Blood vessels are primary targets for 2,3,7,8-tetrachlorodibenzo-p-dioxin in pre-cardiac edema formation in larval zebrafish

Nijoukubo, Daisuke; Adachi, Hikaru; Kitazawa, Takio

doi:10.1016/j.chemosphere.2020.126808

Cited by 6 publications

(3 citation statements)

References 48 publications

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“…Edema generation is regarded as a common feature of developmental toxicity. Reduced blood flow and irregular cardiac output related to problematic blood vessels because of certain contaminants caused pericardial edema and circulation failure; moreover, the inhibitory effect in the enzymatic pathway induced edema (Teraoka et al, 2014;Nijoukubo et al, 2020). During vertebrate development, functional heart differentiates with the help of cardiac progenitors into the myocardium, ventricles, and endocardium to establish cardiac contractility for nutrient delivery, oxygen supply, and nerves/lymphatic system stability, whereas structural loss in the conduction system precedes development abnormalities, ultimately causing lethality (Staudt and Stainier, 2012).…”

Section: Discussionmentioning

confidence: 99%

Isoprocarb induces acute toxicity in developing zebrafish embryos through vascular malformation

Parkhahyun

Song

Lim

2021

J Anim Reprod Biotechnol

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In this study, the potential toxicity of isoprocarb was demonstrated using zebrafish embryos. We treated isoprocarb (0, 29, and 58 mg/L) to the zebrafish embryos for 72 h then, we estimated morphological changes and apoptotic cell numbers. The increasing extent of apoptosis from the anterior to posterior region of developing zebrafish larvae was correlated with toxicity in the overall development process, including growth and normal organ formation. The appearance of abnormalities in the isoprocarb-treated groups in comparison to normal developing zebrafish larvae was verified using quantitative image analysis based on ImageJ software program. The vascular system comprising a complex interconnection of blood vessels was visualized in vessel-fluorescent transgenic zebrafish (fli1:eGFP). The main vasculature was malformed on isoprocarb treatment, and this was also related to cardiac defects. Taken together, normal embryonic development in zebrafish was interrupted owing to the acute toxicity of isoprocarb.

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Section: Discussionmentioning

confidence: 99%

Isoprocarb induces acute toxicity in developing zebrafish embryos through vascular malformation

Parkhahyun

Song

Lim

2021

J Anim Reprod Biotechnol

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“…Adult zebrafish were maintained at 28.5°C in a re-circulating aquatic habitat system under a 14 h:10 h (light:dark) photoperiod as previously reported [ 55 ]. Female and male adult zebrafish were mated at a ratio of 1:1, and the embryos were collected and maintained in Zebrafish Ringer solution (ZR solution) (38.7 mM NaCl, 1.0 mM KCl, 1.7 mM HEPES-NaOH pH 7.2, 2.4 mM CaCl 2 ) at 28.5°C in an incubator.…”

Section: Methodsmentioning

confidence: 99%

Augmentation of Pectoral Fin Teratogenicity by Thalidomide in Human Cytochrome P450 3A-Expressing Zebrafish

et al. 2023

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The pharmacological and toxicological effects of active metabolites of enzymes including cytochrome P450 (CYP) are important. While it has been believed for a long time that thalidomide causes characteristic limb malformation only in rabbits and primates including humans, the involvement of their CYP3A subtypes (CYP3As) has been suggested. Recently, however, it was reported that zebrafish were sensitive to thalidomide, showing defects of pectoral fins, homologous organs of forelimbs in mammals, as well as other deformities. In this study, we prepared human CYP3A7 (hCYP3A7)-expressing zebrafish (F0) using a transposon system. Thalidomide caused pectoral fin defects and other malformations including pericardial edema in hCYP3A7-expressing embryos/larvae but not in wild-type and hCYP1A1-expressing embryos/larvae. Thalidomide also reduced the expression of fibroblast growth factor 8 in pectoral fin buds in only hCYP3A7-expressing embryos/larvae. The results suggest the involvement of human-type CYP3A in thalidomide teratogenicity.

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“…Their distribution in the environment is mainly due to their persistency and bioaccumulation in the food chain. Several studies have reported that 2,3,7,8-Tetrachlorodibenzo-P-dioxin (2,3,7,8-TCDD), which is the most toxic of all PCBs, causes chloracne in humans, skeletal deformities, kidney defects, and weakened immune responses [ 6 ]. Figure 1 showed the structure of 2,3,7,8-TCDD.…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of a 2,3,7,8-Tetrachlorodibenzo-P-dioxin degrading strain and its biochemical degradation pathway

Qiu

Zhang

Gong

et al. 2021

J Environ Health Sci Engineer

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This study aims to find a high-efficiency degradation strain which can biodegrade the 2,3,7,8-Tetrachlorodibenzo-P-dioxin (2,3,7,8-TCDD). In this paper, a new fungus strain was isolated from activated sludge of Dagu Drainage River in Tianjin which was able to degrade 2,3,7,8-TCDD in the medium. Based on its morphology and phylogenetic analysis of its 18S rDNA sequence, the strain was identified as Penicillium sp. QI-1. Response surface methodology using central composite rotatable design of cultural conditions was successfully employed for optimization resulting in 87.9 % degradation of 2,3,7,8-TCDD (1 µg/mL) within 6 days. The optimum condition for degrading 2,3,7,8-TCDD was at 31℃ and pH 7.4. The biodegradation process was fitted to a first-order kinetic model. The kinetic equation was Ct=0.939e− 0.133t and its half-life was 5.21d. The fungus strain degraded 2,3,7,8-TCDD to form intermediates, they were 4,5-Dichloro-1,2-benzoquinone, 4,5-Dichlorocatechol, 2-Hydrooxy-1,4-benzoquinone, 1,2,4-Trihydroxybenzene and β-ketoadipic acid. A novel degradation pathway for 2,3,7,8-TCDD was proposed based on analysis of these metabolites. The results suggest that Penicillium sp. QI-1 may be an ideal microorganism for biodegradation of the 2,3,7,8-TCDD-contaminated environments.

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Blood vessels are primary targets for 2,3,7,8-tetrachlorodibenzo-p-dioxin in pre-cardiac edema formation in larval zebrafish

Cited by 6 publications

References 48 publications

Isoprocarb induces acute toxicity in developing zebrafish embryos through vascular malformation

Isoprocarb induces acute toxicity in developing zebrafish embryos through vascular malformation

Augmentation of Pectoral Fin Teratogenicity by Thalidomide in Human Cytochrome P450 3A-Expressing Zebrafish

Isolation and identification of a 2,3,7,8-Tetrachlorodibenzo-P-dioxin degrading strain and its biochemical degradation pathway

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