The behavior, growth and development, and production performance of poultry are affected by the light environment. The influence of light results from a combination of light sources, light intensity, light color, and the photoperiod regimen. With light-emitting diode (
LED
) lamps applied in poultry housing systems, specific light colors are desired for each time period for layer chickens. The objective of this study was to investigate the effects of a 2-phase mixed color lighting program (phase 1: blue-green, 1 D–13 wk; phase 2: yellow-orange, 14–20 wk) using LED lights on the blood parameters, skeletal development parameters, and sexual development parameters of caged layer chickens during their brooding and rearing periods. Fifty-two chickens were raised from 1 D to 20 wk of age in each of the 4 treatment groups with 3 replicates, with white (400–700 nm) light at phase 1 and phase 2 (
WL
treatment as the control); blue–green (435–565 nm) light at phase 1 followed by yellow–orange (565–630 nm) light at phase 2 (
BG-YOL
treatment); yellow–orange LED (565–630 nm) light at phase 1 and phase 2 (
YOL
treatment); and blue–green (435–565 nm) light at phase 1 and phase 2 (
BGL
treatment). The results showed that the serum Ig concentrations of the layer chickens in the BG-YOL treatment and BGL treatment were higher than those in the WL treatment at 13 wk of age (
P
< 0.05). At the age of 20 wk, the serum glucose concentration levels of the pullets after the WL and BGL treatments were lower than those after the YOL treatment (
P
< 0.05). Compared with the WL treatment, the YOL treatment significantly increased the bone mineral density of the layer chickens (
P
< 0.05), and BG-YOL treatment promoted the development of the sexual organs (oviducts and ovaries) of the laying hens at the age of 20 wk (
P
< 0.05). For the 50% egg production age, the YOL treatment was earlier than the other 3 treatments. This study demonstrated that appropriately staged spectral control using LED lights could have positive effects on the immune performance, bone development, and production performance of caged layer chickens during their brooding and rearing periods.