Blood vessel invasion and expression of sialyl lewisx and proliferating cell nuclear antigen in stage I non-small cell lung cancer. Relation to postoperative recurrence

Ogawa, Jun–ichi; Tsurumi, Toyohiko; Yamada, Shunsuke; Koide, Shirosaku; Shohtsu, Akira

doi:10.1002/1097-0142(19940215)73:4<1177::aid-cncr2820730409>3.0.co;2-0

Cited by 80 publications

(49 citation statements)

References 19 publications

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“…In contrast to ploidy, SPF was not associated with prognosis in this study. This is in agreement with the observations of some investigators, but conflicts with the results of others who have shown a correlation between survival and SPF in lung cancer [6,13,24,41].…”

Section: Discussionsupporting

confidence: 89%

DNA Aneuploidy, S-phase fraction, nuclear p53 positivity, and survival in non-small-cell lung carcinoma

et al. 1997

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Inactivation of the p53 gene plays a key role in tumour biology, probably through a disturbed cell cycle control and an increased genetic instability in p53-inactivated tumours. To learn more about the relationship between p53 alterations, proliferation and genetic instability (DNA aneuploidy) in lung cancer patients, specimens of 220 surgically resected lung carcinomas with clinical follow-up information were examined by immunohistochemistry (p53; CM1) and flow cytometry. Nuclear p53 positivity--found in 49.5% of the tumours--was associated with both high S-phase fraction (SPF) and DNA ploidy aberrations. SPF was higher in p53-positive tumours (15.9 +/- 10.2) than in p53-negative tumours (10.3 +/- 8.7; P = 0.03). The rate of p53 positivity was higher in 101 DNA-aneuploid and DNA-multiploid tumours (55%) than in 27 diploid and peridiploid carcinomas (33%; P = 0.0512). These results are consistent with an in vivo role of p53 inactivation for increased proliferative activity and development of genomic instability in lung cancer. There was no association between SPF and prognosis. Although prognosis was worse in DNA-aneuploid and multiploid tumours than in diploid, peridiploid and tetraploid carcinomas (P = 0.029), DNA ploidy was not an independent predictor of poor prognosis in multivariate analysis. These data show that DNA-flow cytometry has little prognostic value for patients with resected non-small-cell lung carcinoma.

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Section: Discussionsupporting

confidence: 89%

DNA Aneuploidy, S-phase fraction, nuclear p53 positivity, and survival in non-small-cell lung carcinoma

et al. 1997

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“…The presence of carbohydrate receptor sLe X in tumors or serum has been associated well with poor prognosis of various cancers [12,14,16,17] and suggested no other carbohydrate epitope examined to date has equal prognostic value. sLe X expressions in colorectal and non-small cell lung cancer has been correlated with advanced disease, increased distant metastasis and decreased survival [41]. An in vitro study identified molecules capable of interfering metastatic process by inhibiting sLe X -mediated adhesion between tumor cells and activated endothelial cells [42].…”

Section: Discussionmentioning

confidence: 99%

E-Cadherin truncation and Sialyl Lewis-X overexpression in oral squamous cell carcinoma and oral precancerous conditions

Shah¹,

Sainger²,

Telang³

et al. 2009

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The present study aimed to determine significance of E-cadherin, a cell adhesion molecule, and sialyl Lewis-X (sLe X ), a cell surface antigen, in oral carcinogenesis. Expressions of E-cadherin and sLe X were detected using western blot analysis from oral malignant (n=25), and oral precancerous tissues (OPC, n=20) and their adjacent normal tissues. An altered expression of E-cadherin (E-cad) and sLe X was observed in malignant and precancerous tissues. E-cad western blot revealed presence of two bands, a 120 kDa (native, E-cad 120 ) and a 97 kDa (known as truncated E-cad 97 ). The accumulation of truncated E-cad 97 and sLe X in malignant and OPC tissues compared to their adjacent normal tissues was observed. Receiver's Operating Characteristics (ROC) curve analysis showed good discriminatory efficacy of E-cad 97 , E-cad 97:120 ratio and sLe X between the malignant and adjacent. normal tissues. Further, a positive correlation of E-cad 97 and sLe X overexpression with advanced stage of the disease and lymphnode metastasis was observed. The data suggest that E-cadherin truncation and sLe X overexpression are early events which may facilitate the tumor cells to metastasize. Also, overexpression E-cad 97 and sLe X in OPC tissues may be useful to predict metastatic potentials of tumors at an early stage of oral carcinogenesis.

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“…It was well-documented that Lewis antigens, mainly located on the outer chains of the glycans of cell surface glycolipids or the O-linked glycans of glycoproteins, were closely related to the metastatic potential and overall prognosis of many human cancers, such as colorectal, prostate and lung cancer (Nakamori et al, 1993;Ogawa et al, 1994;Jorgensen et al, 1995). Lewis antigens are a series of fucosylated Galβ1,3/1,4GlcNAc containing oligosaccharides (sialylated or not sialylated, β1,3 linkage in type 1 chain and β1,4 linkage in type II chain), and are known to participate in the process of leukocyte infiltration during inflammation and cell adhesion during malignant cell metastasis.…”

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confidence: 99%

Regulation of differentiation- and proliferation-inducers on Lewis antigens, α-fucosyltransferase and metastatic potential in hepatocarcinoma cells

Liu

Chen

2001

Br J Cancer

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SummaryThe expressions of Lewis (Le) antigens, α-1,3/1,4 fucosyltransferases (α-1,3/1,4 FuTs), and metastatic potential after the treatment of 2 differentiation inducers, all-trans retinoic acid (ATRA), 8-bromo-cyclic 3′,5′adenosine monophosphate (8-Br-cAMP); and 2 proliferation inducers, epidermal growth factor (EGF) and phobol-12-myristate-13-acetate (PMA), on 7721 human hepatocarcinoma cell line were studied. Cell adhesion to human umbilical vein endothelial cells (HUVEC), cell migration through transwell and invasion through matrigel were selected as the indexes of metastatic potential-related phenotypes. Using fluorescence-labelled antibodies and flow-cytometric analysis, it was found that 7721 cells mainly expressed sialyl Lewis X (SLe x ) and a less amount of sialyl dimeric Lewis X (SDLe x ) antigens on the cell surface. Their expressions were down-regulated by ATRA, and up-regulated by EGF. SLe x antigen was also decreased and increased by the treatment of 8-Br-cAMP and PMA respectively. With Northern blot to detect the mRNAs of α-1,3/1,4 FuTs, the main enzymatic basis for the change in SLe x expression was found to be the alteration of the expression of α-1,3 FuT-VII. It was evidenced by the observations that α-1,3 FuT-VII was the main α-1,3/1,4 FuT in 7721 cells, while α-1,3/1,4 FuT-III and α-1,3 FuT-VI were expressed rather low. The changes in the expressions of SLe x antigen and α-1,3 FuT-VII resulted in the altered cell adhesion to tumour necrosis factor-α stimulated HUVEC, since only the monoclonal antibody of the SLe

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Blood vessel invasion and expression of sialyl lewisx and proliferating cell nuclear antigen in stage I non-small cell lung cancer. Relation to postoperative recurrence

Cited by 80 publications

References 19 publications

DNA Aneuploidy, S-phase fraction, nuclear p53 positivity, and survival in non-small-cell lung carcinoma

DNA Aneuploidy, S-phase fraction, nuclear p53 positivity, and survival in non-small-cell lung carcinoma

E-Cadherin truncation and Sialyl Lewis-X overexpression in oral squamous cell carcinoma and oral precancerous conditions

Regulation of differentiation- and proliferation-inducers on Lewis antigens, α-fucosyltransferase and metastatic potential in hepatocarcinoma cells

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