viewing orientation (e.g.…”

Section: Current Challenges In Single-particle Analysismentioning

confidence: 99%

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Mapping molecular complexes with super-resolution microscopy and single-particle analysis

et al. 2022

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Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM–SPA, demonstrating how it broadens our knowledge by elucidating features of key biological structures with unprecedented detail.

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Section: Single-particle Analysis Overcomes Critical Single-molecule ...mentioning

confidence: 99%

Section: Current Challenges In Single-particle Analysismentioning

confidence: 99%