Blood pressure pulsations modulate olfactory bulb neuronal activity via mechanosensitive ion channels

Jammal, Luna; Bitzenhofer, Sebastian H.; Hanganu‐Opatz, Ileana L.; Dutschmann, Mathias; Egger, Veronica

doi:10.1101/2023.07.27.550787

Cited by 3 publications

(1 citation statement)

References 107 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For mesenchymal stem cell (MSC) fate commitment, increasing viscous properties in a stiff matrix promotes greater MSC osteogenic differentiation whereas reducing viscous properties in a soft matrix promotes adipogenic differentiation. 5 Careful consideration of ECM viscous properties is particularly important in brain tissue, which is ~80% water 9 and exquisitely sensitive to external shear forces, 10 internal dynamics of blood and cerebrospinal fluid flow, 11 and changes in intracranial pressure. 12 For example, the viscoelasticity of brain tissue is increasingly understood to play important roles in the propagation of stresses in traumatic brain injury (TBI), 13 which has in turn spurred efforts to incorporate viscoelastic properties into therapeutic materials for healing brain lesions associated with TBI and other pathologies.…”

Section: Introductionmentioning

confidence: 99%

Viscoelastic high-molecular-weight hyaluronic acid hydrogels support rapid glioblastoma cell invasion with leader-follower dynamics

Carvalho,

Ding,

Saha

et al. 2024

Preprint

View full text Add to dashboard Cite

Hyaluronic acid (HA), the primary component of brain extracellular matrix, is increasingly used to model neuropathological processes, including glioblastoma (GBM) tumor invasion. While elastic hydrogels based on crosslinked low-molecular-weight (LMW) HA are widely exploited for this purpose and have proven valuable for discovery and screening, brain tissue is both viscoelastic and rich in high-MW (HMW) HA, and it remains unclear how these differences influence invasion. To address this question, hydrogels comprised of either HMW (1.5 MDa) or LMW (60 kDa) HA are introduced, characterized, and applied in GBM invasion studies. Unlike LMW HA hydrogels, HMW HA hydrogels relax stresses quickly, to a similar extent as brain tissue, and to a greater extent than many conventional HA-based scaffolds. GBM cells implanted within HMW HA hydrogels invade much more rapidly than in their LMW HA counterparts and exhibit distinct leader-follower dynamics. Leader cells adopt dendritic morphologies, similar to invasive GBM cells observed in vivo. Transcriptomic, pharmacologic, and imaging studies suggest that leader cells exploit hyaluronidase, an enzyme strongly enriched in human GBMs, to prime a path for followers. This study offers new insight into how HA viscoelastic properties drive invasion and argues for the use of highly stress-relaxing materials to model GBM.

show abstract

Section: Introductionmentioning

confidence: 99%

Viscoelastic high-molecular-weight hyaluronic acid hydrogels support rapid glioblastoma cell invasion with leader-follower dynamics

Carvalho,

Ding,

Saha

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Blood pressure pulsations modulate central neuronal activity via mechanosensitive ion channels

Jammal Salameh,

Bitzenhofer,

Hanganu-Opatz

et al. 2024

Science

Self Cite

View full text Add to dashboard Cite

The transmission of the heartbeat through the cerebral vascular system causes intracranial pressure pulsations. We discovered that arterial pressure pulsations can directly modulate central neuronal activity. In a semi-intact rat brain preparation, vascular pressure pulsations elicited correlated local field oscillations in the olfactory bulb mitral cell layer. These oscillations did not require synaptic transmission but reflected baroreceptive transduction in mitral cells. This transduction was mediated by a fast excitatory mechanosensitive ion channel and modulated neuronal spiking activity. In awake animals, the heartbeat entrained the activity of a subset of olfactory bulb neurons within ~20 milliseconds. Thus, we propose that this fast, intrinsic interoceptive mechanism can modulate perception—for example, during arousal—within the olfactory bulb and possibly across various other brain areas.

show abstract

Pressure Pulsatility Links Cardio-Respiratory and Brain Rhythmicity

Hamill

2023

J. Integr. Neurosci.

View full text Add to dashboard Cite

This article presents evidence indicating that intracranial pressure (ICP) pulsatility, associated with the heartbeat and breathing, is not just a source of mechanical artefact in electrical recordings, but is “sensed” and plays a role in the brain’s information processing. Patch-clamp recording of pressure-activated channels, and detection of Piezo2-protein channel expression in brain neurons, suggest that these channels provide neurons with an intrinsic resonance to ICP pulsatility, which acts to synchronize remote neural networks. Direct measurements in human patients indicate that heartbeat and breathing rhythms generate intracranial forces of tens of millinewtons, exceeding by orders of magnitude the localized forces shown by atomic force microscopy and optical tweezers to activate Piezo channels in isolated neocortical and hippocampal neurons. Additionally, many human touch and proprioceptors, which are also transduced by Piezo channels, show spiking that is phase-locked to heartbeat- and breathing-induced extracranial pressure pulsations. Finally, based on the observation that low-frequency oscillations modulate the phase and amplitude of high-frequency oscillations, body and brain oscillations are proposed to form a single hierarchical system in which the heartbeat is the basic frequency and scaling factor for all other oscillations. Together, these results support the idea that ICP pulsatility may be elemental in modulating the brain’s electrical rhythmicity.

show abstract

Blood pressure pulsations modulate olfactory bulb neuronal activity via mechanosensitive ion channels

Cited by 3 publications

References 107 publications

Viscoelastic high-molecular-weight hyaluronic acid hydrogels support rapid glioblastoma cell invasion with leader-follower dynamics

Viscoelastic high-molecular-weight hyaluronic acid hydrogels support rapid glioblastoma cell invasion with leader-follower dynamics

Blood pressure pulsations modulate central neuronal activity via mechanosensitive ion channels

Pressure Pulsatility Links Cardio-Respiratory and Brain Rhythmicity

Contact Info

Product

Resources

About