BMSCs laden injectable amino-diethoxypropane modified alginate-chitosan hydrogel for hyaline cartilage reconstruction

Wan, Wenbing; Li, Qingtao; Gao, Haiyun; Ge, Liangpeng; Liu, Yuqing; Zhong, Wen; Ouyang, Jun; Xing, Malcolm

doi:10.1039/c4tb01394h

Cited by 32 publications

(34 citation statements)

References 56 publications

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“…of studies using the score (%)ReferencesHistology scores111 (100%)[49, 54–102, 134, 150–154, 160, 161, 272–324]International Cartilage Repair Society Score26 (23%)[49, 60, 61, 63, 66, 69, 72, 74, 79, 89, 92, 94, 98, 99, 272, 282, 283, 289, 305, 306, 310, 313, 314, 316, 319, 324]Wakitani score21 (19%)[58, 62, 67, 68, 72, 73, 80, 82, 97, 151, 273, 277, 279, 284, 285, 290, 299, 304, 310, 321]O’Driscoll score2018%[49, 71, 81, 84, 85, 93, 100, 160, 272, 276, 290, 296–298, 302, 306, 308, 313, 314, 322]Functional scores/mechanical11 (10%)[55, 57, 62, 67, 69, …”

Section: Resultsmentioning

confidence: 99%

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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BackgroundThe management of articular cartilage defects presents many clinical challenges due to its avascular, aneural and alymphatic nature. Bone marrow stimulation techniques, such as microfracture, are the most frequently used method in clinical practice however the resulting mixed fibrocartilage tissue which is inferior to native hyaline cartilage. Other methods have shown promise but are far from perfect. There is an unmet need and growing interest in regenerative medicine and tissue engineering to improve the outcome for patients requiring cartilage repair. Many published reviews on cartilage repair only list human clinical trials, underestimating the wealth of basic sciences and animal studies that are precursors to future research. We therefore set out to perform a systematic review of the literature to assess the translation of stem cell therapy to explore what research had been carried out at each of the stages of translation from bench-top (in vitro), animal (pre-clinical) and human studies (clinical) and assemble an evidence-based cascade for the responsible introduction of stem cell therapy for cartilage defects.Main body of abstractThis review was conducted in accordance to PRISMA guidelines using CINHAL, MEDLINE, EMBASE, Scopus and Web of Knowledge databases from 1st January 1900 to 30th June 2015. In total, there were 2880 studies identified of which 252 studies were included for analysis (100 articles for in vitro studies, 111 studies for animal studies; and 31 studies for human studies). There was a huge variance in cell source in pre-clinical studies both of terms of animal used, location of harvest (fat, marrow, blood or synovium) and allogeneicity. The use of scaffolds, growth factors, number of cell passages and number of cells used was hugely heterogeneous.Short conclusionsThis review offers a comprehensive assessment of the evidence behind the translation of basic science to the clinical practice of cartilage repair. It has revealed a lack of connectivity between the in vitro, pre-clinical and human data and a patchwork quilt of synergistic evidence. Drivers for progress in this space are largely driven by patient demand, surgeon inquisition and a regulatory framework that is learning at the same pace as new developments take place.

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Section: Resultsmentioning

confidence: 99%

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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“…The sample was cut with scissors into 15×10 mm 2 rectangular shape and the thickness was measured with a vernier caliper, then the sample was placed between two crossheads, resulting in specimens dimension of 10×10×0.1 mm 3 , and the crosshead speed was set to 10 mm/min. Data, including stress (in MPa) and elongation (%), were automatically calculated by the Instron's Bluehill 2 software (Instron, High Wycombe, UK).…”

Section: Pcl Nanofibrous Mesh Preparation and Tensile Testmentioning

confidence: 99%

Polycaprolactone nanofibrous mesh reduces foreign body reaction and induces adipose flap expansion in tissue engineering chamber

Luo¹,

He²,

Chang³

et al. 2016

IJN

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“…8–10 In many biomedical applications, including injectable tissue engineering, 11,12 minimally invasive drug delivery, 13 wound dressing, 14,15 materials that can form in situ hydrogels have considerable advantages. 16,17 In this technique, a premixed solution containing cross-linkable polymers, drugs, cells and/or other ingredient, is injected to fill native or potential cavities, and forms a matrix in vivo conforming to different shapes, which may be difficult to prefabricate.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of in situ forming anionic hydrogel as vitreous substitutes

Liang

Struckhoff

et al. 2016

J. Biomed. Mater. Res.

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The natural vitreous is a biological hydrogel consisting primarily of a collagen and anionic hyaluronate. It is surgically removed in many ocular diseases and replaced with fluids, gases, or silicone oils. We have been interested in developing synthetic hydrogels as vitreous substitutes. In this study, we combined the stiffness and hydrophobicity of polymethacrylamide (PMAM) and the anionic nature of poly-methacrylate (PMAA) to make copolymers that would mimic the natural vitreous. We used bis-methacryloyl cystamine (BMAC) to introduce thiol groups for reversible crosslink. The Mn of copolymers ranged from ~100 k to ~200 k Da (polydis-peristy index of 1.47–2.63) and their composition as determined by titration, 1H NMR and disulfide test were close to the feed ratio. The reactivities of monomers were as follows: MAM > MAA ~ BMAC. Copolymers with higher MAA contents gelled faster, swelled more, and had higher storage modulus (1.5 to 100 Pa) comparable to that of the natural vitreous. We evaluated the biocompatibility of copolymers by electric cell-substrate impedance sensing (ECIS) using human retinal pigment epithelial cells, primary porcine retinal pigmented epithelial cells, human microvascular endothelial cells adult dermis, and a fibroblast line 3T3. The biocompatibility decreases as the content of BMAC increases.

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BMSCs laden injectable amino-diethoxypropane modified alginate-chitosan hydrogel for hyaline cartilage reconstruction

Abstract: We developed an injectable hydrogel composed of amino-diethoxypropane modified alginate and chitosan, and also investigated bone marrow mesenchy + mal stromal cells (BMSCs) laden hydrogel for cartilage reconstruction in vitro and in vivo.

Cited by 32 publications

References 56 publications

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

Polycaprolactone nanofibrous mesh reduces foreign body reaction and induces adipose flap expansion in tissue engineering chamber

Synthesis and characterization of in situ forming anionic hydrogel as vitreous substitutes

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