BMP7 and EREG Contribute to the Inductive Potential of Dental Mesenchyme

Gao, Bo; Zhou, Xin; Zhou, Xuedong; Pi, Caixia; Xu, Ruoshi; Wan, Mei-Hua; Yang, Jing; Zhou, Yue; Liu, Chengcheng; Sun, Jing; Zhang, Yan; Zheng, Liwei

doi:10.1038/srep09903

Cited by 13 publications

(14 citation statements)

References 57 publications

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“…In the present study, cells were treated with 100 ng/mL of rh BMP7 [ 118 , 119 , 120 ], which is less than the concentration of BMP7 used in other studies on corneal keratocytes and epithelial cells [ 34 , 121 ]. No toxic effects on epithelial cells and SFs were observed while using rhBMP7.…”

Section: Methodsmentioning

confidence: 99%

Role of Bone Morphogenetic Protein 7 (BMP7) in the Modulation of Corneal Stromal and Epithelial Cell Functions

Kowtharapu

Prakasam

Murín

et al. 2018

IJMS

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In the cornea, healing of the wounded avascular surface is an intricate process comprising the involvement of epithelial, stromal and neuronal cell interactions. These interactions result to the release of various growth factors that play prominent roles during corneal wound healing response. Bone morphogenetic proteins (BMPs) are unique multi-functional potent growth factors of the transforming growth factor-beta (TGF-β) superfamily. Treatment of corneal epithelial cells with substance P and nerve growth factor resulted to an increase in the expression of BMP7 mRNA. Since BMP7 is known to modulate the process of corneal wound healing, in this present study, we investigated the influence of exogenous rhBMP7 on human corneal epithelial cell and stromal cell (SFs) function. To obtain a high-fidelity expression profiling of activated biomarkers and pathways, transcriptome-wide gene-level expression profiling of epithelial cells in the presence of BMP7 was performed. Gene ontology analysis shows BMP7 stimulation activated TGF-β signaling and cell cycle pathways, whereas biological processes related to cell cycle, microtubule and intermediate filament cytoskeleton organization were significantly impacted in corneal epithelial cells. Scratch wound healing assay showed increased motility and migration of BMP7 treated epithelial cells. BMP7 stimulation studies show activation of MAPK cascade proteins in epithelial cells and SFs. Similarly, a difference in the expression of claudin, Zink finger E-box-binding homeobox 1 was observed along with phosphorylation levels of cofilin in epithelial cells. Stimulation of SFs with BMP7 activated them with increased expression of α-smooth muscle actin. In addition, an elevated phosphorylation of epidermal growth factor receptor following BMP7 stimulation was also observed both in corneal epithelial cells and SFs. Based on our transcriptome analysis data on epithelial cells and the results obtained in SFs, we conclude that BMP7 contributes to epithelial-to-mesenchymal transition-like responses and plays a role equivalent to TGF-β in the course of corneal wound healing.

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Section: Methodsmentioning

confidence: 99%

Role of Bone Morphogenetic Protein 7 (BMP7) in the Modulation of Corneal Stromal and Epithelial Cell Functions

Kowtharapu

Prakasam

Murín

et al. 2018

IJMS

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“…Additionally, EREG is shown to assist in the proliferation, repair, or regeneration (or a combination of these) in liver, colon, and salivary gland acinar cells 39 – 42 . EREG also plays a role in odontogenesis by enhancing proliferation of dental apical papilla stem cells and inducing oral epithelial cell differentiation by dental papilla cells 43 .…”

Section: Aspects Of Individual Ligandsmentioning

confidence: 99%

EGF receptor ligands: recent advances

2016

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Seven ligands bind to and activate the mammalian epidermal growth factor (EGF) receptor (EGFR/ERBB1/HER1): EGF, transforming growth factor-alpha (TGFA), heparin-binding EGF-like growth factor (HBEGF), betacellulin (BTC), amphiregulin (AREG), epiregulin (EREG), and epigen (EPGN). Of these, EGF, TGFA, HBEGF, and BTC are thought to be high-affinity ligands, whereas AREG, EREG, and EPGN constitute low-affinity ligands. This focused review is meant to highlight recent studies related to actions of the individual EGFR ligands, the interesting biology that has been uncovered, and relevant advances related to ligand interactions with the EGFR.

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“…BMP7 protein, a crucial factor in developmental abnormalities and diseases, had a wide range of functions [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. The absence of BMP7 was a vital cause of perinatal mortality with kidney stunting and malformations.…”

Section: Introductionmentioning

confidence: 99%

MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e

Liu

Zhou

Yuan

et al. 2015

IJMS

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Accumulating evidence demonstrated that miRNAs are highly involved in kidney fibrosis and Epithelial-Eesenchymal Transition (EMT), however, the mechanisms of miRNAs in kidney fibrosis are poorly understood. In this work, we identified that miR542-3p could promote EMT through down-regulating bone morphogenetic protein 7 (BMP7) expression by targeting BMP7 3′UTR. Firstly, real-time PCR results showed that miR542-3p was significantly up-regulated in kidney fibrosis in vitro and in vivo. Moreover, Western blot results demonstrated that miR542-3p may promote EMT in the NRK52e cell line. In addition, we confirmed that BMP7, which played a crucial role in anti-kidney fibrosis and suppressed the progression of EMT, was a target of miR542-3p through Dual-Luciferase reporter assay, as did Western blot analysis. The effects of miR542-3p on regulating EMT could also be suppressed by transiently overexpressing BMP7 in NRK52e cells. Taken together, miR542-3p may be a critical mediator of the induction of EMT via directly targeting BMP7.

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BMP7 and EREG Contribute to the Inductive Potential of Dental Mesenchyme

Cited by 13 publications

References 57 publications

Role of Bone Morphogenetic Protein 7 (BMP7) in the Modulation of Corneal Stromal and Epithelial Cell Functions

Role of Bone Morphogenetic Protein 7 (BMP7) in the Modulation of Corneal Stromal and Epithelial Cell Functions

EGF receptor ligands: recent advances

MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e

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