Black sugarcane decoction reduces rat brain ischemia

Handayani, Ety Sari; Nugraha, Zainuri Sabta; Nurmasitoh, Titis; Kuswati, Kuswati; Ahsani, Dwi Nur; Nanda, Ajeng G.

doi:10.18051/univmed.2016.v35.40-45

Cited by 2 publications

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“…The depletion of the bax protein expression on hippocampal part of the rats' brain with black sugarcane administration is also followed by the depletion of the volume of the brain ischemia as well. This result matches previous study, where the lower number of the brain ischemia was also found in the post-tBCCAOrats's brain that received boiled sugarcane water (4) . The observation of the ischemia volume of the rat brain is performed with TTC staining.…”

Section: Discussionsupporting

confidence: 92%

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Ethanol Extract of Black Sugarcane Decrease Ischemia Volume and Bax Expression In Rats’ Brain

Handayani

Nur

Kuswati

et al. 2020

Int. J. Hum. Health Sci.

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Background:Black sugarcane contains policosanol, quercetin antioxidant and 14-3-3 protein. These compounds are proven in a pre-clinical test of their ability to reduce brain ischemia. There are no pre-clinic test that prove the neuro-protectant act of black sugarcane extract on ischemia-induced rats, and how its mechanism to protect brain is not yet clear. This study’s purpose is to find the effect of ethanol extract of black sugarcane (BSEE) towards brains’ ischemic volume and to find is there any Bax role on the BSEE’s neuroprotectant mechanism.Methods: This study used fifteen male rats (Wistar). The rats were divided into three groups: group A that had been induced with BCCAO and received BSSE (group A), the rats that had been induced with BCCAO (group B), and sham group (group C). BSEE were given for a week, before bilateral ligation of common carotid artery. The BCCAO duration is 20 minutes, reperfusion periods of 2 hours. Rats’ head decapitation is done two hours after ligation. Brain tissues were colored with TTC. Ischemic brain area were analyzed using Image I software. Bax’ expression scoring was done in the brain preparations with immonohistochemical with Anti-Bax antibody. Anti-Bax proteins were anylized using ALLRED Score, while the statistic analyzing was done with One Way ANOVA.Results: There is a difference of ischemia volume’s mean (p=0,018) and bax protein expression (p=0,013) on the rats brain.Conclusion: BSEE is able to reduce ischemia volume and Bax protein expression on the rats’ brain.International Journal of Human and Health Sciences Vol. 04 No. 02 April’20 Page : 102-108

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Section: Discussionsupporting

confidence: 92%

“…The administration of 200 mg/ kgBBpolicosannol on stroke-induced Mongolian gerbils can lower the cerebral ischemic (3) . Previous studies had proven that boiled water from boiled black sugarcane can lower the ischemia volume of the rats' brain post-BCCAO (4) . The mechanism of the lowering of ischemia volume itself is not yet known.…”

Section: Introductionmentioning

confidence: 99%