Blood Grouping Techniques

Klein, Harvey G.; Anstee, David J.

doi:10.1002/9780470986868.ch8

Mollison's Blood Transfusion in Clinical Medicine 2005

DOI: 10.1002/9780470986868.ch8

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Blood Grouping Techniques

Harvey G. Klein¹,

David J. Anstee²

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Cited by 2 publications

References 253 publications

(166 reference statements)

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A new reliable test for crossmatching: microplate hydrogel immunoassay technology

Chen

Wang

Ding

et al. 2019

Transfusion Medicine

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SUMMARY Objectives To provide a novel assay to detect incomplete antibodies in the crossmatching test. Background There is a requirement in China that both major and minor crossmatch tests are required. Among all methods of crossmatching, the tube anti‐human globulin test requires tedious washing steps and is time‐consuming, whereas the microcolumn gel immunoassay anti‐human globulin test is susceptible to sample quality. Methods The process of the microplate hydrogel immunoassay anti‐human globulin test involves the use of our patented hydrogel chromatography medium and U‐bottom microplates pre‐coated with goat anti‐human globulin (AHG). A mixture of red blood cells (RBCs) and serum is centrifuged through the hydrogel under precise conditions. In incompatible reactions, the sensitised RBCs are captured by the pre‐coated AHG and form a layer over the bottom of the well, whereas in compatible reactions, the unbound RBCs form a button at the bottom of the well. The sensitivity of this new approach and the performance when testing old specimens were evaluated. Results This approach was more convenient and slightly more sensitive than the tube anti‐human globulin test and was superior to the microcolumn gel immunoassay when testing old specimens. Conclusion In general, this assay is suitable for the routine clinical use of crossmatching to detect incomplete antibodies.

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A new reliable test for crossmatching: microplate hydrogel immunoassay technology

Chen

Wang

Ding

et al. 2019

Transfusion Medicine

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Diagnostic Usefulness of Antibody Identification Test Including Enzyme Method

Lee

Shin

et al. 2006

Korean J Hematol

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Background: Many medical institutions in Korea have recently been performing an antibody screening test as one of the essential elements of a pre-transfusion test. In this study we will determine the advantage and clinical significance of adding an enzyme method to the antiglobulin method while conducting the antibody identification test. Methods: We performed antibody identification tests between December 2002 and December 2005, for a total of 37 months at Pusan National University Hospital. In this study we have analyzed 550 cases that were conducted by both the antiglobulin method and the enzyme method at the same time. Results: A total of 111 of the results were cases of detection using the adding an enzyme method. Among these results, Rh antibodies that included the anti-E had the highest number of results 77 (69.4%), 28 antibody (25.2%), 2 anti-P1 (1.8%) and one anti-Jk b (0.9%).

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Blood Grouping Techniques

Cited by 2 publications

References 253 publications

A new reliable test for crossmatching: microplate hydrogel immunoassay technology

A new reliable test for crossmatching: microplate hydrogel immunoassay technology

Diagnostic Usefulness of Antibody Identification Test Including Enzyme Method

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