Black corn (Zea mays L.) flour has the potential to improve the gut microbiota composition and goblet cell proliferation in mice fed a high-fat diet

Verediano, Thaísa Agrizzi; Sant'Ana, Cíntia Tomaz; Grancieri, Mariana; Tako, Elad; Paes, M. C. D.; Martino, Hércia Stampini Duarte

doi:10.1016/j.nutres.2022.10.005

Cited by 5 publications

(1 citation statement)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nutritional factors, such as irregular consumption of protein and casein peptides[ 3 ], may alter the dynamic regulation of intracellular occludin in TJs, specifically the permeability barrier to macromolecules[ 9 ]. A leaky gut facilitates the translocation of potentially harmful antigens into epithelial cells[ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Time-dependent impact of a high-fat diet on the intestinal barrier of male mice

Miranda,

Santana-Oliveira,

Vasques-Monteiro

et al. 2024

World J Methodol

View full text Add to dashboard Cite

BACKGROUND Excessive saturated fat intake compromises the integrity of the intestinal mucosa, leading to low-grade inflammation, impaired mucosal integrity, and increased intestinal permeability, resulting in the migration of lipopolysaccharide (LPS) to other tissues. AIM To evaluate the chronic effects (at 10 and 16 wk) of a high-fat diet (HFD) (with 50% energy as fat) on the phylogenetic gut microbiota distribution and intestinal barrier structure and protection in C57BL/6 mice. METHODS Forty adult male mice were divided into four nutritional groups, where the letters refer to the type of diet (control and HFD or HF) and the numbers refer to the period (in weeks) of diet administration: Control diet for 10 wk, HFD for 10 wk, control diet for 16 wk, and HFD for 16 wk. After sacrifice, biochemical, molecular, and stereological analyses were performed. RESULTS The HF groups were overweight, had gut dysbiosis, had a progressive decrease in occludin immunostaining, and had increased LPS concentrations. Dietary progression reduced the number of goblet cells per large intestine area and Mucin2 expression in the HF16 group, consistent with a completely disarranged intestinal ultrastructure after 16 wk of HFD intake. CONCLUSION Chronic HFD intake causes overweight, gut dysbiosis, and morphological and functional alterations of the intestinal barrier after 10 or 16 wk. Time-dependent reductions in goblet cell numerical density and mucus production have emerged as targets for countering obesity-driven intestinal damage.

show abstract

Section: Introductionmentioning

confidence: 99%