Bivariate and trivariate analysis in flow cytometry: Phytoplankton size and fluorescence

Li, W. K. W.

doi:10.4319/lo.1990.35.6.1356

Cited by 16 publications

(5 citation statements)

References 11 publications

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“…Part of the background noise was eliminated by setting a threshold on the green fluorescence at 85 channels. As in most of the samples, HNA and LNA bacteria were clearly distinguishable by visual inspection of the cytogram of SSC versus FL1, the curve‐fitting methods (Li, 1990) were not used (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

A comparative study of the cytometric characteristics of High and Low nucleic‐acid bacterioplankton cells from different aquatic ecosystems

Bouvier

Giorgio

Gasol

2007

Environmental Microbiology

217

171

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Flow cytometry has revealed the existence of two distinct fractions of bacterioplankton cells, characterized by high and low nucleic acid contents (HNA and LNA cells). Although these fractions seem ubiquitous in aquatic systems, little is known concerning the variation in the cytometric parameters used to characterize them. We have performed cytometric analyses of samples from a wide range of aquatic systems to determine the magnitude and variability in the cytometric characteristics of HNA/LNA. We show that neither group is associated to a fixed level of fluorescence and of light scatter. Rather, the relative position of HNA and LNA in the fluorescence versus side scatter cytograms varies greatly, both within and among ecosystems. Although the cytometric parameters of both groups tend to covary, there is often uncoupling between the two, particularly in light scatter. Our results show that, although the basic HNA/LNA configuration is present in most samples, its cytometric expression changes greatly in different ecosystems and along productivity gradients. The patterns in cytometric parameters do not support the simple, dichotomous view of HNA and LNA as active and inactive cells, or the notion of two distinct and independent communities, but rather suggest that there may be cells that are intrinsic to each fraction, as well as others that may exchange between fractions.

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Section: Methodsmentioning

confidence: 99%

A comparative study of the cytometric characteristics of High and Low nucleic‐acid bacterioplankton cells from different aquatic ecosystems

Bouvier

Giorgio

Gasol

2007

Environmental Microbiology

217

171

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“…After mixing uniformly, place it statically in the dark for 15 min at room temperature, then determine it in the flowcytometer (FACSCalibur, Becton Dickinson Co.) to collect green fluorescence data (Marie et al, 1999). Its storing and processing methods are the same as that of photosynthetic picoplankton (Li, 1990).…”

Section: Analysis Of Bacmentioning

confidence: 99%

Community structure of picoplankton abundance and biomass in the southern Huanghai Sea during the spring and autumn of 2006

Ning

Liu

et al. 2010

Acta Oceanol. Sin.

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During spring and autumn of 2006, the investigations on abundance, carbon biomass and distribution of picoplankton were carried out in the southern Huanghai Sea (Yellow Sea, sHS). Three groups of picoplankton-Synechococcus (Syn), Picoeukaryotes (PEuk) and heterotrophic bacteria (BAC) were identified, but Prochlorococcus (Pro) was undetected. The average abundance of Syn and PEuk was lower in spring (5.0 and 1.3×10 3 cells/cm 3 , respectively) than in autumn (92.4 and 2.7×10 3 cells/cm 3 , respectively), but it was opposite for BAC (1.3 and 0.7×10 6 cells/cm 3 in spring and autumn, respectively). And the total carbon biomass of picoplankton was higher in spring (37.23±11.67) mg/m 3 than in autumn (21.29±13.75) mg/m 3 . The ratios of the three cell abundance were 5:1:1 341 and 30:1:124 in spring and autumn, respectively. And the ratios of carbon biomass of them were 5:7:362 and 9:4:4 in spring and autumn, respectively. Seasonal distribution characteristics of Syn, PEuk, BAC were quite different from each other. In spring, Syn abundance decreased in turn in the central waters (where phytoplankton bloom in spring occurred), the southern waters and inshore waters of the Shandong Peninsula (where even Syn was undetected); the high values of PEuk abundance appeared in the central and southern waters and the inshore of the Shandong Peninsula; the abundance of BAC was nearly three order of magnitude higher than that of photosynthetic picoplankton, and high values appeared in the central waters. In autumn, Syn abundance in central waters was higher than that in surrounding waters, while for PEuk abundance, it decreased in turn in the inshore waters of the Shandong Peninsula, the southern waters and the central waters; BAC presented a complicated blocky type distribution. Sub-surface maximum of each group of picopalnkton appeared in both spring and autumn. Compared with the available literatures concerning the studied area, the range of Syn abundance was larger, and the abundance of BAC was higher. In addition, the conversion factors for calculating picoplanktonic carbon biomass were discussed, with the conversion factors which are different from previous studies in the same surveyed waters. The result of regression analysis showed that there was distinct positive correlation between BAC and photosynthetic picoplankton in spring (r=0.61, P <0.001), but no correlation was found in autumn.

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“…Green fluorescence was collected in the FL1 channel ((530 ± 15) nm). After processing by CellQuest software, data were analyzed statistically using a double-variable diagram (Li 1990). …”

Section: Analysis Of Heterotrophic Bacteriamentioning

confidence: 99%

Distribution Pattern of Photosynthetic Picoplankton and Heterotrophic Bacteria in the Northern South China Sea

Cai

Ning

Liu

et al. 2007

JIPB

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The environmental regulation of picoplankton distribution in the northern South China Sea was examined in winter and summer of 2004. The average abundance of Synechococcus, Prochlorococcus, and heterotrophic bacteria was lower in winter (30, 21, and 780×10 3 cells/cm 3 , respectively) than in summer (53, 85, and 1 090×10 3 cells/cm 3 , respectively), but the seasonal pattern was opposite for picoeukaryotic phytoplankton (4 500 and 3 200 cells/cm 3 in winter and summer, respectively). Synechococcus, picoeukaryotes, and bacteria were most abundant in the nutrient-rich coastal zone and continental shelf, but Prochlorococcus was most abundant in the continental slope and open ocean. The vertical distribution of each photosynthetic group and heterotrophic bacteria changed between the two seasons. Synechococcus populations with apparently different phycoerythrobilin content occurred at many stations in the summer. In addition, two different populations of Prochlorococcus were found: (i) small, weakly fluorescing cells in the surface layer; and (ii) larger, strongly fluorescent cells in the deep layer. The distribution pattern of photosynthetic picoplankton and heterotrophic bacteria depends on environmental effects and their ecophysiological differences. The distribution of Synechococcus appeared to be related to nutrient availability, whereas the distribution of Prochlorococcus appeared to be limited by temperature. Synechococcus was the only picophytoplankton with a consistent strong relationship with bacteria.

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Bivariate and trivariate analysis in flow cytometry: Phytoplankton size and fluorescence

Cited by 16 publications

References 11 publications

A comparative study of the cytometric characteristics of High and Low nucleic‐acid bacterioplankton cells from different aquatic ecosystems

A comparative study of the cytometric characteristics of High and Low nucleic‐acid bacterioplankton cells from different aquatic ecosystems

Community structure of picoplankton abundance and biomass in the southern Huanghai Sea during the spring and autumn of 2006

Distribution Pattern of Photosynthetic Picoplankton and Heterotrophic Bacteria in the Northern South China Sea

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