Blood and urine physiological values in farm-cultured <i>Rana catesbeiana</i> (Anura: Ranidae) in Argentina

Abstract

A total of 302 samples of healthy farm-cultured Rana catesbeiana specimens (9-21 months-old, 50-350 g liveweight, 50% each sex) from the northeast of Argentina, were analyzed through spectrophotometry, electrophoresis, densitometry, refractometry and microscopy in order to obtain blood and urine normal values. Confidence intervals (p<0.05) for PCV (28.6-31.6%), RBC (0.40-0.44 T/L), MCV (686-732 fL), hemoglobin (6.41-7.20 g/dL), MCH (151-164 pg), MCHC (22.6-24.0%), WBC (18.7-22.3 G/L), neutrophils (58.4-63.4%), lymphocytes (23.9-29.8%), monocytes (2.1-3.8%), eosinophils (4.6-7.0%), basophils (2.9-4.1%), bleeding time (289-393s), coagulation time (452-696s), prothrombin time (76-128s), urinary density (1.0061-1.0089 g/mL), urinary pH (6,38-6.96), fibrinogen (0.59-0.99 g/dL), total protein (4.19-4.49 g/dL), albumin (1.49-1.67 g/dL), alpha-1 globulin (0.20-0.24 g/dL), alpha-2 globulin (0.48-0.54 g/dL), beta globulin (0.68-0.77 g/dL), gamma globulin (1.28-1.42 g/dL), albumin/globulin ratio (0.50-0.58), creatinine (4.09-5.56 mg/L), urea (76.1-92.4 mg/L), uric acid (11.5-15.4 mg/L), triglycerides (0.34-0.52 g/L), total cholesterol (0.56-0.67 g/L), HDL-C (0.03-0.05 g/L), LDL-C (0.34-0.44 g/L), alpha lipoprotein (6.01-8.67%), beta lipoprotein (91.3-93.9%), glucose (0.45-0.54 g/L), Na (116-121 meq/L),

Colouration in amphibians as a reflection of nutritional status: The case of tree frogs in Costa Rica

“…Glucose values found in this study were similar to those reported for Rana catesbeiana in captivity and free-range Polypedates teraiensis (38–53 mg/dl) [33,41,63,64], although A . callidryas from the Pacific showed slightly higher values.…”

“…Haematocrit values of all groups were similar to those found for several anurans, within a range of 22.4 to 58.5% [33,43,64,66,67], which is indicative of adequate hydration status. Changes in haematocrit have been shown as a consequence of the alimentary status of the animal, the supply of specific minerals (iron, copper and selenium) in addition to dietary protein, folic acid, cobalamin and niacin.…”

“…catesbeiana in captivity [63,64], Polypedates teraiensis [33] and free-range Pseudepidalea viridis , Pelobates syriacus , Rana dalmatina , Hyla arborea and Pelophylax ridibundus [43]. In carnivores, glucogenic aminoacids can influence glucose synthetized from gluconeogenesis and its utilization [36].…”

“…Changes in haematocrit have been shown as a consequence of the alimentary status of the animal, the supply of specific minerals (iron, copper and selenium) in addition to dietary protein, folic acid, cobalamin and niacin. In various studies, haematocrit has been shown to be affected by environmental conditions as well as the affinity for oxygen by the haemoglobin (oxidative status), depending on the species [36,40,63,64]. A .…”

Hematology of Wild Caught Dubois’s Tree FrogPolypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)

“…Formulae used by Arserim and Mermer [14] were followed for measurement of blood cells. For the other hematological investigations, procedures described by Coppo et al [15] were followed. …”