BMP‐15 activity on in vitro development of collared peccary (Pecari tajacu Linnaeus, 1758) preantral follicles

Abstract

This study investigated the effects of BMP‐15 on the in vitro development of preantral follicles of collared peccaries. Ovarian fragments were cultured for 1 or 6 days in Tissue Culture Medium 199 (TCM199+) supplemented with BMP‐15 at rates of 0, 1, 25 or 50 ng/ml. The fragments were analysed histologically by evaluating follicular morphology, activation and growth as well as the potential for proliferation of granulosa cells. Our results show the addition of 25 ng/ml BMP‐15 in the medium provided the greatest percentage of normal follicles (79.67% ± 0.69) when compared to other treatments (p < .05); however, this result is similar to 1 ng/ml BMP‐15 (74.00% ± 1.90, p > .05). Moreover, 25 and 50 ng/ml of BMP‐15 promoted follicular activation. BMP‐15 supplements did not affect oocyte and follicular growth. All concentrations of BMP‐15 increased the number of nucleolus organizer regions (NORs) after 1 day of culture when compared to fresh fragments or the control samples (p < .05). However, at the end of the experiment, the number of NORs in follicles cultured in all treatments was higher than that observed in the fresh control (sample taken prior to culturing) (p > .05). In summary, the addition of 25 ng/ml BMP‐15 to the culture medium of collared peccary preantral follicles maintained a high number of morphologically healthy follicles and stimulated the activation of primordial follicles after 6 days in culture.

Biobanking and use of gonadal tissues - a promising strategy for conserving wildlife from the Caatinga biome

“… Gomes et al (2020) confirmed that the addition of 25 ng/mL of BMP-15 to the culture medium of collared peccary PAFs maintained a high number of morphologically healthy follicles and stimulated the activation of primordial follicles after 6 days in culture. In 2021, Campos et al observed that GDF-9, especially at a 200 ng/mL inclusion in the culture medium, was actively involved in the in vitro development of collared peccary PAFs.…”

The Role of BMP Signaling in Female Reproductive System Development and Function

“…It has been shown to be essential for oocyte development, ovulation rate, fertilization, and embryonic competence [ 56 ]. The treatment of collared peccary preantral follicles with BMP-15 stimulated the activation of primordial follicles and maintained a high number of morphologically healthy follicles [ 57 ]. Some breeds, such as sheep, are carriers of important genetic variants in the ovulation rate.…”

In vitro culture of red-rumped agouti preantral follicles enclosed in fresh and vitrified ovarian tissues using TCM199 plus different pFSH concentrations

“…Probably, other supplements need to compose the PAF culture media so that follicular activation can be observed in red-rumped agouti. This encouraging results present the perspective of improving the IVC system by trying other media as the MEM + ( Lima et al, 2018 ), other additives like the growth factors as the bone morphogenic protein 15 [BMP-15; Gomes et al (2020) ] or the growth differentiating factor-9 [GDF-9; Campos et al (2021) ], long-term culturing procedures ( Choi et al, 2008 ), culturing isolate PAFs ( Jamalzaei et al, 2016 ), and the use of three-dimensional systems ( Asgari et al, 2015 ).…”