Boar semen cryopreserved with trehalose-containing liposomes: disaccharide determination and rheological behaviour

Abstract

This study aimed to detect intracellular trehalose in boar sperm that were cryopreserved with liposomes and conduct an analysis of its effects on some characteristics of thawed sperm, including rheological properties. First, soybean lecithin cholesterol-based liposomes were produced and characterized in the presence of 300 mM trehalose. Next, semen samples were frozen in two freezing media: a control medium with 300 mM trehalose and an experimental medium supplemented with 300 mM trehalose and 10% liposomes, both of which were thawed and then studied to ascertain their integrity, motility, rheological response, and trehalose quantities by testing two methods of spermatic lysis via high-performance liquid chromatography with an evaporative light-scattering detector (HPLC-ELSD). The results found spherical liposomes measuring 357 nm that were relatively stable in an aqueous medium and had an entrapment efficiency of 73%. An analysis of the cryopreserved ejaculates showed that their viability and motility did not significantly differ between groups (P > 0.05). The viscous response of the samples was influenced by the extracellular medium rather than by the freezing-thawing process, which resulted in a loss of interaction between the cells and cryoprotectants. Finally, intracellular trehalose levels were determined using HPLC-ELSD, with no differences observed (P > 0.05) when comparing both sperm lysis methods. The use of liposomes with trehalose appears to be a promising option for boar semen cryopreservation, with a marked effect on rheological properties. The proposed HPLC-ELSD method was effective for measuring trehalose in cryopreserved cell samples.

Effects of propolis-loaded nanoliposomes fortification in extender on buffalo semen cryopreservation

“…Liposomes are spherical vesicles consisting of biodegradable and biocompatible phospholipids representing biological membranes 1 , 3 . Nanoliposomes (NLPs) are one of the utmost attractive nanocarriers, with very small particle, owing to their benefits such as biocompatibility, flexibility, amphiphilicity, efficient cellular uptake, non-toxicity, and non-immunogenicity 7 , 8 . Moreover, NLPs have been applied for the encapsulation of natural pigments, enzymes, fatty acids, vitamins, plant extracts and their essential oils 7 , 9 .…”

“…Nanoliposomes (NLPs) are one of the utmost attractive nanocarriers, with very small particle, owing to their benefits such as biocompatibility, flexibility, amphiphilicity, efficient cellular uptake, non-toxicity, and non-immunogenicity 7 , 8 . Moreover, NLPs have been applied for the encapsulation of natural pigments, enzymes, fatty acids, vitamins, plant extracts and their essential oils 7 , 9 . Recently, natural nano molecules are reported to have several biological activities and are widely fortified in freezing media to enhance semen cryopreservation 2 due to their safety, and therapeutic possessions.…”

“…Al-Nawab et al 10 found that the using of propolis extract (0.5–1 mg/ mL) has a considerable consequence on in vitro enhancement of sperm motility and health in infertile patient via maintaining the sperm membrane and DNA integrity as well as support the mitochondria function. Many studies reported that different natural compounds coated with liposome showed significant influence on cryopreserved semen function in animals 3 , 7 – 9 . However, the use of propolis-loaded nanoliposomes (PRNL) fortified with freezing media of buffalo semen has previously been unexplored.…”

Low concentrations of soybean lecithin nanoparticles had a positive impact on Holstein bulls' cryopreserved semen

“…Artificial insemination is a technique used in cattle breeding to accomplish genetic enhancements (Mendoza‐Viveros et al., 2022). The process of preserving semen using specialized freezing media, which frequently includes cryoprotectants like trehalose and liposomes, is known as cryopreservation (Lassen et al., 2022).…”