Body Surface Area of the Golden Syrian Hamster

Ohwada, Kazuo

doi:10.1538/expanim1978.41.2_221

Cited by 18 publications

(10 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The equivalent low, medium and high AC doses for humans are 5.50, 10.99 and 21.99 mg kg −1 body weight per day, respectively, based on the Meeh-Rubner equation. [54,72] Statistical Analysis: The procedures or preparation of ACs and UPLC-ESI-MS analysis; fecal bacterial content DNA extraction and PCR amplification 16S rDNA sequencing; SCFA quantification, pH measurement, and ammonia determination in colonic contents and feces; colon TJ expression by quantitative real-time PCR; hematoxylin and eosin (H&E) staining; immunofluorescence assessment; western blotting; transmission electron microscopy; and gut permeability and inflammation determination are thoroughly described in the Supporting Information. Oneway ANOVA using SPSS 22.0 was used to evaluate statistical significance, and all data are presented as the mean ± SEM.…”

Section: Methodsmentioning

confidence: 99%

Lycium ruthenicum Anthocyanins Attenuate High‐Fat Diet‐Induced Colonic Barrier Dysfunction and Inflammation in Mice by Modulating the Gut Microbiota

Tian

Zhao

Zhang

et al. 2021

Molecular Nutrition Food Res

114

View full text Add to dashboard Cite

Scope: Gut barrier dysfunction and inflammation originating from a dysbiotic gut microbiota (GM) are strongly associated with a high-fat diet (HFD). Anthocyanins from Lycium ruthenicum (ACs) show antiobesity effects through modulating the GM. However, the mechanism linking the antiobesity effects of ACs and GM modulation remains obscure. Methods and results: To investigate the ameliorative effects of ACs on colonic barrier dysfunction and inflammation, mice are fed an HFD with or without ACs at doses of 50, 100, and 200 mg kg −1 for 12 weeks. AC supplementation reduced weight gain, enriched short-chain fatty acid (SCFA)-producing bacteria (e.g., Ruminococcaceae, Muribaculaceae, Akkermansia, Ruminococcaceae_UCG-014, and Bacteroides) and SCFA content, depleted endotoxin-producing bacteria (e.g., Helicobacter and Desulfovibrionaceae), and decreased endotoxin (i.e., lipopolysaccharide) levels. SCFAs substantially activated G protein-coupled receptors (GPRs), inhibited histone deacetylases (HDAC), increased intestinal tight junction mRNA and protein expression levels, reduced intestinal permeability, and protected intestinal barrier integrity in HFD-induced mice. These effects mitigate intestinal inflammation by inhibiting the LPS/NF-B/TLR4 pathway. Conclusion: These data indicates that ACs can mitigate colonic barrier dysfunction and inflammation, induce SCFA production and inhibit endotoxin production by modulating the GM in HFD-fed mice. This finding provides a clue for understanding the antiobesity effects of ACs.

show abstract

Section: Methodsmentioning

confidence: 99%

Lycium ruthenicum Anthocyanins Attenuate High‐Fat Diet‐Induced Colonic Barrier Dysfunction and Inflammation in Mice by Modulating the Gut Microbiota

Tian

Zhao

Zhang

et al. 2021

Molecular Nutrition Food Res

114

View full text Add to dashboard Cite

show abstract

“…TGR5, which is expressed ubiquitously, is one of the widely investigated receptors of BAs 23 . Previous studies of TGR5 focus more on the enteroendocrine L cells in the ileum, which could stimulate the secretion of GLP-1 after activated by BAs, thereby improving liver and pancreatic function, and increasing insulin sensitivity and glucose tolerance 13,14,17 . In the present study, we found FBG was decreased after biliary diversion, glucose tolerance was enhanced and HOMA-IR was decreased.…”

Section: Discussionmentioning

confidence: 99%

“…REE was calculated using the equation: REE (kJ/m 2 h) = Q / S (Q is the quantity of heat per hour, S is the surface area), Q (kJ/ h) = VO 2 (L/h) Á 20.22 (VO 2 is the oxygen consumption per hour, and the corresponding oxygen heat value was 20.22 kJ/ L). Body surface area was calculated using the Meeh-Rubner equation 13 : S (m 2 ) = 0.091 Á W (kg) 2/3 (W is weight).…”

Section: Resting Energy Expenditurementioning

confidence: 99%

Biliary diversion increases resting energy expenditure leading to decreased blood glucose level in mice with type 2 diabetes

Yin

Chen

Dong

et al. 2021

J of Diabetes Invest

View full text Add to dashboard Cite

Aims/Introduction Type 2 diabetes mellitus is a group of metabolism abnormalities in carbohydrates and energy. Our aim was to investigate resting energy expenditure (REE) and blood glucose changes after biliary diversion in mice with diabetes. Materials and Methods Male mice with diabetes were randomly divided into biliary diversion and sham groups. REE was detected by indirect calorimetry, the levels of fasting blood glucose, total bile acids and triiodothyronine were analyzed. After mice were killed, the weight amount of brown adipose tissue (BAT) and gastrocnemius was measured, and the expression level of G protein‐coupled bile acid receptor and type 2 iodothyronine deiodinase in BAT and gastrocnemius were examined. Results The two groups of mice were pair‐fed, the bodyweights (P < 0.001) and the fasting blood glucose level (P < 0.001) in the biliary diversion group significantly decreased 24 weeks after surgery. The intraperitoneal glucose tolerance test (P = 0.035) and oral glucose tolerance test (P = 0.027) showed improvement in glucose tolerance after surgery. The REE level significantly increased 24 weeks after surgery (P = 0.005), the levels of total bile acids (P = 0.014) and triiodothyronine (P < 0.001) increased at the 24th postoperative week. The weight ratio of BAT (P = 0.038) and gastrocnemius (P = 0.026) in the biliary diversion group were higher than that in the sham group. The expression of G protein‐coupled bile acid receptor in BAT (P < 0.001) and gastrocnemius (P = 0.003) were upregulated after surgery, and the type 2 iodothyronine deiodinase expression also increased in BAT (P = 0.015) and gastrocnemius (P = 0.015). Conclusions The REE level increased and the glucose metabolism improved in mice with diabetes after biliary diversion.

show abstract

“…[ 1 ] In this work, experimental groups (3 week OVT, 8 week OVT) were administered OVT at a dose of 50 mg kg −1 d −1 , which was calculated based on the Meeh–Rubner equation. [ 13 ] The control groups (3 week control, 8 week control) were administered an equivalent volume of distilled water. Since the dose of OVT supplement was much lower than the dietary protein content from feed (5 g kg −1 d −1 ), the influence of OVT supplement on the total protein content in chow is negligible.…”

Section: Methodsmentioning

confidence: 99%

Multi‐Omics Analysis of the Effects of Egg Ovotransferrin on the Gut Environment in Mice: Mucosal Gene Expression, Microbiota Composition, and Intestinal Structural Homeostasis

Zhang

Tan

Yang

et al. 2020

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Scope: Egg ovotransferrin (OVT) is considered a functional food ingredient for its various bioactivities. The objective of this work is to explore the potential biological activity of OVT on the gut health. Methods and results: Both young (3 week old) and adult (8 week old) mouse models are utilized in this research. Each group receives a standard diet containing either OVT (experimental group) or distilled water (control group) for a 14 day period. Transcriptome and 16S rDNA sequencing analyses are applied to characterize the gene expression in colonic epithelial cells and gut microbiota composition. In the young groups, OVT suppresses the genes correlated with lipid metabolism and signal transduction. The regulated genes in the adult groups encompass various biological processes, including lipid metabolism, signal transduction, endocrine system, and others. OVT increases the proportion of some beneficial bacteria significantly, especially Akkermansia, and inhibits some harmful bacteria. Furthermore, OVT affects mucosal morphology positively via increasing the crypt depth. OVT also increases the expression of tight junction protein occludin by 3.0-and 5.2-folds in young and adult groups, respectively. Conclusion: OVT exhibits some beneficial effects on the gut environment. These positive findings provide new insight into the understanding of OVT as an excellent functional ingredient.

show abstract

Body Surface Area of the Golden Syrian Hamster

Cited by 18 publications

References 2 publications

Lycium ruthenicum Anthocyanins Attenuate High‐Fat Diet‐Induced Colonic Barrier Dysfunction and Inflammation in Mice by Modulating the Gut Microbiota

Lycium ruthenicum Anthocyanins Attenuate High‐Fat Diet‐Induced Colonic Barrier Dysfunction and Inflammation in Mice by Modulating the Gut Microbiota

Biliary diversion increases resting energy expenditure leading to decreased blood glucose level in mice with type 2 diabetes

Multi‐Omics Analysis of the Effects of Egg Ovotransferrin on the Gut Environment in Mice: Mucosal Gene Expression, Microbiota Composition, and Intestinal Structural Homeostasis

Contact Info

Product

Resources

About