Body size-dependent interspecific tolerance to cadmium and their molecular responses in the marine rotifer Brachionus spp.

Kang, Hye-Min; Lee, Jin-Sol; Lee, Young Hwan; Kim, Min‐Sub; Park, Heum Gi; Jeong, Chang‐Bum; Lee, Jae‐Seong

doi:10.1016/j.aquatox.2018.10.020

Cited by 31 publications

(4 citation statements)

References 43 publications

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“…IBA3 was significantly more tolerant to cadmium than MRS10 (210.5 mg L −1 and 130.8 mg L −1 , respectively) (Table 1 ). In a study by Kang et al ( 2019 ), the LC 50 value obtained for neonates of B. koreanus (142.69 mg L −1 ) was similar to the one obtained for MRS10 but for only 6 h of exposure. This seems to indicate that MRS10 and IBA3 have higher tolerance to cadmium than the organisms studied by Kang et al ( 2019 ).…”

Section: Discussionsupporting

confidence: 71%

“…In a study by Kang et al ( 2019 ), the LC 50 value obtained for neonates of B. koreanus (142.69 mg L −1 ) was similar to the one obtained for MRS10 but for only 6 h of exposure. This seems to indicate that MRS10 and IBA3 have higher tolerance to cadmium than the organisms studied by Kang et al ( 2019 ). These authors observed that body size correlated inversely with metabolic rate, which is a key factor for cadmium bioaccumulation.…”

Section: Discussionsupporting

confidence: 71%

“…This is particularly relevant for B. plicatilis since, having once been considered a species with morphological variability, more recent taxonomic and genetic studies revealed it is in fact a species complex (Serra and Fontaneto 2017 ). Studies have been conducted to better understand the differences among biotypes, addressing parameters such as growth and reproduction (Gómez et al 1997 ; Granada et al 2022 ; Hagiwara et al 1995 ), morphology (Ciros-Pérez et al 2001 ; Hwang et al 2013 ), lifespan (Gribble et al 2014 ; Kaneko et al 2011 ; Snare et al 2013 ), response to toxicants (Han et al 2023 ; Kang et al 2019 ), and others.…”

Section: Introductionmentioning

confidence: 99%

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Swimming behaviour as an alternative endpoint to assess differences in abiotic stress sensitivities between strains of Brachionus koreanus (Rotifera: Monogononta)

Granada

Lemos

Bossier

et al. 2023

Environ Sci Pollut Res

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Brachionus plicatilis is a cosmopolitan rotifer used as a model organism in several research areas and as live food in aquaculture. Being a species complex, responses to stressors vary even among strains of the same species and, thus, the responses of one species are not representative of the whole complex. This study aimed to address the effects of extreme salinity ranges, and different concentrations of hydrogen peroxide, copper, cadmium, and chloramphenicol, in two strains of B. koreanus (MRS10 and IBA3) from B. plicatilis species complex, by assessing effects on their survival and swimming capacity. Neonates (0–4 h old) were exposed to the stressors in 48 well-microplates, for 24 and 6 h, to evaluate lethal and behavioural effects, respectively. Tested conditions of chloramphenicol did not show any effects on rotifers. The behavioural endpoint showed to be particularly sensitive to assess the effects of high salinity, hydrogen peroxide, and copper sulfate, as swimming capacity impairment was observed for both strains in the lowest concentrations used in lethal tests. Overall, results showed that IBA3 was more tolerant to the majority of stressors, comparing to MRS10, which may be due to differences in physiological characteristics, highlighting the importance of performing multiclonal experiments. Also, swimming capacity inhibition proved to be a good alternative to the classical lethality tests, being sensitive to lower concentrations and with shorter exposure periods.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Swimming behaviour as an alternative endpoint to assess differences in abiotic stress sensitivities between strains of Brachionus koreanus (Rotifera: Monogononta)

Granada

Lemos

Bossier

et al. 2023

Environ Sci Pollut Res

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“…The O 2 levels were measured using an Extracellular Oxygen Consumption Assay Kit (ab197243) (Abcam) following manufacturer’s protocol. The fluorescent dye used in this assay kit is quenched by oxygen and is excited at 360–380 nm (max 380) and emits at 630–680 nm (max 650), where the higher the fluorescence signal, the less oxygen is in the sample 78,79 . Cells were seeded in a 96-well plate under the same conditions described above, incubated with an O 2 reagent, covered with mineral oil and read in a subsequent time course using a fluorometric microplate reader.…”

Section: Methodsmentioning

confidence: 99%

High Glucose Environments Interfere with Bone Marrow-Derived Macrophage Inflammatory Mediator Release, the TLR4 Pathway and Glucose Metabolism

Ayala

Tessaro

Jannuzzi

et al. 2019

Sci Rep

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Macrophages may be a crucial aspect of diabetic complications associated with the inflammatory response. In this study, we examined how hyperglycaemia, a common aspect of diabetes, modulates bone marrow-derived macrophages (BMDMs) under an inflammatory stimulus. To perform this study, BMDMs from non-diabetic and diabetic (60 mg/kg alloxan, i.v.) male C57BL/6 mice (CEUA/FCF/USP-488) were cultured under normal (5.5 mM) and high glucose (HG, 25 or 40 mM) conditions and stimulated or not stimulated with lipopolysaccharide (LPS, 100 ng/mL). Compared to the BMDMs from the normoglycaemic mice, the LPS-stimulated BMDMs from the diabetic mice presented reduced TLR4 expression on the cell surface, lower phagocytic capacity, and reduced secretion of NO and lactate but greater oxygen consumption and greater phosphorylation of p46 SAPK/JNK, p42 ERK MAPK, pAKT and pPKC-δ. When the BMDMs from the non-diabetic mice were cultured under high-glucose conditions and stimulated with LPS, TLR4 expression was reduced on the cell surface and NO and H 2 O 2 levels were reduced. In contrast, the diabetic BMDMs cultured under high glucose conditions presented increased levels of lactate and reduced phosphorylation of AKT, PKC-δ and p46 SAPK/JNK but enhanced phosphorylation of the p46 subunit of SAPK/JNK after LPS stimulation. High glucose levels appear to modify macrophage behaviour, affecting different aspects of diabetic and healthy BMDMs under the same LPS stimulus. Thus, hyperglycaemia leaves a glucose legacy, altering the basal steady state of macrophages.

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Targeted cytochrome P450 3045C1 (CYP3045C1) gene mutation via CRISPR-Cas9 ribonucleoproteins in the marine rotifer Brachionus koreanus

Kim

Park

et al. 2018

Hydrobiologia

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Body size-dependent interspecific tolerance to cadmium and their molecular responses in the marine rotifer Brachionus spp.

Cited by 31 publications

References 43 publications

Swimming behaviour as an alternative endpoint to assess differences in abiotic stress sensitivities between strains of Brachionus koreanus (Rotifera: Monogononta)

Swimming behaviour as an alternative endpoint to assess differences in abiotic stress sensitivities between strains of Brachionus koreanus (Rotifera: Monogononta)

High Glucose Environments Interfere with Bone Marrow-Derived Macrophage Inflammatory Mediator Release, the TLR4 Pathway and Glucose Metabolism

Targeted cytochrome P450 3045C1 (CYP3045C1) gene mutation via CRISPR-Cas9 ribonucleoproteins in the marine rotifer Brachionus koreanus

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