Bone Marrow and Adipose-Derived Mesenchymal Stem Cells Alleviate Methotrexate-Induced Pulmonary Fibrosis in Rat: Comparison with Dexamethasone

Fikry, Ebtehal Mohammad; Safar, Marwa M.; Hasan, Wedad A.; Fawzy, Hala M.; El-Denshary, Ezz-El-Din S.

doi:10.1002/jbt.21701

Cited by 26 publications

(21 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The authors showed that AD-MSCs had more potential in terms of restoration of hyaluronic acid and suppression of excessive collagen deposition (Hiwatashi et al, 2014;Hiwatashi et al, 2016). Both BM-MSCs and AD-MSCs were considered to have similar potential for treating methotrexate-induced pulmonary fibrosis in rat (Fikry et al, 2015). In a rabbit model of tendon repair, AD-MSCs also exhibited greater treatment potential than BM-MSCs (Behfar et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Adipose derived stem cell transplantation is better than bone marrow mesenchymal stem cell transplantation in treating hindlimb ischemia in mice

Vu¹,

Phi²,

Dao³

et al. 2016

Biomed Res Ther

View full text Add to dashboard Cite

Abstract-Introduction:Bone marrow derived MSCs (BM-MSCs) and adipose derived MSCs (AD-MSCs) are among the types of stem cells most commonly studied. Our study aims to compare the therapeutic efficacy of allograft AD-MSCs versus BM-MSCs in a mouse model of hindlimb ischemia. Methods: AD-MSCs were isolated from belly fat and BM-MSCs were isolated from femur bone marrow. They were used to treat mice with acute hindlimb ischemia. Treatment efficacy was compared among 4 groups: injected with BM-MSCs, injected with ADMSCs, non-treated and injected with phosphate buffered saline. Mice in the groups were evaluated for the following: necrosis grade of leg, leg edema, blood flow, muscle cell restructure and new blood vessel formation. Results: Results showed that AD-MSC transplantation significantly recovered acute limb ischemia, with 76.5% of mice fully recovered, while the ratio was only 48.5% in BM-MSC transplanted group, and 0% in the non-treated and PBS groups. Evaluation of leg edema, blood flow, muscle cell restructure and new blood vessel formation also supported the observation that AD-MSC transplantation was superior over BM-MSC transplantation. Conclusion: Therefore, AD-MSCs may serve as the more suitable MSC for hindlimb ischemia treatment and angiogenesis therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Adipose derived stem cell transplantation is better than bone marrow mesenchymal stem cell transplantation in treating hindlimb ischemia in mice

Vu¹,

Phi²,

Dao³

et al. 2016

Biomed Res Ther

View full text Add to dashboard Cite

show abstract

“…29 In brief, the skin tissues were removed of blood, rapidly frozen in liquid nitrogen and stored at −80°C. 29 In brief, the skin tissues were removed of blood, rapidly frozen in liquid nitrogen and stored at −80°C.…”

Section: Hydroxyproline Contentmentioning

confidence: 99%

“…The hydroxyproline content in the irradiated skin tissues was used to quantify the collagen content using the hydroxyproline analysis kit (Nanjing Jiancheng Bioengineering Institute, A030-2) according to the manufacturer's protocol, since collagen contains approximately 13.5% hydroxyproline. 29 In brief, the skin tissues were removed of blood, rapidly frozen in liquid nitrogen and stored at −80°C. The results are reported as micrograms of hydroxyproline per gram of tissue wet weight.…”

Section: Hydroxyproline Contentmentioning

confidence: 99%

Establishment and characterization of a radiation‐induced dermatitis rat model

Sheng

Zhou

Wang

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

Radiation‐induced dermatitis is a common and serious side effect after radiotherapy. Current clinical treatments cannot efficiently or fully prevent the occurrence of post‐irradiation dermatitis, which remains a significant clinical problem. Resolving this challenge requires gaining a better understanding of the precise pathophysiology, which in turn requires establishment of a suitable animal model that mimics the clinical condition, and can also be used to investigate the mechanism and explore effective treatment options. In this study, a single dose of 90 Gy irradiation to rats resulted in ulceration, dermal thickening, inflammation, hair follicle loss, and sebaceous glands loss, indicating successful establishment of the model. Few hair follicle cells migrated to form epidermal cells, and both the severity of skin fibrosis and hydroxyproline levels increased with time post‐irradiation. Radiation damaged the mitochondria and induced both apoptosis and autophagy of the skin cells. Therefore, irradiation of 90 Gy can be used to successfully establish a rat model of radiation‐induced dermatitis. This model will be helpful for developing new treatments and gaining a better understanding of the pathological mechanism of radiation‐induced dermatitis. Specifically, our results suggest autophagy regulation as a potentially effective therapeutic target.

show abstract

“…Methotrexate has a broad spectrum of side effects, including hematological, gastrointestinal, hepatic and renal toxicities as well as lung fibrosis and cardiac damage . Various preclinical studies investigated regenerative effects of MSCs on methotrexate‐induced cardiac and pulmonary fibrosis, kidney and liver injury and have shown beneficial effects regarding organ regeneration and parenchymal functions . However, as most animal work has been done with allogeneic MSCs, further studies are required to investigate if the observed benefits can also be reproduced with pre‐exposed autologous stem cells.…”

Section: Effects Of Chemotherapeutic Anti‐cancer Agents On Mscsmentioning

confidence: 99%

The current understanding of mesenchymal stem cells as potential attenuators of chemotherapy‐induced toxicity

Rühle

Huber

Saffrich

et al. 2018

Intl Journal of Cancer

View full text Add to dashboard Cite

Chemotherapeutic agents are part of the standard treatment algorithms for many malignancies; however, their application and dosage are limited by their toxic effects to normal tissues. Chemotherapy-induced toxicities can be long-lasting and may be incompletely reversible; therefore, causative therapies for chemotherapy-dependent side effects are needed, especially considering the increasing survival rates of treated cancer patients. Mesenchymal stem cells (MSCs) have been shown to exhibit regenerative abilities for various forms of tissue damage. Preclinical data suggest that MSCs may also help to alleviate tissue lesions caused by chemotherapeutic agents, mainly by establishing a protective microenvironment for functional cells. Due to the systemic administration of most anticancer agents, the effects of these drugs on the MSCs themselves are of crucial importance to use stem cell-based approaches for the treatment of chemotherapy-induced tissue toxicities. Here, we present a concise review of the published data regarding the influence of various classes of chemotherapeutic agents on the survival, stem cell characteristics and physiological functions of MSCs. Molecular mechanisms underlying the effects are outlined, and resulting challenges of MSC-based treatments for chemotherapy-induced tissue injuries are discussed.

show abstract

Bone Marrow and Adipose-Derived Mesenchymal Stem Cells Alleviate Methotrexate-Induced Pulmonary Fibrosis in Rat: Comparison with Dexamethasone

Cited by 26 publications

References 42 publications

Adipose derived stem cell transplantation is better than bone marrow mesenchymal stem cell transplantation in treating hindlimb ischemia in mice

Adipose derived stem cell transplantation is better than bone marrow mesenchymal stem cell transplantation in treating hindlimb ischemia in mice

Establishment and characterization of a radiation‐induced dermatitis rat model

The current understanding of mesenchymal stem cells as potential attenuators of chemotherapy‐induced toxicity

Contact Info

Product

Resources

About