Blimp-1 is involved in B cell activation and maturation in Nile tilapia (Oreochromis niloticus)

Wu, Liting; Zhou, Enxu; Gao, Along; Kong, Linghe; Wu, Siwei; Bian, Xia; Li, Yuan; Li, Bingxi; Fu, Shengli; Guo, Zheng; Ye, Jianmin

doi:10.1016/j.dci.2019.05.002

Cited by 26 publications

(16 citation statements)

References 56 publications

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“…Different studies have identified prdm1 homolog genes in several teleost fish species such as zebrafish Danio rerio (28,29), fugu Takifugu rubripes (30), medaka Oryzias latipes (31), Japanese flounder Paralichthys olivaceus (32), or Nile tilapia Oreachromis niloticus (33). In medaka, two different genes have been described, prdm1a and prdm1b, both of which show detectable expression during embryo development and in all the tissues including immune organs (31).…”

Section: Introductionmentioning

confidence: 99%

Insights Into the Evolution of the prdm1/Blimp1 Gene Family in Teleost Fish

Perdiguero¹,

Goméz-Esparza²,

Martin³

et al. 2020

Front. Immunol.

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In mammals, Blimp1 (B lymphocyte-induced maturation protein 1) encoded by the prdm1 gene and its homolog Hobit (homolog of Blimp1 in T cells) encoded by znf683, represent key transcriptional factors that control the development and differentiation of both B and T cells. Despite their essential role in the regulation of acquired immunity, this gene family has been largely unexplored in teleosts to date. Until now, one prdm1 gene has been identified in most teleost species, whereas a znf683 homolog has not yet been reported in any of these species. Focusing our analysis on rainbow trout (Oncorhynchus mykiss), an in silico identification and characterization of prdm1-like genes has been undertaken, confirming that prdm1 and znf683 evolved from a common ancestor gene, acquiring three gene copies after the teleost-specific whole genome duplication event (WGD) and six genes after the salmonid-specific WGD. Additional transcriptional studies to study how each of these genes are regulated in homeostasis, in response to a viral infection or in B cells in different differentiation stages, provide novel insights as to how this gene family evolved and how their encoded products might be implicated in the lymphocyte differentiation process in teleosts.

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Section: Introductionmentioning

confidence: 99%

Insights Into the Evolution of the prdm1/Blimp1 Gene Family in Teleost Fish

Perdiguero¹,

Goméz-Esparza²,

Martin³

et al. 2020

Front. Immunol.

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“…Pax5 is expressed from pre-B cells to mature B cells, while Blimp1 is a master regulator of terminal B cells by down-regulating the expression of Pax5 and leading to the maturation of plasmablasts to plasma cells (92). Significantly higher expressions of Pax5 and Blimp1 have been demonstrated in resting IgM + B cells than in IgM − cells (71,97,98). However, Pax5 is usually suppressed by its repressor, Blimp1, in activating B cells, leading to shifts in the expression of immunoglobulin from membrane to secreted forms (99).…”

Section: B-cell Differentiation and Phagocytic Capacitymentioning

confidence: 99%

Recent Advances on Phagocytic B Cells in Teleost Fish

Qin

Liu

et al. 2020

Front. Immunol.

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The momentous discovery of phagocytic activity in teleost B cells has caused a dramatic paradigm shift from the belief that phagocytosis is performed mainly by professional phagocytes derived from common myeloid progenitor cells, such as macrophages/monocytes, neutrophils, and dendritic cells. Recent advances on phagocytic B cells and their microbicidal ability in teleost fish position B cells at the crossroads, bridging innate with adaptive immunity. Most importantly, an increasing body of experimental evidence demonstrates that, in both teleosts and mammals, phagocytic B cells can recognize, take up, and destroy particulate antigens and then present those processed antigens to CD4 + T cells to elicit adaptive immune responses and that the phagocytosis is mediated by pattern recognition receptors and involves multiple cytokines. Thus, current findings collectively indicate that teleost phagocytic B cells, as well as their counterpart mammalian B1-B cells, can be considered one kind of professional phagocyte. The aim of this review is to summarize recent advances regarding teleost phagocytic B cells, with a particular focus on the recognizing receptors and modulating mechanisms of phagocytic B cells and the process of antigen presentation for T-cell activation. We also attempt to provide new insights into the adaptive evolution of the teleost fish phagocytic B cell on the basis of its innate and adaptive roles.

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“…The total volume 10 mL of single-cell suspension was layered upon the same volume of Histopaque 1077 (Sigma, USA) in 50 mL centrifuge tubes gradually, centrifuged 500 x g for 40 min at 4 ℃. Leukocytes were collected from the interface layer and washed three times with medium [30,31]. Cell quantity and viability were determined by 0.4% trypan blue (Sigma, USA), and cells were collected for qPCR analysis [32].…”

Section: Basal Expression Of Immunoglobulin Isotypes In Isolated Leukmentioning

confidence: 99%

Molecular Characterization of A New IgZ3 Subclass from Common Carp (Cyprinus Carpio) and Comparative Expression Analysis of IgH Transcripts During Ontogeny of Larvae

Zhang

et al. 2020

Preprint

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Background Immunoglobulins distributing among systemic immune tissues and mucosal immune tissues play an important role in teleost to protect them from infections in a pathogen-rich aquatic environment. Teleost IgZ/IgT subclasses with different tissue expression patterns may have varied immune functions.Results In the present study, a novel secreted IgZ heavy chain gene was cloned and characterized in common carp (Cyprinus carpio) which was different from the reported IgZ1 and IgZ2 in tissue-specific expression profile. The obtained IgZ-like subclass was designated as CcIgZ3 which complete open reading frame contained 1650 bp encoding a protein of 549 amino acid residues. The phylogenetic analysis revealed that CcIgZ3 was grouped with carp IgZ2 and was in the same branch as other teleosts IgZ/IgT. Basal expression detection of IgH in healthy adult common carp showed that CcIgZ3 transcripts were widely expressed in systemic immune tissues and Mucosal-associated lymphoid tissues. It was expressed at a higher level in the head kidney, gill, and gonad, followed by spleen, hindgut, oral epithelium, liver, brain, muscle, foregut, and blood, but at a very low level in the skin. The transcript level of CcIgZ3 mRNA in isolated leukocytes from peripheral blood cells was significantly higher than that of isolated leukocytes from the spleen. Different groups of common carp were infected with Aeromonas hydrophila via intraperitoneal injection and immersion respectively. The qRT-PCR analysis demonstrated that a significant difference in CcIgZ3 mRNA level between immersion and injection groups existed in all the detected tissues including head kidney, spleen, liver, and hindgut, especially in hindgut CcIgZ3 mRNA level was higher in immersion than in the injection group. Different routes for Aeromonas hydrophila challenge to common carp had comparatively less effect on IgM response. Further study on the relative expression of the IgH gene during the ontogeny of common carp presented that the tissue-specific expression profile of CcIgZ3 was very different from the others. In the early larval development stage of common carp from 1 dpf to 31 dpf, the qRT-PCR analysis demonstrated that the CcIgZ3 mRNA level increased gradually with a similar dynamic tendency of IgZ1 and IgZ2 and IgM was the dominant Ig with obviously higher abundance. The results of tissue-specific expression of IgH at 65 dpf of common carp showed that CcIgZ3 was expressed at mucosal sites including both hindgut and gill but IgZ1 was preferentially expressed in hindgut and IgZ2 in gill. Except for qRT-PCR analysis, the detection of the CcIgZ3-expressing cells and IgM-expressing cells by in situ hybridization was performed. The results showed that CcIgZ3 and IgM transcripts could be detected in the spleen, gill, and hindgut of the common carp at 65dpf. Conclusions These results revealed that CcIgZ3 gene transcript occurred in the early development stage of common carp not only in systemic tissues but also in mucosal tissues. CcIgZ3 could be induced with significantly different expression profiles at immune tissues after the challenge by Aeromonas hydrophila immersion and intraperitoneal injection, which indicated that CcIgZ3 might play a more important role in mucosal immunity than in systemic immunity.

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Blimp-1 is involved in B cell activation and maturation in Nile tilapia (Oreochromis niloticus)

Cited by 26 publications

References 56 publications

Insights Into the Evolution of the prdm1/Blimp1 Gene Family in Teleost Fish

Insights Into the Evolution of the prdm1/Blimp1 Gene Family in Teleost Fish

Recent Advances on Phagocytic B Cells in Teleost Fish

Molecular Characterization of A New IgZ3 Subclass from Common Carp (Cyprinus Carpio) and Comparative Expression Analysis of IgH Transcripts During Ontogeny of Larvae

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