Blood small extracellular vesicles derived miRNAs to differentiate pancreatic ductal adenocarcinoma from chronic pancreatitis

Guo, Shiwei; Qin, Hao; Liu, Ke; Wang, Huan; Bai, Songling; Liu, Shiyi; Shao, Zhuo; Zhang, Yanan; Song, Bin; Xu, Xiang; Shen, Jing; Zeng, Peng; Shi, Xiaohan; Chen, Hao; Gao, Suizhi; Xu, Jiajia; Pan, Yaqi; Xiong, Lei; Li, Fugen; Zhang, Dadong; Jiao, Xiao‐Dong; Jin, Gang

doi:10.1002/ctm2.520

Cited by 13 publications

(15 citation statements)

References 55 publications

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“…The sensitivity and specificity of these miRNAs were higher than 80%. Several studies have been reported the deregulation of miR-142-3p ( Lv et al, 2012 ; Hu et al, 2013 ; Wang et al, 2018 ; Gao et al, 2019 ; Liu et al, 2021 ), miR-199a-5p ( Zhou et al, 2015 ; Zhang et al, 2021 ), miR-223-5p ( Gilicze et al, 2014 ; Zhu et al, 2019 ; Deng et al, 2021 ), let-7d-5p ( De Santis and Götte, 2021 ; Li et al, 2021 ), miR-148b-3p ( Mollazadeh et al, 2019 ; Yuan et al, 2019 ; Shan et al, 2021 ), miR-340-5p ( Guo et al, 2021 ; Huang et al, 2021 ; Tan et al, 2021 ), and miR-421 ( Chen et al, 2019 ; Mo et al, 2020 ) in the blood of different cancer types. The KEGG pathway analysis showed that the microRNAs in cancer and P53 signaling pathway were the most enriched pathways.…”

Section: Discussionmentioning

confidence: 99%

A panel of blood-derived miRNAs with a stable expression pattern as a potential pan-cancer detection signature

Sabbaghian

Mussack

Kirchner

et al. 2022

Front. Mol. Biosci.

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Introduction: MicroRNAs have a significant role in the regulation of the transcriptome. Several miRNAs have been proposed as potential biomarkers in different malignancies. However, contradictory results have been reported on the capability of miRNA biomarkers in cancer detection. The human biological clock involves molecular mechanisms that regulate several genes over time. Therefore, the sampling time becomes one of the significant factors in gene expression studies.Method: In the present study, we have tried to find miRNAs with minimum fluctuation in expression levels at different time points that could be more accurate candidates as diagnostic biomarkers. The small RNA-seq raw data of ten healthy individuals across nine-time points were analyzed to identify miRNAs with stable expression.Results: We have found five oscillation patterns. The stable miRNAs were investigated in 779 small-RNA-seq datasets of eleven cancer types. All miRNAs with the highest differential expression were selected for further analysis. The selected miRNAs were explored for functional pathways. The predominantly enriched pathways were miRNA in cancer and the P53-signaling pathway. Finally, we have found seven miRNAs, including miR-142-3p, miR-199a-5p, miR-223-5p, let-7d-5p, miR-148b-3p, miR-340-5p, and miR-421. These miRNAs showed minimum fluctuation in healthy blood and were dysregulated in the blood of eleven cancer types. Conclusion: We have found a signature of seven stable miRNAs which dysregulate in several cancer types and may serve as potential pan-cancer biomarkers.

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Section: Discussionmentioning

confidence: 99%

A panel of blood-derived miRNAs with a stable expression pattern as a potential pan-cancer detection signature

Sabbaghian

Mussack

Kirchner

et al. 2022

Front. Mol. Biosci.

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“…Bayesian network analysis demonstrated that miR-95-3p was associated with PDAC, and miR-26b-5p was associated with pancreatitis. miR-95-3p/miR-26b-5p and its combination with CA-19-9 could separate PDAC from CP, and miR-335-5p/miR-340-5p was identified to associate with PDAC metastasis and poor prognosis [ 175 ].…”

Section: Biomarkers and Therapeutic Approachesmentioning

confidence: 99%

The Role of MicroRNAs in Pancreatitis Development and Progression

Patel

Almanzar

LaComb

et al. 2023

IJMS

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Pancreatitis (acute and chronic) is an inflammatory disease associated with significant morbidity, including a high rate of hospitalization and mortality. MicroRNAs (miRs) are essential post-transcriptional modulators of gene expression. They are crucial in many diseases’ development and progression. Recent studies have demonstrated aberrant miRs expression patterns in pancreatic tissues obtained from patients experiencing acute and chronic pancreatitis compared to tissues from unaffected individuals. Increasing evidence showed that miRs regulate multiple aspects of pancreatic acinar biology, such as autophagy, mitophagy, and migration, impact local and systemic inflammation and, thus, are involved in the disease development and progression. Notably, multiple miRs act on pancreatic acinar cells and regulate the transduction of signals between pancreatic acinar cells, pancreatic stellate cells, and immune cells, and provide a complex interaction network between these cells. Importantly, recent studies from various animal models and patients’ data combined with advanced detection techniques support their importance in diagnosing and treating pancreatitis. In this review, we plan to provide an up-to-date summary of the role of miRs in the development and progression of pancreatitis.

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“…On the other hand, LB techniques, which involve collecting tissue, blood, or urine, offer a potentially more cost-effective alternative. Traditional protein markers such as CA19-9 and CEA can be detected using commercial products such as IMMray ® PanCan-d test, Galleri ® test, and CellSearch ® test, with costs ranging from USD 900 to USD 995 [ 216 , 217 , 218 ] in Table 1 .…”

Section: Market Of Pc Screening Testmentioning

confidence: 99%

Pancreatic Cancer and Detection Methods

Ungkulpasvich,

Hatakeyama,

Hirotsu

et al. 2023

Biomedicines

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The pancreas is a vital organ with exocrine and endocrine functions. Pancreatitis is an inflammation of the pancreas caused by alcohol consumption and gallstones. This condition can heighten the risk of pancreatic cancer (PC), a challenging disease with a high mortality rate. Genetic and epigenetic factors contribute significantly to PC development, along with other risk factors. Early detection is crucial for improving PC outcomes. Diagnostic methods, including imagining modalities and tissue biopsy, aid in the detection and analysis of PC. In contrast, liquid biopsy (LB) shows promise in early tumor detection by assessing biomarkers in bodily fluids. Understanding the function of the pancreas, associated diseases, risk factors, and available diagnostic methods is essential for effective management and early PC detection. The current clinical examination of PC is challenging due to its asymptomatic early stages and limitations of highly precise diagnostics. Screening is recommended for high-risk populations and individuals with potential benign tumors. Among various PC screening methods, the N-NOSE plus pancreas test stands out with its high AUC of 0.865. Compared to other commercial products, the N-NOSE plus pancreas test offers a cost-effective solution for early detection. However, additional diagnostic tests are required for confirmation. Further research, validation, and the development of non-invasive screening methods and standardized scoring systems are crucial to enhance PC detection and improve patient outcomes. This review outlines the context of pancreatic cancer and the challenges for early detection.

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Blood small extracellular vesicles derived miRNAs to differentiate pancreatic ductal adenocarcinoma from chronic pancreatitis

Cited by 13 publications

References 55 publications

A panel of blood-derived miRNAs with a stable expression pattern as a potential pan-cancer detection signature

A panel of blood-derived miRNAs with a stable expression pattern as a potential pan-cancer detection signature

The Role of MicroRNAs in Pancreatitis Development and Progression

Pancreatic Cancer and Detection Methods

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