Blue LED light induces regreening in the flavedo of Valencia orange in vitro

Ma, Gang; Zhang, Lancui; Kitaya, Yoshiaki; Seoka, Mao; Kudaka, Rin; Yoshio, Masaki; Yamawaki, Kazuki; Shimada, Takehiko; Fujii, Hiroshi; Endo, Tetsuro; Kato, Masaaki

doi:10.1016/j.foodchem.2020.127621

Cited by 21 publications

(7 citation statements)

References 30 publications

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“…To study the effects of blue, red, and UV lights on the nobiletin content and gene expression, the flavedos of Ponkan were excised and placed randomly on 10 mL of MS medium supplemented with 1% (w/v) agar and 10% (w/v) sucrose according to the method of Ma et al 25 The flavedos were irradiated with blue (470 nm, 50 μmol m −2 s −1 ) LED light, red (660 nm, 50 μmol m −2 s −1 ) LED light, and UV-B (280 nm, 0.5 mW) for 1 week. The flavedos cultured in the dark were used as the control.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The data were then extracted from images by using Subio Platform software (Subio Inc., Tokyo, Japan). The gene expression was confirmed by real-time PCR according to the method of Ma et al 25 The TaqMan probe and sets of primers are shown in the Supporting Information, Table S1. The levels of gene expression were analyzed using ABI PRISM 7300 Sequence Detection System Software (Applied Biosystems) and normalized with the result of 18S ribosomal RNA.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Characterization of a Caffeic Acid 8-O-Methyltransferase from Citrus and Its Function in Nobiletin Biosynthesis

Zhang

Seoka

et al. 2021

J. Agric. Food Chem.

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Nobiletin (3′,4′,5,6,7, is a polymethoxylated flavonoid specifically accumulated in citrus fruit with numerous beneficial effects to human health. In this study, a novel O-methyltransferase (CitOMT2) was isolated from three citrus varieties, Ponkan mandarin (Citrus reticulata Blanco), Nou 6 ("King mandarin" × "Mukaku-kishu"), and Satsuma mandarin (Citrus unshiu Marc.), and its functions were characterized in vitro. The gene expression results showed that CitOMT2 was highly expressed in the two nobiletin abundant varieties of Ponkan mandarin and Nou 6. However, the expression level of CitOMT2 was low in the flavedo of Satsuma mandarin, in which only a small amount of nobiletin was accumulated. Functional analysis suggested that CitOMT2 was a caffeic acid 8-O-methyltransferase, and it catalyzed the O-methylation of 7,8-dihydroxyflavone at 8-OH. As the methylation of flavone at 8-OH was required for nobiletin biosynthesis, the results presented in this study suggested that CitOMT2 was a key gene regulating nobiletin accumulation in citrus fruit.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Characterization of a Caffeic Acid 8-O-Methyltransferase from Citrus and Its Function in Nobiletin Biosynthesis

Zhang

Seoka

et al. 2021

J. Agric. Food Chem.

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“…In addition to the ripening process, light is also a crucial factor regulating the regreening in citrus fruit. Ma et al [81] reported that the blue LED light treatment enhanced the chlorophyll contents and induced regreening in the flavedo of Valencia orange in vitro. Under the blue light, the contents of chloroplast carotenoids, lutein, β-carotene, and all-transviolaxanthin increased, while the contents of chromoplast carotenoid 9-cis-violaxanthin decreased during the regreening process along with the up-regulation of LCYe and down-regulation of LCYb2.…”

Section: Lightmentioning

confidence: 99%

Regulation of Chlorophyll and Carotenoid Metabolism in Citrus Fruit During Maturation and Regreening

Keawmanee

Zhang

et al. 2023

RAS

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Chlorophyll and carotenoid are the main pigments that are responsible for coloration of citrus fruit. The changes in their contents are related to the development stage in citrus fruit. During fruit maturation, carotenoids are massively accumulated in the flavedo and juice sacs, while the chlorophyll contents decreased rapidly. In contrast, the increase of chlorophyll content and decrease of carotenoid contents are observed in the fruit during regreening process. In addition, plant hormones and environmental conditions play important roles in the regulation of color development and the changes of chlorophyll and carotenoid contents in citrus fruit. In this review, we summarized the changes in chlorophyll and carotenoid contents in the flavedo and juice sacs of citrus fruit during fruit maturation and regreening process. Current understanding on the molecular mechanisms that regulated the chlorophyll and carotenoid metabolisms in citrus fruit are discussed. Moreover, the effects of plant hormones and environmental conditions on chlorophyll and carotenoid accumulation are also presented in this review.

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“…Light is regarded as a key factor that greatly influences regreening. As a result of irradiating the citrus fruit Valencia orange with a blue LED light, the tissue was gradually greened, and after 4 weeks, the chlorophyll content was approximately twice as high as that of the unirradiated tissue (Ma et al, 2020).…”

Section: From Chromoplastsmentioning

confidence: 99%

Diversity of Plastid Types and Their Interconversions

Choi

2021

Front. Plant Sci.

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Plastids are pivotal subcellular organelles that have evolved to perform specialized functions in plant cells, including photosynthesis and the production and storage of metabolites. They come in a variety of forms with different characteristics, enabling them to function in a diverse array of organ/tissue/cell-specific developmental processes and with a variety of environmental signals. Here, we have comprehensively reviewed the distinctive roles of plastids and their transition statuses, according to their features. Furthermore, the most recent understanding of their regulatory mechanisms is highlighted at both transcriptional and post-translational levels, with a focus on the greening and non-greening phenotypes.

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Blue LED light induces regreening in the flavedo of Valencia orange in vitro

Cited by 21 publications

References 30 publications

Characterization of a Caffeic Acid 8-O-Methyltransferase from Citrus and Its Function in Nobiletin Biosynthesis

Characterization of a Caffeic Acid 8-O-Methyltransferase from Citrus and Its Function in Nobiletin Biosynthesis

Regulation of Chlorophyll and Carotenoid Metabolism in Citrus Fruit During Maturation and Regreening

Diversity of Plastid Types and Their Interconversions

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