BMP-2 functionalized PEDOT:PSS-based OECTs for stem cell osteogenic differentiation monitoring

Decataldo, Francesco; Druet, Victor; Pappa, Anna‐Maria; E, Tan; Savva, Achilleas; Pitsalidis, Charalampos; Inal, Sahika; Kim, Ji-Seon; Owens, Róisín M.; Iandolo, Donata

doi:10.1088/2058-8585/ab5bfc

Cited by 12 publications

(11 citation statements)

References 56 publications

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“…Furthermore, OECTs display high capacitance, low impedance, mechanical flexibility, chemical tunability, and optical transparency, making them ideal candidates for multiparametric sensing, simultaneous characterization with optical techniques and improved efficiency, and accuracy of data acquisition. [ 187,200,201 ] OECT devices have been used to study epithelial barrier formation and disruption, [ 202 ] stem cell differentiation, [ 203 ] and to detect analytes in human fluid samples for diagnostic purposes. [ 204 ] In the line of pathogen infection, the application of OECTs have been largely used to study food‐borne or bacterial infection of intestinal [ 205 ] and kidney cell lines.…”

Section: Future Directionsmentioning

confidence: 99%

In Vitro Models for Studying Respiratory Host–Pathogen Interactions

Barron

Sáez²,

Owens³

2021

Advanced Biology

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Respiratory diseases and lower respiratory tract infections are among the leading cause of death worldwide and, especially given the recent severe acute respiratory syndrome coronavirus‐2 pandemic, are of high and prevalent socio‐economic importance. In vitro models, which accurately represent the lung microenvironment, are of increasing significance given the ethical concerns around animal work and the lack of translation to human disease, as well as the lengthy time to market and the attrition rates associated with clinical trials. This review gives an overview of the biological and immunological components involved in regulating the respiratory epithelium system in health, disease, and infection. The evolution from 2D to 3D cell biology and to more advanced technological integrated models for studying respiratory host–pathogen interactions are reviewed and provide a reference point for understanding the in vitro modeling requirements. Finally, the current limitations and future perspectives for advancing this field are presented.

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Section: Future Directionsmentioning

confidence: 99%

In Vitro Models for Studying Respiratory Host–Pathogen Interactions

Barron

Sáez²,

Owens³

2021

Advanced Biology

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“…A similar biosensor based on OECTs with gate electrodes modified with MIP film were developed and used for the chiral recognition of amino acid enantiomers (d/l-tryptophan and d/l-tyrosine) with a detection limit of 2 × 10 −9 m. [156] OECTs can also act as metabolite transducers for the detection of various enzymes, such as glucose, uric acid (UA), dopamine (DA), cholesterol, and lactic acid, which are vital for clinical diagnostics, health monitoring, food safety, environmental monitoring, etc. [157][158][159][160][161] For the detection of metabolites, the sensing mechanism owes to the selective interaction of a redox enzyme with the metabolite molecules, which leads to the transfer of an electron to the gate of OECT. The channel current change depends on the concentration of the metabolite in electrolyte.…”

Section: Biological Sensorsmentioning

confidence: 99%

Recent Technological Advances in Fabrication and Application of Organic Electrochemical Transistors

Chen

Surendran

et al. 2020

Adv Materials Technologies

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The classical organic semiconducting material used for OECTs is an ionomer mixture called poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), comprising of a conjugated PEDOT phase and a deprotonated PSS phase. Holes in the PEDOT phase is compensated by the negatively charged sulfonate ions in the PSS phase, making the PEDOT phase conducting. In the absence of a gate bias (V GS = 0 V), the flow of mobile holes within the polymer maintains a hole current when a drain voltage (V DS) is applied (ON state). By applying a positive gate voltage (V GS > 0 V), cations from the electrolyte are injected into the channel and PSS anions are charge compensated, initiating the dedoping of PEDOT, reducing the hole density and thus lowering the drain current (OFF state). Such mode of operation is known as depletion mode; the OECT is ON in the absence of a gate bias. It is also possible to operate the OECT in accumulation mode if the conjugated polymer does not consist of any native dopants. Accumulatedmode OECT is typically in the OFF state and application of a negative gate voltage leads to injection of anions into the semiconducting polymer, initiating an electrochemically driven hole accumulation in the channel, leading to the ON state. Recently, naphthalene diimides (NDIs)-based polymers, [15] ladder-type polymers such as poly(benzimidazobenzophenanthro-line) (BBL), [16] fullerene derivatives with glycolated side chains, [17] and random copolymer with glycol side chain such as P-90 [9,18] were reported to be promising n-type materials to develop accumulation mode OECTs with stable operation in aqueous environments. Another class of promising p-type materials for accumulation mode OECTs is semiconducting polymers based on benzodithiophene or bithiophene core with glycol side chains, [19,20] glycolated thiophene (g2T-T) polymers, [21] poly(3hexylthiophene) (P3HT), [22,23] conjugated polyelectrolytes based on poly(6-(thiophene-3-yl)hexane-1-sulfonate) tetrabutylammonium (PTHS), [24] polythiophene derivative with ethylene glycolbased side chains (P3MEEMT), [12] and poly(6-(thiophen-3-yl) hexane-1-sulfonate) tetrabutylammonium (PTHS:TEA). [25] Generally, the material must have simultaneously an aggregated, interconnected structure that supports efficient hole transport, and an amorphous phase to hydrate, and thus enable the transport of ions. The in-depth introduction on active materials for OECTs can be found in several review papers. [26-30] The key feature of OECT is that the transition of doping/ dedoping states occurs over the entire channel volume, as Organic electrochemical transistors (OECTs), where conjugated polymers undergo doping/dedoping from an electrolyte, are widely studied for applications ranging from switching elements, artificial synapses to transducers for biological sensing. The concurrent transport of electronic and ionic charges within the channel provide OECTs with excellent amplification capability and efficient ion-to-electron transduction at low operating voltages (<1 V). Herein, the la...

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“…PEDOT based devices have been successfully employed in bioelectronics applications as electrode arrays to record brain signals 47 , as cell scaffolds to stimulate and measure cell growth [48][49][50][51] , as biosensors to record biomarkers 22,[52][53][54] , and as active materials for drug delivery devices 55 .…”

Section: Organic Bioelectronicsmentioning

confidence: 99%

“…using various sensing units such as antibodies 51,[94][95][96] , oligonucleotide strands 111,112 , or membranes 113 .…”

Section: Oect As Biosensormentioning

confidence: 99%

Organic Bioelectronic Devices for Selective Biomarker Sensing : Towards Integration with Living Systems

Diacci

2021

Linköping Studies in Science and Technology. Dissertations

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Inorganic materials have been the main players of the semiconductor industry for the past forty years. However, there has been a continuous interest and growth in the research and in the application of organic semiconductors (OSCs) as active materials in electronic devices, due to the possibility to process these materials at low temperature on flexible substrates, fabricate them on large-area, and upscale their fabrication using cost-effective strategies such LIST OF PAPERS AND CONTRIBUTION Paper ILabel free urea biosensor based on organic electrochemical transistors.

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BMP-2 functionalized PEDOT:PSS-based OECTs for stem cell osteogenic differentiation monitoring

Cited by 12 publications

References 56 publications

In Vitro Models for Studying Respiratory Host–Pathogen Interactions

In Vitro Models for Studying Respiratory Host–Pathogen Interactions

Recent Technological Advances in Fabrication and Application of Organic Electrochemical Transistors

Organic Bioelectronic Devices for Selective Biomarker Sensing : Towards Integration with Living Systems

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