BMP2 and BMP6 control p57Kip2 expression and cell growth arrest/terminal differentiation in normal primary human epidermal keratinocytes

Gosselet, Fabien; Magnaldo, Thierry; Culerrier, Raphaël; Sarasin, Alain; Ehrhart, Jean-Claude

doi:10.1016/j.cellsig.2006.09.006

Cited by 51 publications

(50 citation statements)

References 40 publications

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“…The coordinated expression of the CIP/KIP members promotes keratinocyte differentiation (162,163). During pancreatic development p57 KIP2 controls both self-renewal and cell cycle exit of pancreatic progenitors (18).…”

Section: Kip1mentioning

confidence: 99%

p57KIP2: “Kip”ing the Cell under Control

Pateras

Apostolopoulou

Niforou

et al. 2009

Molecular Cancer Research

144

163

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Section: Kip1mentioning

confidence: 99%

p57KIP2: “Kip”ing the Cell under Control

Pateras

Apostolopoulou

Niforou

et al. 2009

Molecular Cancer Research

144

163

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“…BMP2, which belongs to the transforming growth factorbeta family, inhibits the proliferation of interfollicular epidermal keratinocytes coupled with induction of their terminal differentiation. 22) BMP2 was shown to induce a proinflammatory endothelial phenotype, such as increasing the production of reactive oxygen species and activating the transcriptional factor NF-kB, which induced the expression of IL-1a, IL-1b and other inflammatory genes. 23) Expression of BMP2 was reported to be induced by the proinflammatory cytokine TNF-a and the transcriptional factor NF-kB.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Skin Irritation Using a DNA Microarray on a Reconstructed Human Epidermal Model

Niwa

Nagai

Oike

et al. 2009

Biological & Pharmaceutical Bulletin

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To avoid the need to use animals to test the skin irritancy potential of chemicals and cosmetics, it is important to establish an in vitro method based on the reconstructed human epidermal model. To evaluate skin irritancy efficiently and sensitively, we determined the gene expression induced by a topically-applied mild irritant sodium dodecyl sulfate (SDS) in a reconstructed human epidermal model LabCyte TM EPI-MODEL (LabCyte) using a DNA microarray carrying genes that were related to inflammation, immunity, stress and housekeeping. The expression and secretion of IL-1a a in reconstructed human epidermal culture is known to be induced by irritation. We detected the induction of IL-1a a expression and its secretion into the cell culture medium by treatment with 0.075% SDS for 18 h in LabCyte culture using DNA microarray, quantitative reverse-transcription polymerase chain reaction (RT-PCR) and ELISA. DNA microarray analysis indicated that the expression of 10 of the 205 genes carried on the DNA microarray was significantly induced in a LabCyte culture by 0.05% or 0.075% SDS irritation for 18 h. RT-PCR analysis confirmed that SDS treatment significantly induced the expressions of interleukin-1 receptor antagonist (IL-1RN), FOS-like antigen 1 (FOSL1), heat shock 70 kDa protein 1A (HSPA1) and myeloid differentiation primary response gene (88) (MYD88), as well as the known marker genes for irritation IL-1b b and IL-8 in a LabCyte culture. Our results showed that a DNA microarray is a useful tool for efficiently evaluating mild skin irritation using a reconstructed human epidermal model.

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“…The data connected with Wnt, Notch, and BMP pathways in the IFE are more limited. It was suggested that BMP changes the fate of proliferating stem cells by transiting amplifying cells toward differentiation, which is coupled with the overexpression of K10 and involucrin (47). It was shown that the dermal BMP-FGF axis, activated by Wnt of epidermal origin, is required for proper epidermal stratification, while simultaneously, BMP4 promotes epithelial growth in a non-cell-autonomous manner by activating basal keratinocytes through FGF7 and FGF10 signaling (4).…”

Section: Foxn1 Involvement In Signaling Pathwaysmentioning

confidence: 99%

FOXN1 Transcription Factor in Epithelial Growth and Wound Healing

Grabowska

Wilanowski

2017

Molecular and Cellular Biology

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FOXN1 is a prodifferentiation transcription factor in the skin epithelium. Recently, it has also emerged as an important player in controlling the skin wound healing process, as it actively participates in reepithelialization and is thought to be responsible for scar formation. FOXN1 positivity is also a feature of pigmented keratinocytes, including nevi, and FOXN1 is an attribute of benign epithelial tumors. The lack of FOXN1 favors the skin regeneration process displayed by nude mice, pointing to FOXN1 as a switch between regeneration and reparative processes. The stem cell niche provides a functional source of cells after the loss of tissue following wounding. The involvement of prodifferentiation factors in the regulation of this pool of stem cells is suggested. However, the exact mechanism is still under question, and we speculate that the FOXN1 transcription factor is involved in this process. This review analyzes the pleiotropic effects of FOXN1 in the skin, its function in the tumorigenesis process, and its potential role in depletion of the stem cell niche after injury, as well as its suggested mechanistic role, acting in a cell-autonomous and a non-cell-autonomous manner during skin self-renewal.

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BMP2 and BMP6 control p57Kip2 expression and cell growth arrest/terminal differentiation in normal primary human epidermal keratinocytes

Cited by 51 publications

References 40 publications

p57KIP2: “Kip”ing the Cell under Control

p57KIP2: “Kip”ing the Cell under Control

Evaluation of the Skin Irritation Using a DNA Microarray on a Reconstructed Human Epidermal Model

FOXN1 Transcription Factor in Epithelial Growth and Wound Healing

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