Blastocyst hatching in humans

Shafei, R. A.; Syrkasheva, A. G.; Romanov, Romanov A.Yu.; Макарова, Н. П.; Долгушина, Н. В.; Semenova, M. L.

doi:10.1134/s1062360417010106

Cited by 7 publications

(12 citation statements)

References 95 publications

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“…In primates, including humans, blastocyst hatching involves the zona rupture, driven by a hydrostatic pressure exerted by expanded blastocyst, causing a nick in the zona through which the blastocyst egresses out, leaving the empty zona largely intact. [14][15][16][17] This hatching behaviour is also observed in other mammalian species. 18,19 In contrast, a global zona dissolution and a complete disappearance of zona during blastocyst hatching occurs in hamsters and rabbits.…”

Section: Development Of Human Blastocysts Through Hatching and Implan...supporting

confidence: 77%

“…In the human, it is reported that the blastocyst hatching process is different in vivo compared to in vitro. 14,15,17 It is assumed that hatching of blastocysts, in vivo, occurs possibly at the abembryonic pole and implantation at the embryonic pole. 14,17 In contrast, hatching is reported to occur at the embryonic pole in IVF-cultured blastocysts.…”

Section: Development Of Human Blastocysts Through Hatching and Implan...mentioning

confidence: 99%

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Molecular regulators of human blastocyst development and hatching: Their significance in implantation and pregnancy outcome

Vani

Vasan

Adiga

et al. 2022

American J Rep Immunol

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In humans, blastocyst hatching and implantation events are two sequential, critically linked and rate‐limiting events for a prospective pregnancy. These events are regulated by embryo‐endometrium derived molecular factors which include hormones, growth factors, cytokines, immune‐modulators, cell adhesion molecules and proteases. Due to poor viability of blastocysts, they fail to hatch and implant, leading to a low ‘Live Birth Rates’, majorly contributing to infertility. Here, embryo‐derived biomarkers analysis plays a key role to assess potential biological viability of blastocysts which are capable of implantation and prospective pregnancy. Thus far, embryo‐derived biomarkers examined are mostly immune‐modulators which are thought to be associated with blastocyst development‐implantation and progression of pregnancy, leading to live births. There is an urgent need to develop a quantitative and a reliable non‐invasive approach aiding embryo selection for elective single embryo transfer and to minimize recurrent pregnancy loss and multiple pregnancies. In this article, we provide a comprehensive review on our current knowledge and understanding of potential embryo‐derived molecular regulators, that is, biomarkers, of development of human blastocysts, their hatching and implantation. We discuss their potential implications in the assessment of blastocyst implantation potential and pregnancy outcome in terms of live births in humans.

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Section: Development Of Human Blastocysts Through Hatching and Implan...supporting

confidence: 77%

Section: Development Of Human Blastocysts Through Hatching and Implan...mentioning

confidence: 99%

Molecular regulators of human blastocyst development and hatching: Their significance in implantation and pregnancy outcome

Vani

Vasan

Adiga

et al. 2022

American J Rep Immunol

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“…Mammalian blastocyst hatching is an exemplary earlydevelopment phenomenon preceding the implantation event, both being pre-requisites for subsequent embryo viability and successful pregnancy (Seshagiri et al 2009, Shafei et al 2017. It is well documented that the biological viability of in vitro cultured embryos is inferior to those developing in vivo (Harlow & Quinn 1982, Roth et al 1994, Stokes et al 2005, Chimote et al 2013.…”

Section: Introductionmentioning

confidence: 99%

“…Blastocysts of the mouse, similar to human, exhibit hatching behavior which includes expansion of blastocoel, its increased hydrostatic pressure, zona thinning and leading to rupture of the zona (Lin et al 2001, Sathananthan et al 2003, Leonavicius et al 2018. These mechanical events, occurring during hatching, are believed to be accompanied by secretory zonalytic protease-factors (Perona & Wassarman 1986, Seshagiri et al 2009, Shafei et al 2017). In the mouse, blastocyst-hatching enzymes such as (1) trypsinlike serine protease (Sawada et al 1990) and (2) a set of serine proteases ISP1 and ISP2 are implicated in blastocyst hatching (O'Sullivan et al 2001, Sharma et al 2006.…”

Section: Introductionmentioning

confidence: 99%

“…A plethora of implantation-associated molecules, such as steroid hormones, growth factors, cytokines, enzymes and transcription factors (Singh et al 2011, Bulun 2017, Raheem 2018, have been extensively studied. Ironically, they have not been examined, with any details, in the context of blastocyst hatching in species studied (Pan et al 2015, Shafei et al 2017, Zhan et al 2018. In mouse, embryonic expression of various cytokines was examined and their potential involvement in the regulation of development of preimplantation embryos has been reported (Gerwin et al 1995, Krussel et al 1998, Nakasato et al 2004, Robertson et al 2018.…”

Section: Introductionmentioning

confidence: 99%

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Expression of IL-1β and implantation serine proteases is required for mouse blastocyst hatching

et al. 2021

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Mammalian blastocyst hatching is critically an indispensable process for successful implantation. One of the major challenges in IVF clinics is to achieve superior embryonic development with intrinsically potent hatching-competent blastocyst. However, the molecular regulation of hatching phenomenon is poorly understood. In this study, we examined the expression and function of one of the cytokines, IL-1β during blastocyst hatching in the mouse. In particular, the expression of IL-1β (Interleukin-1β), IL-1ra (Interleukin-1 receptor antagonist) and their functional receptor IL-1rt1 (Interleukin 1 receptor type-1) in morulae, zona intact- and hatched- blastocysts was studied. Supplementation of IL-1β to cultured embryos accelerated blastocyst development with improved hatching (treated: 89.6 ± 3.6% vs untreated: 65.4 ± 4.1%). When embryos were treated with IL-1ra, blastocyst hatching was decreased (treated: 28.8 ± 3.1% vs untreated: 67.5 ± 3.8%). Moreover, IL-1β and IL-1ra influenced the expression of hatching enzymes viz., implantation serine proteases (ISP 1 and ISP 2). While IL-1β increased the embryonic mRNA expression of ISPs (ISP1: 2-4; ISP2: 9-11 fold), IL-1ra decreased expression. The protein localization studies revealed increased nuclear presence predominantly of ISP 2 in IL-1β treated blastocysts. This is the first report to show the functional significance of embryonic IL-1β in regulating hatching-associated proteases, particularly ISP2. These findings have implications in our understanding of molecular regulation of blastocyst hatching and implantation failure in other species including humans.

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A mechanical model of blastocyst hatching

Tvergaard

Needleman

2021

Extreme Mechanics Letters

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Blastocyst hatching in humans

Cited by 7 publications

References 95 publications

Molecular regulators of human blastocyst development and hatching: Their significance in implantation and pregnancy outcome

Molecular regulators of human blastocyst development and hatching: Their significance in implantation and pregnancy outcome

Expression of IL-1β and implantation serine proteases is required for mouse blastocyst hatching

A mechanical model of blastocyst hatching

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