Blastochloris tepida, sp. nov., a thermophilic species of the bacteriochlorophyll b-containing genus Blastochloris

Madigan, Michael T.; Resnick, Sol M.; Kempher, Megan L.; Dohnálková, Alice; Takaichi, Shinichi; Wang-Otomo, Zheng-Yu; Toyoda, Atsushi; Kurokawa, Ken; Mori, Hiroshi; Tsukatani, Yusuke

doi:10.1007/s00203-019-01701-4

Cited by 22 publications

(18 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…halochloris , growing optimally at 30–40 °C, pH 8.5, and 14–16% ( w / v ) salinity [ 5 ]. Both of these phototrophs assemble their light-harvesting machinery with bacteriochlorophyll (BChl) b [ 2 , 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ]; because of this, they are part of a distinct minority (< 5%) of known purple bacteria. The light-harvesting 1 (LH1) complexes of BChl b -containing purple bacteria exhibit Q y absorption bands at wavelengths beyond 1000 nm whose energy is much lower than that absorbed by BChl a -containing purple bacteria, whose LH1 complexes typically absorb near 880 nm [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…For BChl b -containing species, these include Blastochloris ( Blc. ) tepida, isolated from a hot spring in New Mexico (USA) [ 9 , 11 ], and Hlr. halochloris [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…The purple nonsulfur bacterium Blc. tepida grows optimally at 42 °C and up to 47 °C [ 9 , 11 ]. However, in contrast to Tch.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Salt- and pH-Dependent Thermal Stability of Photocomplexes from Extremophilic Bacteriochlorophyll b-Containing Halorhodospira Species

et al. 2022

Self Cite

View full text Add to dashboard Cite

Halorhodospira (Hlr.) species are the most halophilic and alkaliphilic of all purple bacteria. Hlr. halochloris exhibits the lowest LH1 Qy transition energy among phototrophic organisms and is the only known triply extremophilic anoxygenic phototroph, displaying a thermophilic, halophilic, and alkaliphilic phenotype. Recently, we reported that electrostatic charges are responsible for the unusual spectroscopic properties of the Hlr. halochloris LH1 complex. In the present work, we examined the effects of salt and pH on the spectroscopic properties and thermal stability of LH1-RCs from Hlr. halochloris compared with its mesophilic counterpart, Hlr. abdelmalekii. Experiments in which the photocomplexes were subjected to different levels of salt or variable pH revealed that the thermal stability of LH1-RCs from both species was largely retained in the presence of high salt concentrations and/or at alkaline pH but was markedly reduced by lowering the salt concentration and/or pH. Based on the amino acid sequences of LH1 polypeptides and their composition of acidic/basic residues and the Hofmeister series for cation/anion species, we discuss the importance of electrostatic charge in stabilizing the Hlr. halochloris LH1-RC complex to allow it to perform photosynthesis in its warm, hypersaline, and alkaline habitat.

show abstract

Section: Introductionmentioning

confidence: 99%

“…For BChl b -containing species, these include Blastochloris ( Blc. ) tepida, isolated from a hot spring in New Mexico (USA) [ 9 , 11 ], and Hlr. halochloris [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Salt- and pH-Dependent Thermal Stability of Photocomplexes from Extremophilic Bacteriochlorophyll b-Containing Halorhodospira Species

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…B lastochloris is a small genus of purple photosynthetic bacteria, distinguished by using bacteriochlorophyll b as its photosynthetic pigment and absorbing light near 1,000 nm as opposed to the bacteriochlorophyll a-containing purple bacteria, which absorb in the region of 860 nm (1). Currently, there are only four species recognized, Blastochloris viridis (2), B. sulfoviridis (3), B. gulmargensis (4), and B. tepida (5,6). They were originally placed in the genus Rhodopseudomonas but were transferred to the new genus, Blastochloris, by Hiraishi (7).…”

mentioning

confidence: 99%

“…B. viridis was the first photosynthetic species to have the structure of its reaction center solved (8), for which a Nobel Prize was awarded. The genome sequences for B. viridis and B. tepida had previously been determined (6,9).…”

mentioning

confidence: 99%

Genome Sequence of the Alphaproteobacterium Blastochloris sulfoviridis DSM 729, Which Requires Reduced Sulfur as a Growth Supplement and Contains Bacteriochlorophyll b

Kyndt

Salama

Meyer

2020

Microbiol Resour Announc

View full text Add to dashboard Cite

The genome sequence of Blastochloris sulfoviridis is 3.85 Mb with a GC content of 68%. Its nearest relative is B. tepida (average nucleotide identity [ANI], 91.5%), followed by B. viridis (ANI, 83%). According to ANI and whole-genome-based phylogenetic analysis, the nearest relatives of Blastochloris are Rhodoplanes and Rhodopseudomonas, confirming the recognition of distinct genera.

show abstract

Blastochloris

Imhoff¹

2020

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Blas.to.chlo'ris. Gr. masc. n. blastos bud shoot; Gr. masc. adj. chloros green; N.L. fem. n. Blastochloris green bud shoot. Proteobacteria / Alphaproteobacteria / Rhizobiales / Hyphomicrobiaceae / Blastochloris Blastochloris species are anoxygenic phototrophic Alphaproteobacteria that have bacteriochlorophyll b in their photosynthetic reaction centers. Crystals of the photosynthetic reaction centers of Blastochloris viridis were the first that have been studied in high‐resolution structure analysis at 3 Å resolution. Internal photosynthetic membranes are present as lamellae underlying and parallel to the cytoplasmic membrane. Cells are rod shaped to ovoid and exhibit polar growth, budding, and asymmetric cell division and form rosette‐like cell aggregates. They are motile by means of subpolar flagella and stain Gram‐negative. Straight‐chain monounsaturated C 18:1 is the predominant component of cellular fatty acids. Ubiquinones and menaquinones are present, and the lipopolysaccharides are characterized by a 2,3‐diamino‐2,3‐deoxy‐ d ‐glucose (DAG)‐containing, phosphate‐free lipid A with amide‐bound C 14:0 3OH . DNA G + C content (mol%) : 63.8–68.3. Type species : Blastochloris viridis (Drews and Giesbrecht 1966) Hiraishi 1997 ( Rhodopseudomonas viridis Drews and Giesbrecht 1966).

show abstract

Blastochloris tepida, sp. nov., a thermophilic species of the bacteriochlorophyll b-containing genus Blastochloris

Cited by 22 publications

References 35 publications

Salt- and pH-Dependent Thermal Stability of Photocomplexes from Extremophilic Bacteriochlorophyll b-Containing Halorhodospira Species

Salt- and pH-Dependent Thermal Stability of Photocomplexes from Extremophilic Bacteriochlorophyll b-Containing Halorhodospira Species

Genome Sequence of the Alphaproteobacterium Blastochloris sulfoviridis DSM 729, Which Requires Reduced Sulfur as a Growth Supplement and Contains Bacteriochlorophyll b

Blastochloris

Contact Info

Product

Resources

About