BLOOD COMPONENTS: Red blood cell hemolysis during blood bank storage: using national quality management data to answer basic scientific questions

Hess, John R.; Meer, Pieter F. van der; Acker, Jason P.; Cardigan, Rebecca; Devine, Dana V.

doi:10.1111/j.1537-2995.2009.02275.x

Cited by 151 publications

(165 citation statements)

References 18 publications

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“…Each 0.1 unit of pH change results in a 0.4 mmol/L change in the serum potassium level. Potassium levels are increased by acidosis and decreased by alkalosis [13]. So, this also leads to hyperkalemia.…”

Section: Discussionmentioning

confidence: 99%

Effect of Blood Storage on Complete Biochemistry

Verma¹,

Dahiya²

2015

J Blood Disord Transfus

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Section: Discussionmentioning

confidence: 99%

Effect of Blood Storage on Complete Biochemistry

Verma¹,

Dahiya²

2015

J Blood Disord Transfus

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“…Reversible and irreversible changes to the function and properties of RBCs can be observed as early as 14 days after collection (Table 4) [36]. Stored units of blood experience progressive biochemical and morphological deterioration resulting in RBCs that have compromised viability, cell size, deformability, and lipid-membrane composition (Table 4) [7,8,34,37,38].…”

Section: Storage Lesionmentioning

confidence: 99%

Storage of Red Blood Cells and Transfusion-Related Acute Lung Injury

Babaev

Pozzi

Hare

et al. 2014

JACCOA

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Transfusion-related acute lung injury (TRALI) is a major complication posttransfusion. A consensus definition of TRALI has been recently established to improve diagnosis but the pathogenesis of TRALI is yet to be understood. Although the antibody-mediated two-hit model of TRALI is the classical narrative, increasing evidence of the probable implications of prolonged storage of blood provides novel mechanisms towards storage lesion-the potentially injurious cellular and biochemical changes that occur in stored red blood cells. Red blood cell-derived lipids and micro vesicles may have been playing an important role in the development of TRALI. This article will provide a brief overview of the current understanding of TRALI and then discuss the implications and the potential mechanisms by which stored red blood cells may lead to TRALI. TRALI is characterized by clinical features that include, but not limited to, dyspnea, tachypnea, respiratory insufficiency, noncardiogenic bilateral pulmonary edema, decreased pulmonary compliance and acute hypoxemia (PaO 2 /FiO 2 <300 mmHg, (Table 1) [13,14]. KeywordsThe incidence of TRALI ranges from 1/5,000-1/1,323 transfusions within the general population and it is the leading cause of transfusion-related death in the United States [15]. Such large range in incidence could be attributed to the relatively unspecific diagnostics tools available for TRALI, past cases of under-diagnosis or misdiagnosis, and the elevated incidence of TRALI within vulnerable populations [14][15][16]. A recent study analyzing the incidence of TRALI in critically ill patients admitted to intensive care unit (ICU) observed that over 5% of all patients who received blood transfusion developed TRALI, which is 50-100 times greater occurrence than in the general hospital population [12,17]. Risk factors related to recipients and donorsThe risk factors of TRALI are highly diverse, among patients DefinitionDiagnostic Parameters TRALIBi-lateral infiltrates with non-cardiogenic pulmonary edema. ARDS appearance within 6hours after transfusion. Hypoxemia: PaO 2 /F i O 2 < 300 mmHg. Potential TRALI Similar to TRALI diagnosis with an additional risk-factor for lung injury/ARDS. Delayed TRALI Diagnostic parameters characteristic of TRALI within 24-72 hours after transfusion.

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“…At concentrations up to 3.125 mmol/l, Trolox was somewhat more effective than mannitol in protecting against progressive hemolysis. However, the percentage of post-storage hemolysis might strongly depend on mannitol supplementation [16]. Importantly, concentrations of mannitol in commercial AS are over 10-fold higher than those used in our experiments.…”

Section: Discussionmentioning

confidence: 87%

Vitamin E Analogue Protects Red Blood Cells against Storage-Induced Oxidative Damage

Antosik

Czubak

Cichoń

et al. 2018

Transfus Med Hemother

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Background: To investigate i) the effects of Trolox® or mannitol, which represent two different classes of antioxidants, on oxidative changes generated in manually isolated red blood cells (RBCs) from citrate-phosphate-dextrose (CPD) preserved whole blood, followed by up to 20 days refrigerated storage, and ii) whether Trolox supplemented to the blood bank-manufactured saline-adenine-glucose-mannitol (SAGM) preserved RBC units would offer better storage conditions compared with SAGM alone. Methods: The percentage of hemolysis and extracellular activity of lactate dehydrogenase (LDH) was measured to assess RBC membrane integrity. Lipid peroxidation, reduced glutathione (GSH) levels and total antioxidant capacity (TAC) were quantified by thiobarbituric acid-reactive substances (TBARS), Ellman's reagent and 2, 2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS.+) based assay, respectively. Results: Trolox was little more effective than mannitol in protecting against progressive RBC hemolysis. Trolox (0.125-3.125 mmol/l) inhibited storage-induced leakage of LDH, lipid peroxidation, and to a lesser extent GSH depletion. Mannitol at these concentrations neither inhibited TBARS formation nor prevented GSH depletion. RBC units stored in SAGM-Trolox had significantly lower hemolysis, LDH leakage, and lipid peroxidation level compared to RBCs stored in SAGM. Conclusion: There is evidence of the beneficial effects of supplementing RBC-additive solutions with membrane-interacting antioxidants such as vitamin E analogues.

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BLOOD COMPONENTS: Red blood cell hemolysis during blood bank storage: using national quality management data to answer basic scientific questions

Abstract: Large national data sets provide useful information about the distribution of hemolysis at the end of RBC storage. This information can aid blood storage system development and regulatory science.

Cited by 151 publications

References 18 publications

Effect of Blood Storage on Complete Biochemistry

Effect of Blood Storage on Complete Biochemistry

Storage of Red Blood Cells and Transfusion-Related Acute Lung Injury

Vitamin E Analogue Protects Red Blood Cells against Storage-Induced Oxidative Damage

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