Blue light negatively affects the survival of <scp>ARPE</scp>19 cells through an action on their mitochondria and blunted by red light

Núñez-Álvarez, Claudia; Suárez-Barrio, Carlota; Olmo-Aguado, Susana del; Osborne, Neville N.

doi:10.1111/aos.13812

Cited by 37 publications

(34 citation statements)

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“…This energy takes the form of adenosine triphosphate (ATP) and it is produced by the electron flow carried out by the four complexes of the electron transport chain, which is governed by fundamental enzymes in the mitochondrial inner membrane [9,10]. Nevertheless, the presence of ROS can counteract the enzymes' functions by interrupting their reactions, thus, reducing ATP production and, as a result, leading to oxidative stress and cell death [11,12]. ROS output can be increased by a number of factors, including the aging process, smoking, and obesity, all of which contribute to the detriment of RPE cells [13].…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Role of PRGF on RPE Cells after Blue Light Insult as a Therapy for Neurodegenerative Diseases

Suárez-Barrio

Olmo-Aguado

García-Pérez

et al. 2020

IJMS

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Oxidative stress has a strong impact on the development of retinal diseases such as age-related macular degeneration (AMD). Plasma rich in growth factors (PRGF) is a novel therapeutic approach in ophthalmological pathologies. The aim of this study was to analyze the antioxidant effect of PRGF in retinal epithelial cells (EPR) in in vitro and ex vivo retinal phototoxicity models. In vitro analyses were performed on ARPE19 human cell line. Viability and mitochondrial status were assessed in order to test the primary effects of PRGF. GSH level, and protein and gene expression of the main antioxidant pathway (Keap1, Nrf2, GCL, HO-1, and NQO1) were also studied. Ex vivo analyses were performed on rat RPE, and HO-1 and Nrf2 gene and protein expression were evaluated. The results show that PRGF reduces light insult by stimulating the cell response against oxidative damage and modulates the antioxidant pathway. We conclude that PRGF’s protective effect could prove useful as a new therapy for treating neurodegenerative disorders such as AMD.

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Section: Introductionmentioning

confidence: 99%

“…This factor is the influence of blue light, i.e. short wavelength light of 400 to 500 nm [12]. This wavelength corresponds with the visible spectrum that reaches the retina and is one of the main fractions of new white illumination [15,16].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Role of PRGF on RPE Cells after Blue Light Insult as a Therapy for Neurodegenerative Diseases

Suárez-Barrio

Olmo-Aguado

García-Pérez

et al. 2020

IJMS

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“…To date, human RPE or ARPE-19 cells are used in in vitro AMD models with the frequently used inducers, Aβ 1-40 [ 30 ], Aβ 1-42 [ 31 ], hydrogen peroxide [ 32 ], or blue light [ 33 ]. Hydrogen peroxide or blue light are used for their ability to induce oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Lycium barbarum Polysaccharides on Pyroptosis-Associated Amyloid β1-40 Oligomers-Induced Adult Retinal Pigment Epithelium 19 Cell Damage

Yang

2020

IJMS

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Age-related macular degeneration (AMD) is a sight-threatening disease with limited treatment options. We investigated whether amyloid β1-40 (Aβ1-40) could cause pyroptosis and evaluated the effects of Lycium barbarum polysaccharides (LBP) on Aβ1-40 oligomers-induced retinal pigment epithelium 19 (ARPE-19) damage, which is an in vitro AMD model. Aβ1-40 oligomers verified by Western blot were added to ARPE-19 cells with or without 24 h LBP treatment. Aβ1-40 oligomers significantly decreased ARPE-19 cell viability with obvious morphological changes under light microscopy. SEM revealed swollen cells with a bubbling appearance and ruptured cell membrane, which are morphological characteristics of pyroptosis. ELISA results showed increased expression of IL-1β and IL-18, which are the final products of pyroptosis. LBP administration for 24 h had no toxic effects on ARPE-19 cells and improved cell viability and morphology while disrupting Aβ1-40 oligomerization in a dose-dependent manner. Furthermore, Aβ1-40 oligomers up-regulated the cellular immunoreactivity of pyroptosis markers including NOD-like receptors protein 3 (NLRP3), caspase-1, and membrane N-terminal cleavage product of GSDMD (GSDMD-N), which could be reversed by LBP treatment. Taken together, this study showed that LBP effectively protects the Aβ1-40 oligomers-induced pyroptotic ARPE-19 cell damages by its anti-Aβ1-40 oligomerization properties and its anti-pyroptotic effects.

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“…White light, which corresponds to visible light, is commonly formed by different fractions of short wavelengths, including the blue light spectrum. This short wavelength light reaches the retinal cells, causing a disruption in their molecular bases and finally damaging the cell structure [ 3 , 8 , 9 , 10 ]. The presence of risk factors, such as obesity, smoking, and aging, on top of pre-existing eye pathologies, can lead to a progressive decline in retinal cells’ integrity and, eventually, loss of vision [ 11 , 12 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Plasma Rich in Growth Factors Enhances Cell Survival after in Situ Retinal Degeneration

Suárez-Barrio

Olmo-Aguado

García-Pérez

et al. 2020

IJMS

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Purpose: The purpose of this study was to examine the effect of plasma rich in growth factors (PRGFs) under blue light conditions in an in vivo model of retinal degeneration. Methods: Male Wistar rats were exposed to dark/blue light conditions for 9 days. On day 7, right eyes were injected with saline and left eyes with PRGF. Electroretinography (ERG) and intraocular pressure (IoP) measurements were performed before and after the experiment. After sacrifice, retinal samples were collected. Hematoxylin and eosin staining was performed to analyze the structure of retinal sections. Immunofluorescence for brain-specific homeobox/POU domain protein 3A (Brn3a), choline acetyltransferase (ChAT), rhodopsin, heme oxygenase-1 (HO-1), and glial fibrillary acidic protein (GFAP) was performed to study the retinal conditions. Results: Retinal signaling measured by ERG was reduced by blue light and recovered with PRGF; however, IoP measurements did not show significant differences among treatments. Blue light reduced the expression for Brn3a, ChAT, and rhodopsin. Treatment with PRGF showed a recovery in their expressions. HO-1 and GFAP results showed that blue light increased their expression but the use of PRGF reduced the effect of light. Conclusions: Blue light causes retinal degeneration. PRGF mitigated the injury, restoring the functionality of these cells and maintaining the tissue integrity.

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Blue light negatively affects the survival of ARPE19 cells through an action on their mitochondria and blunted by red light

Cited by 37 publications

References 100 publications

Antioxidant Role of PRGF on RPE Cells after Blue Light Insult as a Therapy for Neurodegenerative Diseases

Antioxidant Role of PRGF on RPE Cells after Blue Light Insult as a Therapy for Neurodegenerative Diseases

The Effect of Lycium barbarum Polysaccharides on Pyroptosis-Associated Amyloid β1-40 Oligomers-Induced Adult Retinal Pigment Epithelium 19 Cell Damage

Plasma Rich in Growth Factors Enhances Cell Survival after in Situ Retinal Degeneration

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