Bladder Reconstruction with Human Amniotic Membrane in a Xenograft Rat Model: A Preclinical Study

Barski, Dimitri; Gerullis, Holger; Ecke, Thorsten; Yang, Jin; Varga, Gabriella; Boros, Mihály; Pintelon, Isabel; Timmermans, Jean‐Pierre; Otto, Thomas

doi:10.7150/ijms.18127

Cited by 24 publications

(32 citation statements)

References 38 publications

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“…Previously we have shown that hAM as a scaffold enables the development of tissue-engineered urothelium, which is in molecular and ultrastructural properties comparable to native urothelium (Jerman et al, 2014). Other studies have already used the hAM for bladder (Shakeri et al, 2008;Adamowicz et al, 2016;Barski et al, 2017) and urethral reconstruction (Shakeri et al, 2009;Wang et al, 2014) in animal models. Moreover, hAM was also used for reconstructive surgery of the ureteral obstruction in patients with extensive ureteral strictures (Koziak et al, 2007) and reconstructive surgery of strictured urethra (Koziak et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

Ramuta

Jerman

Tratnjek

et al. 2020

Front. Bioeng. Biotechnol.

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Section: Introductionmentioning

confidence: 99%

The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

Ramuta

Jerman

Tratnjek

et al. 2020

Front. Bioeng. Biotechnol.

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“…Previous studies on animals and humans showed good results in terms of urothelial re-epithelialization, but with an inadequate muscle regeneration because it was incomplete, disorganized, not well integrated into the new wall, and without adequate vascularization or innervation [ 7 ]. This fact determines the final bladder functionality [ 24 , 26 ]. Some studies have even performed a specific mechanical analysis to study the tensile strength of the new wall [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Use of an acellular collagen–elastin matrix to support bladder regeneration in a porcine model of peritoneocystoplasty

2018

Cent european J Uro

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IntroductionBladder reconstruction without using the intestine remains a challenge to this day despite the development of new biomaterials and cell cultures. Human bladder engineering is merely anecdotic, and mostly in vitro and animal studies have been conducted.Material and methodsIn our study using a porcine model, we performed a bladder augmentation using an autologous parietal peritoneum graft (peritoneocystoplasty) and determined whether the attachment of an acellular collagen-elastin matrix (Group 1) or lack of (Group 2) had better histologic and functional results. Thus far, peritoneocystoplasty has rarely been performed or combined with a biomaterial.ResultsAfter 6 weeks, we observed different degrees of retraction of the new bladder wall in both groups, although the retraction was lower and the histological analysis showed more signs of regeneration (neoangiogenesis and less fibrosis) in Group 1 than when compared with Group 2. No transitional cells were found in the new bladder wall in any of the groups, and no differences were observed in the functional test results.ConclusionsPerforming a peritoneocystoplasty is an easy and safe procedure. The data supports the benefit of an acellular collagen–elastin matrix to reinforce bladder regeneration. However, in our study we observed too much retraction of the new wall and the histologic results were not acceptable to consider it an appropriate cystoplasty technique.

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“…Semiquantitative scores of lymphocytes from 0 to 3 were used for inflammation. Inflammation of the ulcerated region was scored by counting lymphocytes in three fields (200× magnification) in three observer-randomized H&E slides (semiquantitative score: 0, < 5% cells/field; 1, 5–25%; 2, 25–50%; 3, > 50%) [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Novel approach to gastric mucosal defect repair using fresh amniotic membrane allograft in dogs (experimental study)

et al. 2017

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BackgroundGastric mucosal defect could result from several causative factors including the use of nonsteroidal anti-inflammatory drugs, Helicobacter pylori infection, gastrointestinal and spinal cord diseases, and neoplasia. This study was performed to achieve a novel simple, inexpensive, and effective surgical technique for the repair of gastric mucosal defect.MethodsSix adult male mongrel dogs were divided into two groups (three dogs each). In the control positive group (C + ve), dogs were subjected to surgical induction of gastric mucosal defect and then treated using traditional medicinal treatment for such a condition. In the amniotic membrane (AM) group, dogs were subjected to the same operation and then fresh AM allograft was applied. Clinical, endoscopic, biochemical (serum protein and lipid and pepsin activity in gastric juice), histopathological, and immunohistochemistry evaluations were performed.ResultsRegarding endoscopic examination, there was no sign of inflammatory reaction around the grafted area in the AM group compared to the C + ve group. The leukocytic infiltration in the gastric ulcer was well detected in the control group and was less observed in the AM group. In the AM group, the concentrations of both protein and lipid profiles were nearly the same as those in serum samples taken preoperatively at zero time, which indicated that the AM grafting acted the same as gastric mucosa. The re-epithelization of the gastric ulcer in the C + ve group was not yet detected at 21 days, while in the AM group it was well observed covering most of the gastric ulcer. AM accelerated the re-epithelization of the gastric ulcer. The fibrous connective tissue and the precursor of collagen (COL IA1) were poorly detected in the gastric ulcer with AM application.ConclusionUsing fresh AM allograft for repairing gastric mucosal defect in dogs showed great impact as a novel method to achieve optimum reconstruction of the gastric mucosal architecture and restoration of pre-epithelial, epithelial, and post-epithelial normal gastric mucosal barriers.

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Bladder Reconstruction with Human Amniotic Membrane in a Xenograft Rat Model: A Preclinical Study

Cited by 24 publications

References 38 publications

The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

Use of an acellular collagen–elastin matrix to support bladder regeneration in a porcine model of peritoneocystoplasty

Novel approach to gastric mucosal defect repair using fresh amniotic membrane allograft in dogs (experimental study)

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