Bone engineering with adipose tisssue derived stromal cells

Weinzierl, Konstanze; Hemprich, Alexander; Frerich, Bernhard

doi:10.1016/j.jcms.2006.07.860

Cited by 51 publications

(36 citation statements)

References 18 publications

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“…The stem cell character of these cells had been proven earlier by successful osteogenic, adipogenic and smooth muscle differentiation. [19,34] The cells were split in a 1:4 ratio and amplified up to the 3 rd passage. Sterilization of the samples was accomplished with g-irradiation with the aid of a 60…”

Section: Cultivation Of Adipose Tissue-derived Stem Cells and Sterilimentioning

confidence: 99%

Ring‐Opening Metathesis Polymerization‐Based Synthesis of CaCO₃ Nanoparticle‐Reinforced Polymeric Monoliths for Tissue Engineering

Weichelt

Frerich

Lenz

et al. 2010

Macromol. Rapid Commun.

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Porous monolithic materials have been prepared via ring-opening metathesis polymerization from norborn-2-ene and a 7-oxanorborn-2-ene-based cross-linker in the presence of porogenic solvents (i.e., 2-propanol and toluene) and norborn-2-enephosphonate surface-modified CaCO(3) nanoparticles, using the 3(rd) -generation Grubbs-initiator RuCl(2) (Py)(2) (IMesH(2) )(CHPh). The experimental setup and the conditions chosen allowed for the manufacturing of polymeric monoliths characterized by a homogeneous distribution of the inorganic nanoparticles throughout the polymeric monolith. Depending on the nanoparticle content, the macropore diameters could be varied in the 30-120 µm regime. Noteworthy, the addition of nanoparticles did not affect the phase separation-triggered formation of the monolithic matrix nor the meso- and microporosity as evidenced by N(2) -adsorption experiments.

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Section: Cultivation Of Adipose Tissue-derived Stem Cells and Sterilimentioning

confidence: 99%

Ring‐Opening Metathesis Polymerization‐Based Synthesis of CaCO₃ Nanoparticle‐Reinforced Polymeric Monoliths for Tissue Engineering

Weichelt

Frerich

Lenz

et al. 2010

Macromol. Rapid Commun.

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“…Adipose tissue represents an abundant and accessible source of adult stem cells that can be differentiated into osteogenic [Im et al, 2005;Shi et al, 2005;Weinzierl et al, 2006;Qu et al, 2007], adipogenic [Gimble and Guilak, 2003;Qu et al, 2007], chondrogenic [Huang et al, 2004;Qu et al, 2007], and myogenic cells in a lineage-specific culture medium [Zuk et al, 2001;Rada et al, 2009]. Adipose tissue-derived MSCs (AdMSCs) possess a number of advantages over bone marrow MSCs (BMSCs), including a sufficient supply with minimally invasive procurement [Rada et al, 2009].…”

Section: Introductionmentioning

confidence: 99%

Sustained Release of 17β-Estradiol Stimulates Osteogenic Differentiation of Adipose Tissue-Derived Mesenchymal Stem Cells on Chitosan-Hydroxyapatite Scaffolds

Irmak

Demirtaş

Altındal

et al. 2014

Cells Tissues Organs

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The aim of this study was to develop a 17β-estradiol (E2)-releasing scaffold-nanoparticle system in order to promote osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells (AdMSCs) for bone tissue regeneration. E2-loaded poly(lactide-co-glycolide) (PLGA) nanoparticles with a diameter of ∼240 nm were produced via an emulsion-diffusion-evaporation method. Because of its higher encapsulation efficiency (54%), PLGA, which has a 65:35 composition, was chosen for the preparation of nanoparticles. Chitosan-hydroxyapatite (HA) scaffolds in macroporous structures with interconnected pores were prepared by combining microwave irradiation and gas-foaming techniques. PLGA nanoparticles were loaded onto scaffolds in 2 ways: via embedding after scaffold fabrication and during fabrication. While 100% of the loaded E2 was released during 55 days from scaffolds loaded by embedding, a controlled release behavior of E2 was observed over 135 days in scaffolds loaded during manufacture. The results of cell culture studies indicated that the controlled delivery of E2 from PLGA nanoparticles loaded on chitosan-HA scaffolds had a significant effect on the osteogenic differentiation of AdMSCs.

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“…The results presented in this case report may probably be justified because the fat tissue is a source of stem cells 11) , containing progenitor mesenchymal cells that may be used in tissue engineering for bone regeneration 9) . These cells have the ability to differentiate into osteoblasts, indicating their potential in human bone tissue engineering.…”

Section: Discussionmentioning

confidence: 87%

Autogenous Bone Graft Combined with Buccal Fat Pad as Barrier in Treatment of Class II Furcation Defect: A Case Report

Deliberador

Mendes

Storrer

et al. 2012

Bull. Tokyo Dent. Coll.

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The treatment of furcation defects is a complex and difficult task that may compromise the success of periodontal therapy. Here we report a new clinical treatment of a Class II furcation defect using an autogenous bone graft associated with a buccal fat pad (BFP) used as a membrane. The surgical treatment was performed following initial periodontal therapy. Post-operative follow-up appointments were performed at 3, 7, and 12 months. Clinically, after 3 and 7 months, a reduction in probing depth without bleeding on probing and an increase in vertical and horizontal clinical attachment level were observed. After 7 post-operative months, an increase in keratinized gingiva was observed. Radiographically, a significant improvement was noted, with the furcation defect almost completely closed. These results could also be observed after 12 postoperative months. It can be concluded that the combined use of autogenous bone graft and a BFP yielded clinically favorable outcome in the treatment of a mandibular Class II furcation defect.

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Bone engineering with adipose tisssue derived stromal cells

Cited by 51 publications

References 18 publications

Ring‐Opening Metathesis Polymerization‐Based Synthesis of CaCO₃ Nanoparticle‐Reinforced Polymeric Monoliths for Tissue Engineering

Ring‐Opening Metathesis Polymerization‐Based Synthesis of CaCO₃ Nanoparticle‐Reinforced Polymeric Monoliths for Tissue Engineering

Sustained Release of 17β-Estradiol Stimulates Osteogenic Differentiation of Adipose Tissue-Derived Mesenchymal Stem Cells on Chitosan-Hydroxyapatite Scaffolds

Autogenous Bone Graft Combined with Buccal Fat Pad as Barrier in Treatment of Class II Furcation Defect: A Case Report

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Bone engineering with adipose tisssue derived stromal cells

Cited by 51 publications

References 18 publications

Ring‐Opening Metathesis Polymerization‐Based Synthesis of CaCO3 Nanoparticle‐Reinforced Polymeric Monoliths for Tissue Engineering

Ring‐Opening Metathesis Polymerization‐Based Synthesis of CaCO3 Nanoparticle‐Reinforced Polymeric Monoliths for Tissue Engineering

Sustained Release of 17β-Estradiol Stimulates Osteogenic Differentiation of Adipose Tissue-Derived Mesenchymal Stem Cells on Chitosan-Hydroxyapatite Scaffolds

Autogenous Bone Graft Combined with Buccal Fat Pad as Barrier in Treatment of Class II Furcation Defect: A Case Report

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Ring‐Opening Metathesis Polymerization‐Based Synthesis of CaCO₃ Nanoparticle‐Reinforced Polymeric Monoliths for Tissue Engineering

Ring‐Opening Metathesis Polymerization‐Based Synthesis of CaCO₃ Nanoparticle‐Reinforced Polymeric Monoliths for Tissue Engineering