Bloodgroup simulating activity in aerobic gram-negative oral bacteria cultured from fresh corpses

Hooft, P.; Voorde, Herman Van de; Dijck, Patrick Van

doi:10.1016/0379-0738(91)90157-e

Cited by 7 publications

(2 citation statements)

References 12 publications

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“…It may be an important source of blood typing error and occasional mistyping of blood groups from oral material (e.g., teeth and alveolar bone) may be caused by the aerobic gram-negative oral flora. [25]…”

Section: Discussionmentioning

confidence: 99%

ABO blood grouping from hard and soft tissues of teeth by modified absorption-elution technique

Ramnarayan¹,

Manjunath²,

Joshi³

2013

J Forensic Dent Sci

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Background:Teeth have always been known as stable tissue that can be preserved both physically and chemically for long periods of time. Blood group substances have been known to be present in both the hard and soft tissues of the teeth.Objectives:This study aimed at detection of ABO blood group substances from soft and hard tissues of teeth and also to evaluate the reliability of teeth stored for a relatively long period as a source of blood group substances by absorption–elution technique with some modifications.Results:Blood group obtained from the teeth was compared with those obtained from the blood sample. Pulp showed a very large correlation in both fresh and long-standing teeth though it decreased slightly in the latter. Hard tissue showed a large correlation in both the groups indicating that hard tissue is quite reliable to detect blood group and that there is no much difference in the reliability in both the groups. However, combining pulp and hard tissue, correlation is moderate. Correlation of blood grouping with the age, sex, and jaw distribution was carried out.Conclusion:Blood group identification from hard and soft tissues of teeth aids in the identification of an individual.

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Section: Discussionmentioning

confidence: 99%

ABO blood grouping from hard and soft tissues of teeth by modified absorption-elution technique

Ramnarayan¹,

Manjunath²,

Joshi³

2013

J Forensic Dent Sci

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“…The principle of the AE test involves the absorption of blood group-specific agglutinin to the surface of a substance having blood group agglutinogens, followed by the elution of the absorbed antibody under a high temperature, and the agglutination of the blood cells against the corresponding antigens. Cold agglutination increases the agglutination intensity [7][8][9][10][11][12][13][14][15][16][17][18]. In this study, our objective was to determine the ABO blood group from both the hard and soft tissue of the tooth by using the AE technique and also to ascertain if there are any variations in the identification of the blood groups from the teeth based on age and gender.…”

Section: Introductionmentioning

confidence: 99%

Detection of ABO Blood Groups From Dentin and Pulp by Using the Absorption-Elution Technique: A Forensic Cross-Sectional Study Among the Population of the Al Jouf Province, Saudi Arabia

Rao,

Alrayes,

Faisal Alshammari

et al. 2024

Cureus

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Background and objective Human teeth have a significant forensic importance. As they are the hardest of all human tissues, they are not just chemically stable but also their characteristics are maintained for a long time after death even in the most harsh environmental conditions. Despite the advances made in DNA analysis, fingerprinting, etc., ABO blood grouping still plays a significant role in the forensic practice in the field of personal identification, paternity disputes, and several other scenarios including the identification of mass disaster victims . The term blood groups refers to inherited antigens on the surface of red blood cells (RBCs) detected by specific antibodies. Since tooth pulp contains numerous blood vessels, blood group antigens are most certainly bound to be present in tooth pulp. Various studies have shown that blood group antigens in the pulp and dentin are preserved as long as up to two years after the demise of an individual. Absorption-elution technique has been proven to be the most sensitive, reliable, and consistent method to determine the ABO blood group from both the pulp and dentine. This study aimed to ascertain the ABO blood group from both the hard (dentin) as well as the soft tissue (pulp) of the tooth by using the absorption-elution (AE) technique and also to determine if there are any variations in identifying the blood groups from the teeth based on age and gender. Material and methods After obtaining due consent, we included patients of both genders aged between 16-60 years visiting the outpatient department (OPD) clinics at the College of Dentistry for periodontal or orthodontic extractions. One patient's blood type was determined by using the slide agglutination technique before any capillary blood extraction was performed; this patient served as a control. For this investigation, we used the pulp and powdered dentin samples taken from the dental extractions to test for the presence of ABO and Rhesus (Rh) factor antigens by using the AE method. The study samples were compared with the control for blood group determination. Statistical analysis was carried out using the chi-square test with Monte Carlo (MC) simulation to check for any correlation of blood grouping with age and gender. Results The dentin and pulp were shown to have positive blood group antigens for the ABO and Rh factors. While neither pulp nor dentin performed significantly differently in identifying the blood group antigens, pulp showed marginally higher accuracy. There was no discernible difference regarding gender or age in the dentin or pulp of any of the 45 samples studied. Conclusions For determining an individual's blood type and Rh factor, both the hard (dentin) and soft (pulp) tissues of a tooth are valid sources. This is particularly helpful in forensic medicine cases where teeth are the only remains that can be viably used to find out a person's identity.

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Blood stains of the Turin Shroud 2015: beyond personal hopes and limitations of techniques

et al. 2016

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In the early '80s, evidence was provided that, rather than a dye (red okra), hemoglobin was indeed responsible for the alleged blood stains of the Turin Shroud. Such stains were shown to belong to an MNS positive individual of the AB group, and the halos surrounding the blood stains were compatible with serum containing trace amounts of bilirubin, albumin and immunoglobulins. However, being only based on indirect and circumstantial evidence, most of these data were challenged. In the late '90s, together with the evidence of the gene coding β-globin, contamination between male and female DNA was documented on the Turin Shroud. Although the presence of male was more noticeable than female DNA, these data were considered null and void. These days, to establish that blood indisputably belongs to an MNS positive individual of the AB group, and to exclude DNA contamination, high-specificity techniques with monoclonal antibodies and molecular studies on nuclear and mitochondrial DNA are needed. Indeed, consistent with DNA contamination on the Turin Shroud, sequences from multiple subjects of different ethnic origins have been recently detected on the human mitochondrial genome extracted from dust particles of the linen. Innovative concepts are likely to come up using modern research approaches to evaluate the issue of blood stains of the Turin Shroud. Nor can we rule out the possibility that religious implications of the new findings on the Turin Shroud might be envisaged. Conceivably enough, the ongoing debate will be fierce and passionate, especially in the media.

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Bloodgroup simulating activity in aerobic gram-negative oral bacteria cultured from fresh corpses

Cited by 7 publications

References 12 publications

ABO blood grouping from hard and soft tissues of teeth by modified absorption-elution technique

ABO blood grouping from hard and soft tissues of teeth by modified absorption-elution technique

Detection of ABO Blood Groups From Dentin and Pulp by Using the Absorption-Elution Technique: A Forensic Cross-Sectional Study Among the Population of the Al Jouf Province, Saudi Arabia

Blood stains of the Turin Shroud 2015: beyond personal hopes and limitations of techniques

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