Bone Marrow and Peripheral Blood Stem Cell Transplantation

Benn, Howard; Rowley, Scott D.

doi:10.1016/b978-0-443-06981-9.50063-6

Cited by 1 publication

(1 citation statement)

References 379 publications

(201 reference statements)

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“…These patients would benefit from bridging therapy until the transplanted immune system can mount a sufficient response to infection or reactivation. Transplant engraftment takes up to a month, and, while infections occur during cytopenia and after, this does not allow sufficient time to develop an adaptive immune response [ 18 ]. Therefore, physicians observe a vulnerable phase of considerable length.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Bispecific T-Cell Engagers Targeting Murine Cytomegalovirus

Menschikowski,

Bednar,

Kübel

et al. 2024

Viruses

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Human cytomegalovirus is a ubiquitous herpesvirus that, while latent in most individuals, poses a great risk to immunocompromised patients. In contrast to directly acting traditional antiviral drugs, such as ganciclovir, we aim to emulate a physiological infection control using T cells. For this, we constructed several bispecific T-cell engager (BiTE) constructs targeting different viral glycoproteins of the murine cytomegalovirus and evaluated them in vitro for their efficacy. To isolate the target specific effect without viral immune evasion, we established stable reporter cell lines expressing the viral target glycoprotein B, and the glycoprotein complexes gN-gM and gH-gL, as well as nano-luciferase (nLuc). First, we evaluated binding capacities using flow cytometry and established killing assays, measuring nLuc-release upon cell lysis. All BiTE constructs proved to be functional mediators for T-cell recruitment and will allow a proof of concept for this treatment option. This might pave the way for strikingly safer immunosuppression in vulnerable patient groups.

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Section: Introductionmentioning

confidence: 99%