Blood Gene Expression Profile Study Revealed the Activation of Apoptosis and p53 Signaling Pathway May Be the Potential Molecular Mechanisms of Ionizing Radiation Damage and Radiation-Induced Bystander Effects

He, Guobin; Tang, Anzhou; Xie, Mao; Xia, Wei; Zhao, Pengcheng; Wei, Jianglian; Lai, Yongjing; Tang, Xianglong; Zou, Yi Ming; Liu, Heng

doi:10.1177/1559325820914184

Cited by 9 publications

(8 citation statements)

References 49 publications

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“…However, the sensitivity of spermatogenic populations to RIAEs is unclear. Previous studies have demonstrated that RIAEs can initiate apoptosis in distant tissues ( Koturbash et al, 2008 ; He et al, 2020 ). In addition, Zhang et al reported that fractionated irradiation (X-ray, 8Gy×3 d) of the right thorax damaged the ultrastructure of the BTB and increased apoptotic spermatogonia cells, which located at the outermost layer of the seminiferous epithelium of the testis ( Zhang et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism of testicular cell apoptosis directly induced by ionising radiation is mostly mediated by a p53-dependent Bax-caspase-3-mediated pathway ( Shahin et al, 2018 ; He et al, 2020 ). Since the testes of mice in the C-irradiation group are not directly exposed to ionising radiation, we speculate that the mechanism of testicular cell apoptosis induced by C-irradiation is different from that induced by direct radiation.…”

Section: Discussionmentioning

confidence: 99%

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The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

et al. 2021

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To investigate whether the abscopal effects of cranial irradiation (C-irradiation) cause testicular damage in mice, male C57BL/6 mice (9weeks of age) were randomly divided into a sham irradiation group, a shielded group and a C-irradiation group and administered sham/shielded irradiation or C-irradiation at a dose rate of 2.33Gy/min (5Gy/d for 4 d consecutively). All mice were sacrificed at 4weeks after C-irradiation. We calculated the testis index, observed testicular histology by haematoxylin-eosin (HE) staining and observed testicular ultrastructure by transmission electron microscopy. Western blotting was used to determine the protein levels of Bax, Bcl-2, Cleaved caspase 3, glial cell line-derived neurotrophic factor (GDNF) and stem cell factor (SCF) in the testes of mice. Immunofluorescence staining was performed to detect the expression of Cleaved caspase 3 and 3β hydroxysteroid dehydrogenase (3βHSD), and a TUNEL assay was used to confirm the location of apoptotic cells. The levels of testosterone (T), GDNF and SCF were measured by ELISA. We also evaluated the sperm quality in the cauda epididymides by measuring the sperm count, abnormality, survival rate and apoptosis rate. The results showed that there was no significant difference in testicular histology, ultrastructure or sperm quality between the shielded group and sham group. Compared with the sham/shielded group, the C-irradiation group exhibited a lower testis index and severely damaged testicular histology and ultrastructure at 4weeks after C-irradiation. The levels of apoptosis in the testes increased markedly in the C-irradiation group, especially in spermatogonial stem cells. The levels of serum T and testicular 3βHSD did not obviously differ between the sham group and the C-irradiation group, but the levels of GDNF and SCF in the testes increased in the C-irradiation group, compared with the sham group. In addition, the sperm count and survival rate decreased in the C-irradiation group, while the abnormality and apoptosis rate increased. Under these experimental conditions, the abscopal effects of C-irradiation induced testicular damage with regard to both structure and function and ultimately decreased sperm quality in mice. These findings provide novel insights into prevention and treatment targets for male reproductive damage induced by C-irradiation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

et al. 2021

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“…While RT is known to affect the tumor microenvironment, including tumor blood vessels and immune system cells ( 16 – 19 ), how it triggers systemic bystander effects is unclear. Previous studies have implicated apoptosis and p53 signaling in such effects ( 20 ). Through preliminary analysis of single-cell data, we provide evidence that the immune microenvironment of tumors varies during irradiation, and that radiation-specific CD8+ T cells may be the primary risk factor for RT-associated adverse reactions.…”

Section: Discussionmentioning

confidence: 99%

Single-cell mechanistic studies of radiation-mediated bystander effects

Han

Chen²,

Zhang³

et al. 2022

Front. Immunol.

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Ionizing radiation (IR) has been widely used in the diagnosis and treatment of clinical diseases, with radiation therapy (RT) being particularly rapid, but it can induce “bystander effects” that lead to biological responses in non-target cells after their neighboring cells have been irradiated. To help clarify how radiotherapy induces these effects, To help clarify how radiotherapy induces these effects, we analyzed single-cell RNA sequencing data from irradiated intestinal tissues on day 1 (T1 state), day 3 (T3 state), day 7 (T7 state), and day 14 (T14 state) after irradiation, as well as from healthy intestinal tissues (T0 state), to reveal the cellular level, molecular level, and involvement of different time irradiated mouse intestinal tissues in biological signaling pathways. In addition, changes in immune cell subpopulations and myeloid cell subpopulations after different radiation times were further explored, and gene regulatory networks (GRNs) of these cell subpopulations were constructed. Cellular communication between radiation-specific immune cells was explored by cell-to-cell communication events. The results suggest that radiotherapy trigger changes in immune cell subsets, which then reprogram the immune ecosystem and mediate systemic bystander effects. These radiation-specific immune cells participate in a wide range of cell-to-cell communication events. In particular, radiation-specific CD8+T cells appear to be at the core of communication and appear to persist in the body after recovery from radiotherapy, with enrichment analysis showing that radiation-specific CD8+ T cells are associated with ferroptosis. Thus, radiation-specific CD8+ T cells may be involved in cellular ferroptosis-mediated adverse effects caused by RT.

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“…Besides, their concentrations in the creeping wood sorrel were low (see below). Second, lauric acid is known to be contained in mulberry leaves [93,94]. However, because lauric acid is volatile, it may be minimized during an autoclave sterilization process of Silk Mate L4M.…”

Section: Artificial Diet Preparationmentioning

confidence: 99%

Radioactive Pollution and Biological Effects of Radioactivity

2023

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Blood Gene Expression Profile Study Revealed the Activation of Apoptosis and p53 Signaling Pathway May Be the Potential Molecular Mechanisms of Ionizing Radiation Damage and Radiation-Induced Bystander Effects

Cited by 9 publications

References 49 publications

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

Single-cell mechanistic studies of radiation-mediated bystander effects

Radioactive Pollution and Biological Effects of Radioactivity

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