Blastocysts can be rebiopsied for preimplantation genetic diagnosis and screening

Number of biopsied trophectoderm cells is likely to affect the implantation potential of blastocysts with poor trophectoderm quality

“…It is suggested that, for blastocysts with Grade B or C TE scores, 1-5 cells may be the appropriate biopsied TE cell number to maintain the implantation potential. Although fewer cells do not favor the diagnostic efficiency, it seems not to be a problem considering the high efficiency of re-examining the test-failure embryos in PGD cycles (32).…”

Impact of multiple blastocyst biopsy and vitrification-warming procedures on pregnancy outcomes

“…However, there is concern that removal of too many trophectoderm cells could be detrimental to an embryo's pregnancy potential (11), and very little information is available on outcomes of twicebiopsied blastocysts. Zhang et al (12) reported ten single euploid embryo transfers with twice biopsied and vitrifiedwarmed blastocysts, which resulted in five live births, although no control groups were presented. Likewise, Minasi and Greco (13) noted as unpublished data that they performed eight single-embryo transfers with the use of blastocysts biopsied twice but cryopreserved once, resulting in four live births.…”

Developmental potential of clinically discarded human embryos and associated chromosomal analysis

“…This technology can also be used to screen a wide variety of genetic disorders 12 13 . In ART clinics, SNP microarrays have been applied to genetic analyses of blastomeres from preimplantation embryos 14 15 16 and trophectoderm cells from blastocysts 17 mainly for the detection of aneuploidies of the 46 chromosomes.…”