BODIPY-based Fluorescent Probe for the Detection of Cysteine in Living Cells

Abstract

Cysteine (Cys), as one of the important amino acids, plays a vital role in various physiological and pathological processes. Hence, it is meaningful to develop a convenient and sensitive detection method. Herein, a novel BODIPY-based fluorescent probe (BDP-DM) was developed, which had a higher selectivity for Cys than other amino acids, including homocysteine (Hcy) and glutathione (GSH). Ultimately, we concluded that the BDP-DM probe could be used to successfully detected intracellular Cys in living HeLa cells.

Fluorescent probes based on nucleophilic aromatic substitution reactions for reactive sulfur and selenium species: Recent progress, applications, and design strategies

“…There are many small aromatic molecules that can bind to fluorophores and are attacked by RSS or RSeS. Below, we describe some unusual cases [243] , [244] , [245] , [246] .…”

Molecular Imaging

“…Intracellular Cys could be detected by fluorescent imaging with BDP-DM. 7 Other research groups have also attempted to distinguish intracellular biothiols using different probes. Men et al developed a cyanine-based near-infrared fluorescent probe (Cy-S-Py) that could sense Cys and Hcy (but not GSH) at a detection limit of 0.17 μM.…”