Blastomycosis Detected by Microbial Cell-Free DNA in Renal Transplant Recipient

Bansal, Saurabh; Yadav, Manajyoti; Singhania, Namrata; Samal, Subhankar; Singhania, Girish

doi:10.1016/j.amjmed.2020.02.043

Cited by 11 publications

(22 citation statements)

References 2 publications

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“…10 New emerging tests such as microbial cell-free DNA testing can have quick turnaround time and help in diagnosing the disease sooner. 11,12 Unveiling of manifestations of histoplasmosis as a result of immune reconstitution inflammatory syndrome (IRIS) has been rarely reported in the literature. [5][6][7] Patients with active HIV infection who are not taking ART and have low CD4 count and high viral load have poor initial immune response to opportunistic infections.…”

Section: Discussionmentioning

confidence: 99%

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Histoplasma stomatitis unveiled: Not all opportunistic infections get better after initiation of antiretroviral therapy

Bansal

Singhania

Nukala

et al. 2021

Clinical Case Reports

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Section: Discussionmentioning

confidence: 99%

“…Antibody testing can have low sensitivity in immunocompromised patients 10 . New emerging tests such as microbial cell‐free DNA testing can have quick turnaround time and help in diagnosing the disease sooner 11,12 …”

Section: Discussionmentioning

confidence: 99%

Histoplasma stomatitis unveiled: Not all opportunistic infections get better after initiation of antiretroviral therapy

Bansal

Singhania

Nukala

et al. 2021

Clinical Case Reports

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“…Next‐generation sequencing creates full sequences from the free or fragmented DNA present in the plasma and compares it with stored DNA sequences in the database to find an exact match. Such technique can scan for the presence of DNA of multiple organisms, including bacterial, fungal, parasitic, and viral in one serum sample 12 . Shorter turnaround time when compared to traditional culture provides an additional benefit.…”

Section: Discussionmentioning

confidence: 99%

“…Such technique can scan for the presence of DNA of multiple organisms, including bacterial, fungal, parasitic, and viral in one serum sample. 12 Shorter turnaround time when compared to traditional culture provides an additional benefit. The test has a diagnostic sensitivity of 92.9% and can detect a potentially sepsis causing organism three times more often than initial blood cultures.…”

Section: Case Descriptionmentioning

confidence: 99%

Severe nonbacterial preseptal cellulitis from adenovirus detected via pooled meta‐genomic testing

Bansal

Nimmatoori

Singhania

et al. 2020

Clinical Case Reports

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Acute epibulbar infections are one of the most frequently diagnosed ophthalmologic infections. They are usually self-limiting but can lead to a viral preseptal cellulitis mimicking a severe bacterial infection. Early diagnosis is important to shorten the course of recovery. Preseptal cellulitis is a soft-tissue infection that develops secondary to trauma, coryza, or local skin inflammation. Infections are usually bacterial with hemophilus influenzae, staphylococci, and streptococci being the most common pathogens. 1,2 Although viruses are rarely implicated in the etiology, varicella is known to cause preseptal cellulitis via an eyelid infection without prior respiratory involvement. Rarely, adenovirus eye infections are complicated by bulbar cellulitis. Herein, we present a case of a young man with an adenovirus-associated preseptal cellulitis diagnosed by pooled meta-genomic testing and successfully treated with topical steroids.

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“…Lastly, publications were searched using specific fungal names and after manual examination five extra articles were added. Therefore, in total 52 articles were included in this review [ 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 ].…”

Section: Laboratory Diagnosis Of Fungal Infections By Ngsmentioning

confidence: 99%

Rapid Genomic Diagnosis of Fungal Infections in the Age of Next-Generation Sequencing

Tsang

Teng

Lau

et al. 2021

JoF

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Next-generation sequencing (NGS) technologies have recently developed beyond the research realm and started to mature into clinical applications. Here, we review the current use of NGS for laboratory diagnosis of fungal infections. Since the first reported case in 2014, >300 cases of fungal infections diagnosed by NGS were described. Pneumocystis jirovecii is the predominant fungus reported, constituting ~25% of the fungi detected. In ~12.5% of the cases, more than one fungus was detected by NGS. For P. jirovecii infections diagnosed by NGS, all 91 patients suffered from pneumonia and only 1 was HIV-positive. This is very different from the general epidemiology of P. jirovecii infections, of which HIV infection is the most important risk factor. The epidemiology of Talaromyces marneffei infection diagnosed by NGS is also different from its general epidemiology, in that only 3/11 patients were HIV-positive. The major advantage of using NGS for laboratory diagnosis is that it can pick up all pathogens, particularly when initial microbiological investigations are unfruitful. When the cost of NGS is further reduced, expertise more widely available and other obstacles overcome, NGS would be a useful tool for laboratory diagnosis of fungal infections, particularly for difficult-to-grow fungi and cases with low fungal loads.

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Blastomycosis Detected by Microbial Cell-Free DNA in Renal Transplant Recipient

Cited by 11 publications

References 2 publications

Histoplasma stomatitis unveiled: Not all opportunistic infections get better after initiation of antiretroviral therapy

Histoplasma stomatitis unveiled: Not all opportunistic infections get better after initiation of antiretroviral therapy

Severe nonbacterial preseptal cellulitis from adenovirus detected via pooled meta‐genomic testing

Rapid Genomic Diagnosis of Fungal Infections in the Age of Next-Generation Sequencing

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