Body Fluid Cell Counts by Automated Methods

Sandhaus, Linda M.

doi:10.1016/j.cll.2014.10.003

Cited by 39 publications

(71 citation statements)

References 38 publications

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“…However, each laboratory should verify these advantages for each liquid type (12). Hybrid hemocytometers with modules for biological fluid analysis are being developed to provide benefits from automation, including accuracy, precision, and efficiency.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer

Aguadero

Cano-Corres

Berlanga

et al. 2017

Cytometry Part B Clinical

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Section: Discussionmentioning

confidence: 99%

Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer

Aguadero

Cano-Corres

Berlanga

et al. 2017

Cytometry Part B Clinical

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“…Cell counting and classification in body fluids (BFs; ascitic [AF], pleural [PF], synovial [SF], and cerebrospinal [CSF]) has become very popular in recent times due to enhanced availability of new commercial instrumentation . Carried out using suitable analyzers, automated cell count can now be regarded as a useful screening, since is adequately precise, may substantially reduce the turnaround time (TAT), is available 24 hours a day, and allows better standardization of the analytical process …”

Section: Introductionmentioning

confidence: 99%

Lack of harmonization in high fluorescent cell automated counts with body fluids mode in ascitic, pleural, synovial, and cerebrospinal fluids

Buoro

Seghezzi

Dominoni

et al. 2019

Int J Lab Hematology

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Introduction Cellular analysis in body fluids (BFs) provides important diagnostic information in various pathological settings. This study was hence aimed at comparing automated cell count obtained with Mindray BC‐6800 (BC‐BF) vs Sysmex XN‐series (XN‐BF) and evaluating other quantitative and qualitative information provided by these analyzers in ascitic (AF), pleural (PF), synovial (SF), and cerebrospinal (CSF) fluids. Methods Three hundred and fifty‐one samples (99 AFs, 45 PFs, 75 SFs, and 132 CSFs) were analyzed in parallel with BC‐BF, XN‐BF, and optical microscopy (OM). The study also included the assessment of diagnostic agreement among BC‐BF, XN‐BF, and OM. Results The comparison of BC‐BF vs XN‐BF yielded slopes of Passing and Bablok regression always comprised between 0.9 and 1.0 except for EO‐BF and HF‐BF, whilst the intercepts ranged from −0.4 for MN‐BF and 12.0 for PMN‐BF. The bias was comprised between −103.3% and 67.1% for HF‐BF and EO‐BF, respectively. A significant bias was found for TC‐BF, WBC‐BF, HF‐BF (negative bias) and for PMN‐BF and EO‐BF (positive bias). The agreement (Cohen's kappa) between XN‐BF and BC‐BF was always ≥0.7, ranging between 0.87 in CSFs and 0.94 in AFs, and that with OM was similar (ie, 0.85 and 0.96). Conclusion The cytometric analysis of BF samples using BC‐BF and XN‐BF is clinically favorable when appropriately combined with OM. Quantitative and qualitative parameters displayed optimal agreement, whilst instrument‐specific cut‐offs should be defined and implemented for HF‐BF and EO‐BF. Further efforts should be made for achieving better harmonization in cytometric analysis of BF samples.

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“…Traditionally, exfoliative cytology is performed by microscopic examination, which is time‐consuming, has low sensitivity, and may lead to a delay in the early diagnosis of patients. Recently, hematology analyzers for assessing BF have received much attention because they can improve laboratory efficiency and cost‐effectiveness, A few studies have been dedicated to assessing the performance of automated hematology analyzers and comparing the correlation between the automated method and manual microscopic method, These studies showed that the automated methods had good agreement with the manual microscopic method and had better precision. The body fluid mode (BF mode) introduced by the Sysmex XN9000 can automatically count the red blood cells, white blood cells, mononuclear cells (MN), and polymorphonuclear cells (PMN) in serosal cavity effusions, including pleural effusion, cerebrospinal fluid, and articular fluid.…”

Section: Introductionmentioning

confidence: 99%

The diagnostic ability of high‐fluorescent cells combined with carcinoembryonic antigen for malignant pleural effusion

WenJing

Zhao

Shen

et al. 2019

Int J Lab Hematology

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Introduction High‐fluorescent cells (HFCs) that are detected with an automated hematology analyzer may be useful for the detection of tumor cells; however, the diagnostic ability of HFCs for differentiating malignant pleural effusion is limited. The aim of this study was to investigate the diagnostic value of the combined detection of HFCs with the tumor marker carcinoembryonic antigen (CEA) for the identification of malignant hydrothorax. Methods A total of 115 pleural effusions were collected. HFCs, including the relative counts (HF‐BF%) and absolute counts (HF‐BF#), were analyzed using the BF mode of a Sysmex XN9000 hematology analyzer. Simultaneously, the CEA level from the same patient was measured by an electrochemiluminescence method. Receiver operating characteristic (ROC) curve analysis was employed to evaluate the diagnostic accuracy of HFCs separately or combined with CEA analysis for malignant diseases. Results The levels of HF‐BF#, HF‐BF%, and CEA in the malignant effusion group were significantly higher than those in the benign control group. The diagnostic value of the HF‐BF# and HF‐BF% for malignant pleural effusion was low to moderate, and the area under the curve (AUC) was 0.663 and 0.715, respectively. The CEA detection showed a moderate diagnostic ability, and the AUC was 0.832. The AUC for the combined methods was 0.860 and 0.890, respectively. The cutoff levels of the HF‐BF#, HF‐BF%, and CEA levels were 29.5 × 106/L, 5.6/100 WBC, and 4.795 ng/mL, respectively. Conclusions The combined detection of high‐fluorescent cells with the BF mode and CEA testing may be a good indication for malignancy.

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Body Fluid Cell Counts by Automated Methods

Cited by 39 publications

References 38 publications

Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer

Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer

Lack of harmonization in high fluorescent cell automated counts with body fluids mode in ascitic, pleural, synovial, and cerebrospinal fluids

The diagnostic ability of high‐fluorescent cells combined with carcinoembryonic antigen for malignant pleural effusion

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