Bone calcium/phosphorus ratio determination using dual energy X-ray method

Sotiropoulou, P.; Fountos, G.; Martini, N.; Koukou, V.; Michail, C.; Kandarakis, I.; Nikiforidis, Georgios

doi:10.1016/j.ejmp.2015.01.019

Cited by 41 publications

(30 citation statements)

References 24 publications

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“…Blood calcium plays an important role in the process of blood coagulation (Wildgoose et al, ). The bone calcium content reflects the amount of bone calcium deposition that has a significant effect on the growth performance in piglets (Masuyama et al, ; Sotiropoulou et al, ). To our knowledge, this is the first study to demonstrate that blood calcium tends to go up initially and then descend with increasing of Ca/AP ratios, reaching the maximum at the calcium to available phosphorus ratio of 2:1, and this provides evidence that this ratio directly affects the normal development of piglet bone.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary calcium to available phosphorus ratios on bone metabolism and osteoclast activity of the OPG /RANK/RANKL signalling pathway in piglets

Zhao

Sun

2019

Animal Physiology Nutrition

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Hydroxyapatite, a mineral form of calcium (Ca) and phosphorus (P) that gives bones their rigidity, is the major and essential component of bones and teeth in the human and animal body. A suitable ratio of Ca and P is vital for bone growth. The aim of this study was to explore the effects of dietary calcium to available phosphorus ratios (Ca/AP) on bone metabolism and osteoclast activity of the osteoprotegerin (OPG)/receptor activator of nuclear factor kappa B ligand (RANKL) signalling pathway in piglets. At days 15 and 29, the piglets were assessed for growth performance, blood indicators, cytokines and the OPG/RANK/RANKL signalling pathway. Our results showed that piglets fed a dietary Ca/AP ratio of 2:1 increases growth performance and regulates blood indicators and cytokines (parathyroid hormone (PTH), calcitonin (CT), vitamin D3 (VD3), insulin‐like growth factor‐1 (IGF‐1), transforming growth factor‐β (TGF‐β), interleukin‐1 (IL‐1), interleukin‐6 (IL‐6), carboxyterminal propeptide of type I procollagen (PICP), tartrate‐resistant acid phosphatase (TRACP), alkaline phosphatase (ALP) and osteocalcin (OCN) content). We also demonstrated that this ratio affects hormone secretion and further bone metabolism through the OPG/RANK/RANKL signalling pathway of osteoclasts. These results indicate that a suitable dietary Ca/AP ratio is vital for bone growth and reduce the incidence of bone diseases such as osteoporosis, providing a practical basis for the raising of piglets.

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Section: Discussionmentioning

confidence: 99%

Effects of dietary calcium to available phosphorus ratios on bone metabolism and osteoclast activity of the OPG /RANK/RANKL signalling pathway in piglets

Zhao

Sun

2019

Animal Physiology Nutrition

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“…Usually, the titanium alloys and the different coatings used to enhance their osseointegration, were studied for a period of 2, 4, 6 and 12 weeks after implantation implantation, but none studied the behavior of the alloys for more that 18 weeks [ 11 ], thus we considerered that a longer period of time (six months), would be more appropriate for integration assessment. It is a known fact that mineralization and demineralization balance require time to be achieved [ 12 ]. This can explain why the demineralization process was continuous, for the Ti 6 Al 7 Nb sample with a peak at about 3 months, followed by remineralization of the site within the following months, reaching a percentage of mineralized bone for 6 months comparable with the one at 1 month (94.67% vs. 93.83%).…”

Section: Discussionmentioning

confidence: 99%

Micro-CT and histological analysis of Ti6Al7Nb custom made implants with hydroxyapatite and SiO2-TiO2 coatings in a rabbit model

Armencea

Berce²,

Rotaru

et al. 2015

Medicine and Pharmacy Reports

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Background and aimBone defect reconstruction in the maxillofacial area comes as a necessity after traumatic, oncological or congenital pathology. Custom made implant manufacturing, such as selective laser melting (SLM), is very helpful when bone reconstruction is needed. In the present study we assessed the osseointegration of custom made implants made of Ti6Al7Nb with two different coatings: SiO2-TiO2 and hydroxyapatite, by comparing the bone mineral density (BMD) measured on micro-CT and the histological mineralized bone surrounding the implants.MethodsCustom made – cylindrical type – implants were produced by selective laser melting, coated with SiO2-TiO2 and hydroxyapatite and implanted in the rabbit femur. The animals (divided into 3 groups) were sacrificed at 1, 3 and 6 months and the implants were removed together with the surrounding bone. Bone mineral density and histological examination of the bone-implant surface was performed for each group.ResultsBMD and histological examination of the samples determined the quantity of mineralized bone at the implant site, showing a good percentage of mineralized bone for the coated implants at 1, 3 and 6 months. The measurements for the implants without coating showed a significant lower quantity of mineralized bone at 3 months compared with the implants with coating, and a good quantity of mineralized bone at 6 months, showing a process of demineralization followed by remineralization in the last month. The measurements of BMD showed similar results with the histological examination.ConclusionsThe use of micro-CT and the measurement of BMD are a reliable, minimally invasive and a quick method of osseointegration assessment.

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“…The Ca/P ratios were found to be close to those of reference bone (Ca/P = 1.37, Table 3), although they cannot be solely used as a criterion [96,97]. FTIR spectra of trial BP-1 showed an increase in both phosphate and carbonate type A and B peaks, suggesting that c-HAP is formed; on the other hand, in trials (BP-2, 3, and 4), the product quantities are negligible, as compared with the initial bone spectrum (Fig.…”

Section: Pressed Bone Powder Disks (Trials Bp)mentioning

confidence: 69%

Optimizing the biomimetic synthesis of hydroxyapatite for the consolidation of bone using diammonium phosphate, simulated body fluid, and gelatin

et al. 2020

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Strategies involving the synthesis of hydroxyapatite (HAP) are significant in health and materials sciences, and lately, in heritage science. Conditions for the optimized heterogeneous formation of HAP for consolidating bone are investigated using a simple methodology with discs of calcium-containing starting materials and phosphate-containing reagent solutions; the results were evaluated against whole bone specimens. Diammonium phosphate (DAP) solutions with simulated body fluid (SBF) and gelatin as cofactors in all combinations were used in trials, while the products were characterized by microscopy, elemental microanalysis, X-Ray diffractometry, and Fourier transform infrared spectroscopy. The hypothesis that bone, SBF, and gelatin may biomimetically promote HAP formation was tested through a simple experimental design. The results showed that HAP is generally formed in mixtures with octacalcium phosphate (OCP), as well as calcium hydrogen phosphate dihydrate (brushite). The precipitation of carbonate HAP signals the formation of a biocompatible product in the cases where SBF and gelatin were employed as cofactors. Gelatin was found to further promote the product formation in most cases. It was shown that DAP, as well as its combination with gelatin, could predominantly form HAP in most trials, while OCP is co-crystallized in SBF-containing solutions.

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Bone calcium/phosphorus ratio determination using dual energy X-ray method

Cited by 41 publications

References 24 publications

Effects of dietary calcium to available phosphorus ratios on bone metabolism and osteoclast activity of the OPG /RANK/RANKL signalling pathway in piglets

Effects of dietary calcium to available phosphorus ratios on bone metabolism and osteoclast activity of the OPG /RANK/RANKL signalling pathway in piglets

Micro-CT and histological analysis of Ti6Al7Nb custom made implants with hydroxyapatite and SiO2-TiO2 coatings in a rabbit model

Optimizing the biomimetic synthesis of hydroxyapatite for the consolidation of bone using diammonium phosphate, simulated body fluid, and gelatin

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