Bone marrow mesenchymal stem cell-derived exosomal microRNA-125a promotes M2 macrophage polarization in spinal cord injury by downregulating IRF5

Chang, Qing; Hao, Yupeng; Wang, Yifan; Zhou, Yingjie; Zhuo, Hanjie; Zhao, Gang

doi:10.1016/j.brainresbull.2021.02.015

Cited by 63 publications

(47 citation statements)

References 30 publications

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“…A total of 701 related articles were obtained. After excluding the literature based on the exclusion criteria, eventually 32 studies were included ( 19 – 21 , 28 – 56 ). The entire screening process is summarized in Figure 1 .…”

Section: Resultsmentioning

confidence: 99%

“…Among the included 32 studies, only two studies were controlled studies ( 29 , 33 ), and the remaining 30 studies were randomized controlled studies. The species of animals included SD rats ( 19 – 21 , 28 – 37 , 39 , 40 , 42 – 44 , 46 , 47 , 49 – 53 , 55 , 56 ), Wistar rats ( 41 , 54 ), and C57BL/6 mice ( 38 , 45 , 48 ); gender included male ( 28 , 30 – 32 , 38 – 45 , 49 , 53 , 54 ) and female ( 19 , 20 , 29 , 33 , 34 , 36 , 37 , 46 – 48 , 50 – 52 , 55 , 56 ), two studies did not report the sex of the animals ( 21 , 35 ); the weights of animals were between 150 ( 41 ) and 300 g ( 20 , 44 ), the weight of the mouse was between 17 and 22 g ( 48 ), and seven of the studies did not report the weight of the animals ( 21 , 29 , 30 , 38 , 42 , 45 , 50 ); age was between 6 ( 38 , 44 , 45 , 50 , 51 ) and 12 weeks ( 47 , 56 ), and nine of the studies did not report the age of the animals ( …”

Section: Resultsmentioning

confidence: 99%

“…Six studies reported types of exosomes but did not report specific sources ( 31 , 32 , 38 , 40 , 45 , 46 ). The transplantation route of exosomes included tail vein ( 19 – 21 , 29 – 32 , 34 – 50 , 52 – 56 ), intranasally ( 33 ) and subcutaneous ( 51 ), and one study did not report the route of exosome transplantation ( 28 ); the time transplantation of exosome ranged from 0 ( 19 , 20 , 31 , 38 , 41 , 44 – 47 , 49 ) to 24 h ( 42 , 43 ) after modeling, one study did not report the time of exosome transplantation ( 40 ); dose of exosomes ranged from 20 ( 29 ) to 200 μg ( 28 , 30 – 33 , 36 – 38 , 41 , 43 , 45 – 49 , 53 , 56 ); the control group includes PBS ( 19 – 21 , 28 – 32 , 34 , 35 , 38 , 39 , 41 , 43 – 51 , 53 – 56 ), saline ( 33 , 36 , 37 , 40 , 42 ), and blank ( 52 ). Basic information of the study subjects was summarized in Supplementary Table 2 .…”

Section: Resultsmentioning

confidence: 99%

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Therapeutic Effect of Exosomes Derived From Stem Cells in Spinal Cord Injury: A Systematic Review Based on Animal Studies

et al. 2022

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ObjectiveA systematic review of the role of stem cell-derived exosomes in repairing spinal cord injury (SCI) and the existing problems in animal experiments to provide a reference for better animal experiments and clinical studies in the future.MethodThree electronic databases, namely PubMed, Web of Science, and Ovid-Embase were searched. The studies were retrieved from inception to October 2021. Two researchers independently screened the literature, extracted data, and evaluated the methodological quality based on the inclusion criteria.Results and DiscussionThirty-two studies were incorporated into the final analyses. Exosomes derived from stem cells could not only significantly improve the motor function of animals with SCI, but also significantly increase the expression of anti-inflammatory factors IL-4 and IL-10 and anti-apoptotic protein Bcl-2, while significantly lowering the pro-inflammatory factor IL-1β and TNF-α and the expression of the apoptotic protein BAX. However, the mechanism of exosome-mediated SCI repair, as well as the best source and dosage remain unknown. In addition, there are still some issues with the design, implementation, and reporting of animal experiments in the included studies. Therefore, future research should further standardize the implementation and reporting of animal studies and fully explore the best strategies for exosomes to repair SCI so as to promote the translation of preclinical research results to clinical research better and faster.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Therapeutic Effect of Exosomes Derived From Stem Cells in Spinal Cord Injury: A Systematic Review Based on Animal Studies

et al. 2022

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“…Other miRNAs like miR-125a derived from bone marrow mesenchymal stem cells (BMMSC) exerts neuroprotective effects by targeting and negatively regulating Interferon Regulatory Factor 5 ( IRF5 ) expression in SCI rats [ 89 ]. MiR-124–3p derived from BMMSCs attenuated nerve injury induced by regulating endoplasmic reticulum to nucleus signaling 1 ( Ern1 ) and M2 macrophage polarization [ 90 ].…”

Section: Mscs Derived Exosomal Mirnas and Scimentioning

confidence: 99%

Potential of different cells-derived exosomal microRNA cargos for treating spinal cord injury

Pan

Liu

Zhu

et al. 2021

Journal of Orthopaedic Translation

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Spinal cord injury (SCI) is a disastrous situation that affects many patients worldwide. A profound understanding of the pathology and etiology of SCI is of great importance in inspiring new therapeutic concepts and treatment. In recent years, exosomes, which are complex lipid membrane structures secreted nearly by all kinds of plants and animal cells, can transport their valuable cargoes (e.g., proteins, lipids, RNAs) to the targeted cells and exert their communication and regulation functions, which open up a new field of treatment of SCI. Notably, the exosome's advantage is transporting the carried material to the target cells across the blood–brain barrier and exerting regulatory functions. Among the cargoes of exosomes, microRNAs, through the modulation of their mRNA targets, emerges with great potentiality in the pathological process, diagnosis and treatment of SCI. In this review, we discuss the role of miRNAs transported by different cell-derived exosomes in SCI that are poised to enhance SCI-specific therapeutic capabilities of exosomes.

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“…Brain micro vascular endothelium is a thin layer of connecting and anchoring dependent cells, which constitutes the interface between blood and solid vessel wall [2] . This vascular endothelium is a highly active metabolic system, which synthesizes various vascular regulatory factors to regulate the microcirculation of brain tissue [3] . Brain micro vascular endothelial cells are now considered as a highly active metabolic system, which synthesizes various substances to nourish nerves and regulate vascular motor function.…”

mentioning

confidence: 99%

Molecular Mechanism of Bone Marrow Mesenchymal Stem Cell Derived Exosomes on Vascular Repair in Rats with Brain Injury

Ma¹,

Cao²,

Sun³

et al. 2021

ijps

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To explore the molecular mechanism of exosomes derived from bone marrow mesenchymal stem cells on vascular repair in rats with brain injury. 30 Sprague-Dawley rats were used in this study. Bone marrow was extracted from the posterior iliac crest of the rat under general anesthesia and bone marrow mesenchymal stem cells were isolated and cultured. Some rats were treated with middle cerebral artery occlusion surgery to induce acute brain injury models. The protein expressions of vascular endothelial growth factor A and vascular endothelial growth factor receptor 2 were analyzed by western blot. Polymerase chain reaction was used to analyze the messenger RNA expressions of endothelin-1, vascular cell adhesion molecule 1 and endothelial nitric oxide synthase related to endothelial cell damage. The protein expressions of vascular endothelial growth factor A and vascular endothelial growth factor receptor 2 in the model group was lower than that in the control group (p<0.05) and the protein expressions in the treatment group was higher than that in the model group (p<0.05). The messenger RNA expressions of endothelin-1 and vascular cell adhesion molecule 1 in the model group increased while the expression of endothelial nitric oxide synthase messenger RNA decreased (p<0.05). The messenger RNA expressions of endothelin-1 and vascular cell adhesion molecule 1 in the treatment group decreased and the messenger RNA expression of endothelial nitric oxide synthase increased (p<0.05). The apoptosis level of endothelial cells in the model group increased and in the treatment group was reduced. Exosomes derived from bone marrow mesenchymal stem cells could effectively regulate cell survival, promote angiogenesis, protect nerve cells and improve the prognosis of stroke in rats by up regulating the expression of C-X-C chemokine receptor type 4 in bone marrow mesenchymal stem cells.

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Bone marrow mesenchymal stem cell-derived exosomal microRNA-125a promotes M2 macrophage polarization in spinal cord injury by downregulating IRF5

Cited by 63 publications

References 30 publications

Therapeutic Effect of Exosomes Derived From Stem Cells in Spinal Cord Injury: A Systematic Review Based on Animal Studies

Therapeutic Effect of Exosomes Derived From Stem Cells in Spinal Cord Injury: A Systematic Review Based on Animal Studies

Potential of different cells-derived exosomal microRNA cargos for treating spinal cord injury

Molecular Mechanism of Bone Marrow Mesenchymal Stem Cell Derived Exosomes on Vascular Repair in Rats with Brain Injury

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