Blood-Tissue Barriers

França, Luiz R.; Auharek, Sarah Alves; Hess, Rex A.; Dufour, Jannette M.; Hinton, Barry T.

doi:10.1007/978-1-4614-4711-5_12

Cited by 106 publications

(9 citation statements)

References 154 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[3][4][5][6] In the epididymis, for instance, TJs between epithelial cells are responsible for the formation of the bloodepididymal barrier (BEB) and represent a key element of epididymal physiology. 7,8,9 The BEB consists of a physical, physiological and immunological barrier that regulates the luminal milieu of the epididymal duct, protecting auto-antigenic sperm from the immune system. Moreover, it contributes to the composition of a unique luminal environment required for sperm maturation, besides maintaining apical/basolateral epithelial cell polarity.…”

Section: Introductionmentioning

confidence: 99%

Distribution pattern of ZO-1 and claudins in the epididymis of vampire bats

et al. 2020

View full text Add to dashboard Cite

Epithelial cells connect with each other by tight junctions (TJs) in several tissues. In epididymides, TJs proteins form the blood-epididymis barrier (BEB), which is crucial for male fertility. However, little is known about BEB morphological and physiological aspects in wild animals. This study examines the region-specific distribution pattern of TJs proteins in D. rotundus' epididymis, assessing their regulation in rainy and dry season. The expression of zonula occludens-1 (ZO-1), and claudins (Cldn)-1, −3, and −4 were evaluated by confocal immunofluorescence and ELISA analysis. Herein, ZO-1 was strictly expressed in TJs, whereas Cldns were expressed in TJs and basolateral membranes of epithelial cells. Their co-localization and intensity of expression varied in the epididymal regions examined. The effect of season on protein expression was detected mainly in TJ proteins located in the proximal regions. As such, in the initial segment (IS), Cldn-3 and −4 were detected at low levels in basolateral membranes in the rainy season compared to the dry season. Furthermore, in the distal IS, Cldn-1 expression was lower in TJs of epithelial cells during the rainy season than the dry season. ZO-1 expression was higher in the cauda region than the corpus region by ELISA analysis. Additionally, in the corpus region, ZO-1 expression was higher in TJs during dry season compared to the rainy season. Our study sheds light on the understanding of BEB in D. rotundus, improving the knowledge of their reproductive biology.

show abstract

Section: Introductionmentioning

confidence: 99%

Distribution pattern of ZO-1 and claudins in the epididymis of vampire bats

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Also, it is an exciting model system for studying how F-actin dynamics facilitates coordinated restructuring of cellular junctions around developing germ cells and transports them across an epithelium. In vertebrates, the germline cells divide and differentiate within an enclosure of somatic Sertoli cells that form the seminiferous tubules (Franç a et al, 2012;Jé gou, 1993). The differentiating spermatids also advance toward the lumen of the tubule by negotiating a distinctive set of dynamic junctions found between the Sertoli cells (Russell, 1977;Wang and Cheng, 2007;Xiao et al, 2014a).…”

Section: Introductionmentioning

confidence: 99%

Localized, Reactive F-Actin Dynamics Prevents Abnormal Somatic Cell Penetration by Mature Spermatids

2016

View full text Add to dashboard Cite

Spermatogenesis occurs inside a somatic cell enclosure. Sperm release, the most important final step and a target for contraceptives, has been extensively studied in fixed tissue preparations. Here, we provide a time-lapse description of the release process in Drosophila testis ex vivo. We show that the spermatid tails exit the somatic enclosure and enter the testicular duct first, followed by the spermatid heads. Prior to this, individual spermatid heads attempt to invade the head cyst cell, and on each occasion they are repelled by a rapid and local F-actin polymerization response from the head cyst cell. The F-actin assembly involves N-WASp, D-WIP, and Arp2/3 complex and dissipates once the spermatid head retreats back into the fold. These findings revise the existing spermiation model in Drosophila and suggest that somatic cells can actively oppose mechanical cell invasion attempts using calibrated F-actin dynamics in situ.

show abstract

“…Therefore, this study confirmed for the first time that CNP has anti-inflammatory and immunomodulatory effects on acute epididymitis, and its mechanism was revealed. Currently, epididymal immunological regulation mainly depends on the following four aspects: ① Blood-epididymal barrier (Franca et al, 2012): Sperm can avoid immune attack through the barrier. Infection can change or destroy the barrier, causing immune response and sperm function damage (Fijak et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

C-Type Natriuretic Peptide Plays an Anti-Inflammatory Role in Rat Epididymitis Induced by UPEC

Mei

Zhang

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Human epididymitis is mainly caused by retrograde urinary tract infection with uropathogenic Escherichia coli (UPEC). This disease is an important factor (accounting for 20–30%) causing male infertility. C-type natriuretic peptide (CNP), a protein composed of 22 amino acids, is proved to play an immunoregulatory role in respiratory and cardiovascular systems. CNP is expressed extremely high in the epididymis, but whether CNP plays the same role in acute epididymitis is unclear. At first, we established an acute caput epididymitis model in rats with UPEC and treated them with CNP to measure inflammatory damage. Then RNA-seq transcriptome technology was used to reveal potential signal pathways. Secondly, the turbidity and activity of UPEC were assessed using a microplate reader and the amount of UPEC by agar plates after incubation with CNP. Thirdly, macrophages in caput epididymis were tested by immunohistochemistry (IHC). Meanwhile, lipopolysaccharide (LPS) with or without CNP was used to stimulate the macrophage (RAW264.7) in vitro and to detect the expression level of pro-inflammatory factors. Finally, the macrophage (RAW264.7) was treated with CNP, 8-Br-cGMP [cyclic guanosinc monophosphate (cGMP) analog] and KT5823 [protein kinase G (PKG) inhibitor], and the expression level of nuclear factor-k-gene binding (NF-kB) signal pathway was examined. The results showed that the damage of epididymis induced by UPEC as well as the pro-inflammatory factors could be alleviated significantly with CNP treatment. CNP could inhibit the activity and numbers of bacteria in both in vivo and in vitro experiments. Moreover, CNP repressed the invasion, and the expression of pro-inflammatory factors (such as NF-kB, IL-1β, IL-6, TNF-α) in macrophages and its effect could be inhibited by KT5823. Therefore, we drew a conclusion from the above experiments that CNP alleviates the acute epididymitis injury induced by UPEC. On one hand, CNP could inhibit the growth of UPEC. On the other hand, CNP could decrease invasion and inflammatory reaction of macrophages; the mechanism was involved in inhibiting NF-kB signal pathway through the cGMP/PKG in macrophages. This research would open up the possibility of using CNP as a potential treatment for epididymitis.

show abstract

Blood-Tissue Barriers

Cited by 106 publications

References 154 publications

Distribution pattern of ZO-1 and claudins in the epididymis of vampire bats

Distribution pattern of ZO-1 and claudins in the epididymis of vampire bats

Localized, Reactive F-Actin Dynamics Prevents Abnormal Somatic Cell Penetration by Mature Spermatids

C-Type Natriuretic Peptide Plays an Anti-Inflammatory Role in Rat Epididymitis Induced by UPEC

Contact Info

Product

Resources

About