Blastomyces dermatitidis: Stability studies on different yeast lysate antigens

Allison, Tiffany R.; Wright, J. L. W.; Scalarone, Gene M.

doi:10.4236/oji.2013.33014

Cited by 3 publications

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the past several years the thrust of research in our laboratory has been associated with studies on various strains of B. dermatitidis from human, animal, or environmental specimens from many geographical locations in an effort to better understand the immunobiology of the organism [18–27]. Diagnosis of the disease has presented major problems.…”

Section: Introductionmentioning

confidence: 99%

Blastomyces dermatitidisYeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis

Boyd

VanDyke

Scalarone

2013

Veterinary Medicine International

Self Cite

View full text Add to dashboard Cite

The systemic fungal infection, blastomycosis, which infects both humans and animals has presented a diagnostic challenge for clinicians for many years. The aim of this study was to evaluate the diagnostic sensitivity of Blastomyces dermatitidis yeast lysate antigens with respect to antibody detection in dogs with blastomycosis. Lysate antigens were prepared from B. dermatitidis isolates T-58 and T-66 (dogs, Tennessee) and WI-R and WI-J (dogs, Wisconsin). Based on results obtained from a preliminary comparative study, five combinations of these isolates and one individual isolate were tested against 92 serum specimens from dogs with culture-proven or histologically-confirmed blastomycosis, using the indirect enzyme-linked immunosorbent assay (ELISA). Mean absorbance values obtained from the sera ranged from 0.905 with the individual T-58 antigen to 1.760 using an antigen combination (T-58 + T-66 + WI-R). All of the 6 antigenic preparations were able to detect antibody in the serum specimens, but the antigen combinations detected antibody to a higher degree than the individual antigen. This study provides evidence that combinations of the yeast lysate reagents seem to be more efficacious for antibody detection in dog sera, but our laboratory is continuing to evaluate antigen lysate combinations for detection of antibodies in blastomycosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Blastomyces dermatitidisYeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis

Boyd

VanDyke

Scalarone

2013

Veterinary Medicine International

Self Cite

View full text Add to dashboard Cite

show abstract

Blastomyces dermatitidis: Antibody Detection in Sera from Dogs with Blastomycosis with Yeast Lysate Antigens Produced from Human and Dog Isolates

Mondada

Fullmer

Hungerford

et al. 2014

Veterinary Medicine International

View full text Add to dashboard Cite

Dogs are common hosts to the fungal organism Blastomyces dermatitidis, which causes the systemic disease blastomycosis. The goal of our study was to compare the reactivity of two B. dermatitidis yeast lysate antigens prepared from dog isolates (ERC-2, Wisconsin; T-58, Tennessee) and two lysate antigens prepared from human isolates (B5931 and B5896, Minnesota) against 48 serum specimens from dogs with confirmed blastomycosis using the indirect enzyme-linked immunosorbent assay (ELISA). Secondarily, we used three different ELISA substrates (Ultra TMB: A, SureBlue: B, and SureBlue Reserve: C) to compare the effectiveness of each substrate. Mean absorbance values ranged from 0.446 (B) to 0.651 (C) for the B5931 antigen and from 0.393 (B) to 0.540 (C) for the ERC-2 antigen in Trial 1. In Trial 2, the absorbance values ranged from 0.628 (B) to 0.909 (A) for the B5896 antigen and from 0.828 (B) to 1.375 (C) for the T-58 antigen. In Trial 1, the lysate antigen prepared from the human isolate B5931 exhibited the highest absorbance value and in Trial 2 the lysate prepared from the dog isolate T-58 was the most reactive. The overall results thus indicated that the T-58 lysate was the optimal reagent when used to detect antibody with the Sure-Blue Reserve substrate. Our laboratory is continuing to study B. dermatitidis antigen and substrate combinations for the reliable immunodiagnosis of blastomycosis in humans and animals.

show abstract

<i>Blastomyces dermatitidis</i> Antibody Responses in Serial Serum Specimens from Dogs with Blastomycosis: Comparison of Different Yeast Lysate Antigens

Shepherd¹,

Lutes²,

Scalarone³

2014

JBM

View full text Add to dashboard Cite

The systemic fungal organism, Blastomyces dermatitidis causes blastomycosis in animals and humans. This study was designed to evaluate antibody detection in 55 serial serum specimens from 9 dogs with blastomycosis using B. dermatitidis yeast lysate antigens produced from two human isolates (B5896; B5931) and two dog isolates (ERC-2; T-58) with the indirect enzyme linked immunosorbent assay (ELISA; peroxidase system) to determine an optimal lysate antigen(s) for use in the ELISA to detect antibody in the dog serum specimens. The mean absorbance values when the lysate antigens were compared with respect to their ability to detect antibody in the day 0 sera from the 9 dogs were 1.024 (ERC-2), 1.351 (B5896), 1.700 (B5931) and 2.084 (T-58) respectively. All of the reagents exhibited a high level of sensitivity and in all instances the amount of antibody declined as the time interval post-treatment increased, but the T-58 lysate prepared from the dog isolate from Tennessee was the optimal reagent. We continue to evaluate antigens for B. dermatitidis antibody detection in different immunodiagnostic assays.

show abstract

Blastomyces dermatitidis: Stability studies on different yeast lysate antigens

Cited by 3 publications

References 28 publications

Blastomyces dermatitidisYeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis

Blastomyces dermatitidisYeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis

Blastomyces dermatitidis: Antibody Detection in Sera from Dogs with Blastomycosis with Yeast Lysate Antigens Produced from Human and Dog Isolates

<i>Blastomyces dermatitidis</i> Antibody Responses in Serial Serum Specimens from Dogs with Blastomycosis: Comparison of Different Yeast Lysate Antigens

Contact Info

Product

Resources

About

Blastomyces dermatitidis: Stability studies on different yeast lysate antigens

Cited by 3 publications

References 28 publications

Blastomyces dermatitidisYeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis

Blastomyces dermatitidisYeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis

Blastomyces dermatitidis: Antibody Detection in Sera from Dogs with Blastomycosis with Yeast Lysate Antigens Produced from Human and Dog Isolates

&lt;i&gt;Blastomyces dermatitidis&lt;/i&gt; Antibody Responses in Serial Serum Specimens from Dogs with Blastomycosis: Comparison of Different Yeast Lysate Antigens

Contact Info

Product

Resources

About

<i>Blastomyces dermatitidis</i> Antibody Responses in Serial Serum Specimens from Dogs with Blastomycosis: Comparison of Different Yeast Lysate Antigens