Blood-Derived Human iPS Cells Generate Optic Vesicle–Like Structures with the Capacity to Form Retinal Laminae and Develop Synapses

Phillips, M. Joseph; Wallace, Kyle; Dickerson, Sarah; Miller, M.; Verhoeven, Amelia D.; Martin, Jessica; Wright, Lynda S.; Shen, Wei; Capowski, Elizabeth E.; Perçin, Ferda; Perez, Enio T.; Zhong, Xiufeng; Canto‐Soler, M. Valeria; Gamm, David M.

doi:10.1167/iovs.11-9313

Cited by 191 publications

(192 citation statements)

References 37 publications

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“…However, the production of photoreceptors under these conditions is relatively low. By contrast, protocols that maintain cells as embryoid bodies for extended periods allow for the development of highly organized, laminated retina-like structures in vitro (Eiraku et al, 2011;Lamba and Reh, 2011;Nakano et al, 2012;Phillips et al, 2012;Zhong et al, 2014). In these structures, the percentage of photoreceptors that develop is much higher than in dissociated cultures, suggesting that tissue organization is important for photoreceptor specification.…”

Section: Discussionmentioning

confidence: 99%

Photoreceptor cell fate specification in vertebrates

2015

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Photoreceptors -the light-sensitive cells in the vertebrate retinahave been extremely well-characterized with regards to their biochemistry, cell biology and physiology. They therefore provide an excellent model for exploring the factors and mechanisms that drive neural progenitors into a differentiated cell fate in the nervous system. As a result, great progress in understanding the transcriptional network that controls photoreceptor specification and differentiation has been made over the last 20 years. This progress has also enabled the production of photoreceptors from pluripotent stem cells, thereby aiding the development of regenerative medical approaches to eye disease. In this Review, we outline the signaling and transcription factors that drive vertebrate photoreceptor development and discuss how these function together in gene regulatory networks to control photoreceptor cell fate specification.

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Section: Discussionmentioning

confidence: 99%

Photoreceptor cell fate specification in vertebrates

2015

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“…Importantly, for such applications, an efficient cryopreservation method has been also invented to store these human ES cell-derived 3D NR tissue (Nakano et al, 2012). The layer-forming nature of NR derived from human induced pluripotent stem (iPS) cells was also reported in a recent independent study (Phillips et al, 2012), supporting the reliability and robustness of this strategy.…”

Section: Retinal Development In Vivomentioning

confidence: 92%

In vitro organogenesis in three dimensions: self-organising stem cells

2012

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SummaryOrgan formation during embryogenesis is a complex process that involves various local cell-cell interactions at the molecular and mechanical levels. Despite this complexity, organogenesis can be modelled in vitro. In this article, we focus on two recent examples in which embryonic stem cells can self-organise into three-dimensional structures -the optic cup and the pituitary epithelium; and one case of self-organising adult stem cells -the gut epithelium. We summarise how these approaches have revealed intrinsic programs that drive locally autonomous modes of organogenesis and homeostasis. We also attempt to interpret the results of previous in vivo studies of retinal development in light of the self-organising nature of the retina.

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“…These 3D functional organoids have improvised our understanding of early human development and improved drug screening methodologies organoids including mini gut, mini heart (repopulating decellularized mice heart through iPS), mini brain (developing cortex, central telencephalon and choroid plexus), small lungs, mini eyes (optic vesicle like structures) have been formulated for disease remodeling purposes [82][83][84][85][86][87][88][89].…”

Section: D Organoidsmentioning

confidence: 99%

Ips Progression a Decade Devoted

K¹

2017

J Cell Sci Ther

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Yamanaka and Takahashi's astonishing discovery in 2006 revolutionized the world of stem cells. Simplicity and reproducibility of IPSC's cells opened doors for extensive therapeutic advancements and potential clinical trials particularly in the field of Regenerative medicine. In 2012, nobel prize was presented to both researchers for the breakthrough in reprogramming somatic cells to a pluripotent state with the expression of "Yamanakas cocktail" of OKSM quartet transcription factors: sex determining region y box2 (sox2), octamer binding transcription factor4 (oct4), krupple like factor (klf4) and myelocymatosis oncogene (c-myc). Rationale of iPS use is attributed to its unlimited cell source circumventing ethical hindrance. This comprehensive review will summarize mechanisms of IPS production and cell therapy applications. Moreover, appreciate the improvements and initiatives in iPS technology, scrutinize the scientific claim of indistinguishable resemblance of iPS and eSC, evaluate the constraints during iPS manipulation and analyze safety of these pluripotent cells in clinical scenarios.

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Blood-Derived Human iPS Cells Generate Optic Vesicle–Like Structures with the Capacity to Form Retinal Laminae and Develop Synapses

Cited by 191 publications

References 37 publications

Photoreceptor cell fate specification in vertebrates

Photoreceptor cell fate specification in vertebrates

In vitro organogenesis in three dimensions: self-organising stem cells

Ips Progression a Decade Devoted

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