Blue light emitting diode induces apoptosis in lymphoid cells by stimulating autophagy

Oh, Phil-Sun; Hwang, Hyeon-Shik; Kwon, Jungkee; Kim, Hyunsoo; Kim, Minjoo; Lim, Sooman; Sohn, Myung‐Hee

doi:10.1016/j.biocel.2015.11.004

Cited by 51 publications

(49 citation statements)

References 39 publications

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“…[17,21,26,27] These studies are consistent with what we found in BMSCs. However, the underlying mechanisms by which blue LED inhibits cell growth are still unclear.…”

Section: Discussionsupporting

confidence: 83%

“…At wavelengths ranging from 400-500 nm, blue LED light, another type of LEDs, has been shown to induce cell apoptosis and reduce cell proliferation in melanomas [16], B-cell lymphomas [17], skin tumors [18], and skin dermal fibroblasts [19] by regulating autophagy, reactive oxygen species (ROS) and mitochondrial mediated signaling pathways. Additionally, blue light induces photoreceptor apoptosis and causes age-related macular degeneration, also known as retinitis pigmentosa [20].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Blue Light Emitting Diode Irradiation On the Proliferation, Apoptosis and Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

Yuan

Yan

Gong

et al. 2017

Cell Physiol Biochem

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Background/Aims: Blue light emitting diodes (LEDs) have been proven to affect the growth of several types of cells. The effects of blue LEDs have not been tested on bone marrowderived mesenchymal stem cells (BMSCs), which are important for cell-based therapy in various medical fields. Therefore, the aim of this study was to determine the effects of blue LED on the proliferation, apoptosis and osteogenic differentiation of BMSCs. Methods: BMSCs were irradiated with a blue LED light at 470 nm for 1 min, 5 min, 10 min, 30 min and 60 min or not irradiated. Cell proliferation was measured by performing cell counting and EdU staining assays. Cell apoptosis was detected by TUNEL staining. Osteogenic differentiation was evaluated by ALP and ARS staining. DCFH-DA staining and γ-H2A.X immunostaining were used to measure intracellular levels of ROS production and DNA damage. Results: Both cell counting and EdU staining assays showed that cell proliferation of BMSCs was significantly reduced upon blue LED irradiation. Furthermore, treatment of BMSCs with LED irradiation was followed by a remarkable increase in apoptosis, indicating that blue LED light induced toxic effects on BMSCs. Likewise, BMSC osteogenic differentiation was inhibited after exposure to blue LED irradiation. Further, blue LED irradiation was followed by the accumulation of ROS

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“…[17,21,26,27] These studies are consistent with what we found in BMSCs. However, the underlying mechanisms by which blue LED inhibits cell growth are still unclear.…”

Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Effects of Blue Light Emitting Diode Irradiation On the Proliferation, Apoptosis and Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

Yuan

Yan

Gong

et al. 2017

Cell Physiol Biochem

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“…We previously demonstrated that blue LED irradiation induced apoptotic cell death and inhibited early stage growth of circulating tumor cells via critical signaling pathways (Oh et al, , ). In the present study, we explored dynamic changes in cancer cells induced by blue LED irradiation.…”

Section: Discussionmentioning

confidence: 99%

“…Protein extracts were prepared as previously described (Oh et al, ). Briefly, the cells were solubilized in RIPA lysis buffer containing a protease and phosphatase inhibitor cocktail solution.…”

Section: Methodsmentioning

confidence: 99%

“…We previously reported that 450 nm blue LED irradiation induced apoptosis via a mitochondrial‐mediated signaling pathway and reduced early stage tumor growth of melanoma cells (Oh et al, ). More recently, we reported that blue LED irradiation increased the survival rate of mice with leukemia and induced B cell lymphoma apoptosis and autophagy via a mitochondrial‐mediated pathway (Oh et al, ). We found that blue LED irradiation increased intracellular superoxide anion levels and DNA damage, and subsequently led to mitochondrial dysfunction as well as autophagosome formation in circulating tumor cells.…”

Section: Introductionmentioning

confidence: 97%

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Inhibitory effect of blue light emitting diode on migration and invasion of cancer cells

Kim

et al. 2017

Journal Cellular Physiology

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The aim of this study was to determine the effects and molecular mechanism of blue light emitting diode (LED) in tumor cells. A migration and invasion assay for the metastatic behavior of mouse colon cancer CT-26 and human fibrosarcoma HT-1080 cells was performed. Cancer cell migration-related proteins were identified by obtaining a 2-dimensional gel electrophoresis (2-DE) in total cellular protein profile of blue LED-irradiated cancer cells, followed by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis of proteins. Protein levels were examined by immunoblotting. Irradiation with blue LED inhibited CT-26 and HT-1080 cell migration and invasion. The anti-metastatic effects of blue LED irradiation were associated with inhibition of matrix metalloproteinase (MMP)-2 and MMP-9 expression. P38 MAPK phosphorylation was increased in blue LED-irradiated CT-26 and HT-1080 cells, but was inhibited after pretreatment with SB203580, a specific inhibitor of p38 MAPK. Inhibition of p38 MAPK phosphorylation by SB203580 treatment increased number of migratory cancer cells in CT-26 and HT-1080 cells, indicating that blue LED irradiation inhibited cancer cell migration via phosphorylation of p38 MAPK. Additionally blue LED irradiation of mice injected with CT-26 cells expressing luciferase decreased early stage lung metastasis compared to untreated control mice. These results indicate that blue LED irradiation inhibits cancer cell migration and invasion in vitro and in vivo.

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Blue light emitting diode induces apoptosis in lymphoid cells by stimulating autophagy

Cited by 51 publications

References 39 publications

Effects of Blue Light Emitting Diode Irradiation On the Proliferation, Apoptosis and Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

Effects of Blue Light Emitting Diode Irradiation On the Proliferation, Apoptosis and Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

Inhibitory effect of blue light emitting diode on migration and invasion of cancer cells

LEDsBeyond Visible Light

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